Optimization of Extraction Conditions and Validation of the Method for Determination of Arsenic Species in Carrageenan-Producing Seaweed, Kappaphycus spp., Using HPLC-ICP-MS

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…On the other hand, to extract As species (As III , As V , AB, DMA, MMA) from carrageenan-producing seaweed (for which previously published methods were deemed unsuitable) for determination by HPLC-ICP-MS, a method based on heating (at 90 °C) with dilute HNO 3 (0.2%) for 60 min has been devised. 55 Samples were first washed, frozen (−20 °C) for 24 h, freeze dried and ground to a particle size <0.12 mm. The parameters were optimised for both maximum extraction and minimal conversion of As III to As V .…”

Atomic spectrometry update: review of advances in elemental speciation

“…A study describing the optimisation of the extraction conditions for the determination of As III , As V , AB, DMA, and MMA in Kappaphycus alvarezii , a carrageenan-producing red seaweed has been reported. 43 The study used HPLC-ICP-MS to evaluate a series of HNO 3 extraction solutions (0 to 2.0%) with various extraction times (0 to 240 min) at 90 °C. The proposed method was successfully validated using CRM NMIJ 7405-a (Hijiki seaweed) and sample spiking experiments.…”