OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROMGENTIANA STRAMINEAMAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC

Abstract

& Extraction time, ratio of methanol aqueous solution to material weight, and methanol concentration for three factor and three level response surface methodology (RSM) was used to study the optimum extraction technology of gentiopicroside from Gentiana Straminea Maxim. The analysis was performed on a high performance liquid chromatography (HPLC) system with gentiopicroside peak areas as response values. A ternary quadratic polynomial model with good correlation (R 2 ¼ 0.9786) was constructed and employed to guide the gentiopicroside extraction process. The optimum extraction conditions were an optimized extract time of 79.4 min, a ratio of methanol aqueous solution to material weight of 23.7 mL=g, methanol concentration of 92.6%, and three times extraction. Under the optimization extraction technology conditions, the predictive value of gentiopicroside peak area of the sample solution was 2120.6, and only 2.03% differ from the mean measured value 2164.6, and the optimized mean extraction yield of gentiopicroside was 78.3 mg Á g À1 . Through solvent distribution method crystallization of the extractive from response surface experiments, the white crystalline compound gentiopicroside was prepared and its purity was greater than 95%.

Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

“…Also, Ariño et al [ 37 ] demonstrated that the content of this secoiridoid compound in yellow gentian root extracts continuously rose with the increase of temperature up to 75 °C. Contrary to our result, Dang et al [ 38 ] reported a significant effect of extraction time on the extraction efficiency of gentiopicroside. Prolonging sonication improved the extraction of gentiopicroside from G. straminea .…”

Development and Validation of a Precise RP- HPLC Method to Determine Gentiopicroside Content in Cultures of Gentiana kurroo Royle

“…R t of 2.5 min was reported for the separation of GPD from G. straminea with a similar mobile phase. 24 In this study, the R t was observed at 9.73 min when 1% acetic acid was added to the mobile phase. The highest peak purity (purity index 1.0) with reasonable resolution and less tailing factor (Figure 3) was achieved with this mobile phase.…”

“…The HPLC method-based determination of GPD was reported previously by a few workers in gentian plants. 16,19,[22][23][24]26,27 However, to date, no single simple method was established and validated for the quantification of this compound in G. kurroo. In this study, we used different mobile phase ratios and flow rates for optimum separation of GPD from different plant material types of G. kurroo to rapidly and accurately quantify the GPD concentration.…”

“…The well dried in vitro and in vivo samples were powdered using a mortar and pestle. The GPD was extracted according to Dang et al 24 with minor modifications. Exactly, 100 mg of fine powder was added to a 2 ml Eppendorf vial containing 1 ml of HPLC grade methanol and sonicated for 20 min at 65°C.…”

“…16,18 The method was also used to compare the relative content of GPD in four Gentiana species, 19,20 (G. macrophylla, G. straminea, G. dahurica and G. crassicaulis) and hairy root cultures of G. macrophylla, 21 G. cruciata L. 22 and G. scabra. 23 Also, GPD content was estimated using HPLC in G. straminea, 24 and in the roots, stems, leaves and flowers of G. rigescens. 25 Although MS/MS and Q-TOF-MS are highly precise and sensitive compared to PDA detectors, they are expensive and operationally tedious.…”