Optimization of Exopolysaccharide (EPS) Production by Rhodotorula mucilaginosa sp. GUMS16

Okoro, Oseweuba Valentine; Gholipour, Amir Reza; Sedighi, Faezeh; Shavandi, Amin; Hamidi, Masoud

doi:10.3390/chemengineering5030039

Cited by 18 publications

(8 citation statements)

References 53 publications

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“…The basidiomycetous Cryptococcus and Tremella spp., Rhodotorula minuta and Trichosporum asahii (Table 1), all produce glucuronic acid (GlcA), and the ascomycetous C. tropicalis produces uronic acids which were not yet identified (Table 2, Ref. [88,90,113,123,124]). Additionally, the exopolysaccharides from both groups of yeasts also largely differ in MW (Table 1), with values globally ranging from ≤8 kDa to >7 MDa (Fig.…”

Section: Yeasts' Ecm Exopolysaccharidesmentioning

confidence: 99%

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

Lucas¹,

Silva²

2023

Front. Biosci. (Elite Ed)

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Microbes are traditionally regarded as planktonic organisms, individual cells that live independently from each other. Although this is true, microbes in nature mostly live within large multi-species communities forming complex ecosystems. In these communities, microbial cells are held together and organised spatially by an extracellular matrix (ECM). Unlike the ECM from the tissues of higher eukaryotes, microbial ECM, mostly that of yeasts, is still poorly studied. However, microbial biofilms are a serious cause for concern, for being responsible for the development of nosocomial infections by pharmacological drugs-resistant strains of pathogens, or for critically threatening plant health and food security under climate change. Understanding the organization and behaviour of cells in biofilms or other communities is therefore of extreme importance. Within colonies or biofilms, extremely large numbers of individual microbial cells adhere to inert surfaces or living tissues, differentiate, die or multiply and invade adjacent space, often following a 3D architectural programme genetically determined. For all this, cells depend on the production and secretion of ECM, which might, as in higher eukaryotes, actively participate in the regulation of the group behaviour. This work presents an overview of the state-of-the-art on the composition and structure of the ECM produced by yeasts, and the inherent physicochemical properties so often undermined, as well as the available information on its production and delivery pathways.

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Section: Yeasts' Ecm Exopolysaccharidesmentioning

confidence: 99%

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

Lucas¹,

Silva²

2023

Front. Biosci. (Elite Ed)

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“…It is known that the ability to synthesize EPS varies widely both for different EPS producers and for one producer under different conditions of its cultivation (Okoro et al, 2021;Sugumaran et al, 2017). Therefore, for each yeast species, we evaluated the ability to secrete and accumulate EPS in the culture liquid on media with the classical carbon source -glucose and with alternative sources -maltose and mannitol.…”

Section: Variants Of Thementioning

confidence: 99%

“…The ability to synthesize EPS has been found in many microorganisms, but the level of synthesis of these polymers varies widely both for different EPS producers and for one producer under different cultivation conditions (Okoro et al, 2021;Osemwegie et al, 2020;Leroy and Vuyst, 2016). The development of highly efficient technologies for the production of metabolites important for practical use is based on the targeted regulation of the biosynthesis process.…”

Section: Introductionmentioning

confidence: 99%

Accumulation of Exopolysaccharides By Yeasts of Rhodotorula Sp.

Cheban,

Vasina

2023

Biolohichni systemy

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The work is devoted to the search and assessment of the possibility of using alternative carbon sources for the production of exopolysaccharides by yeasts of the genus Rhodotorula sp. Exopolysaccharides (EPS) are high-molecular polymer metabolites of microorganisms produced on the outside of cells. They have a high ability to gel, emulsify, and suspend. The ability to synthesize EPS has been found in many microorganisms, but their level varies widely both for different EPS producers and for one producer under different cultivation conditions. Therefore, the search for active producer strains, alternative nutrient media, and the development of effective microbial exopolysaccharide technologies is an urgent task of biotechnology. The capabilities of three species of the genus Rhodotorula were evaluated: R.rubra, R.minuta, R.glutinis to secrete and accumulate exopolysaccharides (EPS) on classical Sabouraud's medium with glucose was evaluated. The maximum amount of EPS in the culture fluid of R. minuta was determined. Differential diagnostic Hiss media with maltose, lactose and mannitol were used to determine the use of different carbon-containing substrates by yeast cultures. The ability of all three studied yeast species to use maltose and mannitol as a carbon source was established. Accordingly, these substrates were added to the Sabouraud medium in the amount of 20 g/l, 40 g/l or 60 g/l. It was noted that the maximum amount of EPS in the culture liquid of R. rubra and R. minuta accumulates under the conditions of using 60 g/l of mannitol as a carbon source. For R.glutinis, the highest EPS indicator was established on a medium with 60 g/l of glucose.

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“…The search for new biomaterials for tissue engineering, microbial exopolysaccharides (EPSs) have received great attention in recent years. Microbial EPSs such as bacterial Alg are produced by a variety of bacteria and fungi and are involved in a variety of physiological processes, including biofilm construction, nutrition acquisition, stress resistance, and antimicrobial resistance [ 39 , 40 ]. In this particular context, a polyglucuronic acid called glucuronan (PGU), as an EPS produced by the Sinorhizobium meliloti M5N1CS strain, a Gram-negative α-proteobacterium capable of fixing atmospheric nitrogen, described a high molecular weight anionic homopolysaccharide made up of →(4)- β -D-GlcpA-(1)→residue partially O -acetylated at the C-3 and/or the C-2 position [ 10 , 39 , 41 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering

et al. 2022

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3D nanocomposite scaffolds have attracted significant attention in bone tissue engineering applications. In the current study, we fabricated a 3D nanocomposite scaffold based on a bacterial polyglucuronic acid (PGU) and sodium alginate (Alg) composite with carbon nanofibers (CNFs) as the bone tissue engineering scaffold. The CNFs were obtained from electrospun polyacrylonitrile nanofibers through heat treatment. The fabricated CNFs were incorporated into a PGU/Alg polymeric solution, which was physically cross-linked using CaCl2 solution. The fabricated nanocomposites were characterized to evaluate the internal structure, porosity, swelling kinetics, hemocompatibility, and cytocompatibility. The characterizations indicated that the nanocomposites have a porous structure with interconnected pores architecture, proper water absorption, and retention characteristics. The in vitro studies revealed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability assessment showed that the nanocomposites were biocompatible and supported bone cell growth. These results indicated that the fabricated bacterial PGU/Alg/CNFs hydrogel nanocomposite exhibited appropriate properties and can be considered a new biomaterial for bone tissue engineering scaffolds.

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Optimization of Exopolysaccharide (EPS) Production by Rhodotorula mucilaginosa sp. GUMS16

Cited by 18 publications

References 53 publications

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

Accumulation of Exopolysaccharides By Yeasts of Rhodotorula Sp.

Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering

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