Optimization of extraction conditions for improving solubility of peanut protein concentrates by response surface methodology

Ma, Tiezheng; Wang, Qiang; Wu, Haoquan

doi:10.1016/j.lwt.2010.03.015

Cited by 50 publications

(42 citation statements)

References 11 publications

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“…As the temperature ascends to 45℃, the protein yield decreases due to the lower solubility of protein stemmed from protein denaturation. Ma et al has also studied the effect of temperature on peanut protein yield and the optimal temperature (36.35℃) was obtained which is close to our result (Ma et al, 2010).…”

Section: Resultssupporting

confidence: 90%

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Jia

et al. 2011

FSTR

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Grape seed is a potential source of edible protein, so effective extraction of protein component from grape seed seems to be very important. However, so far, such information is unavailable. In this study, the conditions for protein extraction from the grape seeds were optimized by the single factor test and the response surface methodology (RSM). A box-behnken design (BBD) was used for experimental design and analysis of the results to obtain the optimal extraction conditions. Solvent/meal ratio, extraction temperature, pH and extraction time were found to have a significant effect on the protein yield. From the single factor test, the optimal range of extraction conditions was obtained. Based on the RSM analysis, optimum extraction parameters were obtained as following: solvent/meal ratio 22.5/1 (v/w), extraction temperature 35℃, pH 9.8, and extraction time 29 min. Under the optimized conditions, the experimental values were in good agreement with those predicted by the model. These results help design the process of optimal protein extraction from grape seeds for future use in food industry.

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Section: Resultssupporting

confidence: 90%

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Jia

et al. 2011

FSTR

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“…Response surface methodology (RSM) is a popular statistical method for optimization of process parameters while conducting the least number of experiments (Firatligil-Durmus and Evranuz, 2010). Protein extraction process optimization using RSM for non-algal sources and Chlorella pyrenoidosa (green algae) was reported previously (Quanhong and Caili, 2005;Zhang et al, 2007;Ma et al, 2010;Wang and Zhang, 2012).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

et al. 2015

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The current work reports protein extraction from Spirulina platensis cyanobacterial biomass in order to simultaneously generate a potential co-product and a biofuel feedstock with reduced nitrogen content. S. platensis cells were subjected to cell disruption by high-pressure homogenization and subsequent protein isolation by solubilization at alkaline pH followed by precipitation at acidic pH. Response surface methodology was used to optimize the process parameters -pH, extraction (solubilization/precipitation) time and biomass concentration for obtaining maximum protein yield. The optimized process conditions were found to be pH 11.38, solubilization time of 35 min and biomass concentration of 3.6% (w/w) solids for the solubilization step, and pH 4.01 and precipitation time of 60 min for the precipitation step. At the optimized conditions, a high protein yield of 60.7% (w/w) was obtained. The protein isolate (co-product) had a higher protein content [80.6% (w/w)], lower ash [1.9% (w/w)] and mineral content and was enriched in essential amino acids, the nutritious γ-linolenic acid and other high-value unsaturated fatty acids compared to the original biomass. The residual biomass obtained after protein extraction had lower nitrogen content and higher total non-protein content than the original biomass. The loss of about 50% of the total lipids from this fraction did not impact its composition significantly owing to the low lipid content of S. platensis (8.03%).

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“…While the extraction temperature was above 49°C, the extraction yield droped rapidly. Ma et al (2010) investigated the effect of temperature on the solubility of peanut protein concentrates in which the increase of temperature above a certain limit resulted in the decrease of protein solubility dramatically, which suggested that proteins are sensitive to high temperatures and a suitable temperature should be selected in extraction of proteins.…”

Section: Validation Of the Modelmentioning

confidence: 99%

Optimization of ultrasound-assisted-extraction of porcine placenta water-soluble proteins and evaluation of the antioxidant activity

2014

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Porcine placenta is commonly used in Chinese as a traditional medicine. It has been reported by a number of researchers that the porcine placenta contains many compounds which have good health benefits. Response surface methodology (RSM) was applied to optimize the parameters of ultrasound power, ultrasound treat time, and extraction temperature on the extraction yield of porcine placenta water-soluble proteins (PPWP). The results indicated that, under optimum conditions of ultrasound power 257 w, extraction temperature at 49°C for 7 min, the extraction yield of PPWP was 32.7 %, which was significantly higher than that of the conventional extraction method (CEM) of 15.0 %. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and the estimated model showed a high capacity of predicting the dependent variables. Although not significantly, the antioxidant activity of PPWP from ultrasound-assist-extraction (UAE) were higher than those from CEM, indicated that UAE had a positive effect or at least no negative effect on the bioactivity of PPWP.

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Optimization of extraction conditions for improving solubility of peanut protein concentrates by response surface methodology

Cited by 50 publications

References 11 publications

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Optimization of Extraction Process of Crude Protein from Grape Seeds by RSM

Optimization of Protein Extraction from Spirulina platensis to Generate a Potential Co-Product and a Biofuel Feedstock with Reduced Nitrogen Content

Optimization of ultrasound-assisted-extraction of porcine placenta water-soluble proteins and evaluation of the antioxidant activity

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