Optimization of extruded collagen fibers for ACL reconstruction

Dunn, Michael G.; Avasarala, Prashanti N.; Zawadsky, Joseph P.

doi:10.1002/jbm.820271211

Cited by 109 publications

(96 citation statements)

References 13 publications

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“…Previously, animal implant studies showed that insoluble collagen threads, crosslinked with a combination of DHT and EDC treatments, facilitated better scaffold degradation and tissue ingrowth properties than either crosslinking technique alone. 46,47 The results of our in vitro studies are consistent with these findings and suggest that the next generation of self-assembled collagen threads should be crosslinked with a physical crosslinking technique such as DHT treatment to mechanically stabilize the scaffolds and EDC crosslinking to facilitate cell migration and new tissue regeneration.…”

Section: Discussionsupporting

confidence: 89%

Crosslinking of discrete self‐assembled collagen threads: Effects on mechanical strength and cell–matrix interactions

Cornwell

Lei

Andreadis

et al. 2006

J Biomedical Materials Res

134

111

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Bundles of threads extruded from type I collagen have been researched extensively as scaffolds to promote the repair and regeneration of torn tendons and ligaments. The success of these scaffolds has been limited by insufficient tissue ingrowth from the wound margin, which may be inhibited by the chemical or physical crosslinking treatment used to increase the mechanical properties and decrease the degradation rate of these scaffolds. Recently, self-assembled collagen threads extruded from solutions of type I collagen molecules were shown to possess ultimate tensile strengths and structural properties comparable to native tendon fibers; however the tissue response to these threads has yet to be determined. The goal of this study was to investigate the effects of various crosslinking techniques on the mechanical properties as well as the in vitro rate of new tissue ingrowth on these threads. Our findings indicate that the physical crosslinking techniques, dehydrothermal (DHT) or ultraviolet light (UV), most significantly improve the mechanical strengths of the threads, but most significantly decrease the rate of cell migration. In contrast, carbodiimide (EDC) crosslinking achieved sub-optimal strength generation, but demonstrated improved cell migration rates. Future studies will investigate the design of threads with surface biochemistries that maximize tissue ingrowth while maintaining the mechanical stability of the scaffold.

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Section: Discussionsupporting

confidence: 89%

Crosslinking of discrete self‐assembled collagen threads: Effects on mechanical strength and cell–matrix interactions

Cornwell

Lei

Andreadis

et al. 2006

J Biomedical Materials Res

134

111

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“…Similar stress-strain curves have also been reported previously for collagenbased biomaterials [57][58][59][60][61][62][63][64] and nano-fibrous meshes [65][66][67]. Most important of all, the electro-spun mats produced in this study were characterised by mechanical properties that closely match those of native tissues, such as anterior cruciate ligament, Achilles tendon and skin and implantable devices [30,62,[68][69][70][71][72][73][74][75].…”

Section: Discussionsupporting

confidence: 83%

Preferential cell response to anisotropic electro-spun fibrous scaffolds under tension-free conditions

English

Azeem

Gaspar

et al. 2011

J Mater Sci: Mater Med

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Anisotropic alignment of collagen fibres in musculoskeletal tissues is responsible for the resistance to mechanical loading, whilst in cornea is responsible for transparency. Herein, we evaluated the response of tenocytes, osteoblasts and corneal fibroblasts to the topographies created through electro-spinning and solvent casting. We also evaluated the influence of topography on mechanical properties. At day 14, human osteoblasts seeded on aligned orientated electro-spun mats exhibited the lowest metabolic activity (P \ 0.001). At day 5 and at day 7, no significant difference was observed in metabolic activity of human corneal fibroblasts and bovine tenocytes respectively seeded on different scaffold conformations (P [ 0.05). Osteoblasts and corneal fibroblasts aligned parallel to the direction of the aligned orientated electro-spun mats, whilst tenocytes aligned perpendicular to the aligned orientated electro-spun mats. Mechanical evaluation demonstrated that aligned orientated electro-spun fibres exhibited significant higher stress at break values than their random aligned counterparts (P \ 0.006) and random orientated electro-spun fibres exhibited significant higher strain at break values than the aligned orientated scaffolds (P \ 0.006). While maintaining fibre structure, we also developed a co-deposition method of spraying and electro-spinning, which enables the incorporation of microspheres within the three-dimensional structure of the scaffold.

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“…Few attempts at creating a bioengineered ACL (bACL) have been reported (Dunn et al 1992(Dunn et al , 1993(Dunn et al , 1994. A drawback of Dunn's approach (Dunn et al 1994) is that the matrix of the graft cannot be quickly degraded, and its remodelling is impaired.…”

Section: Introductionmentioning

confidence: 99%

Torn ACL: A New Bioengineered Substitute Brought from the Laboratory to the Knee Joint

Goulet

Rancourt

Cloutier

et al. 2004

Applied Bionics and Biomechanics

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Anterior cruciate ligament (ACL) injuries occur at an annual rate of 120 000 in the USA, and many need reconstructive surgery. We report successful results at 1-13 months following implantation of bioengineered ACL (bACL) in goats. A bACL has been developed using autologous ACL cells, a collagen matrix and bone plugs. The extremities of the bACL were fully integrated into the femur and tibia of the host. Vascularisation of the grafts was extensive 1 month post-surgery and improved with time. At 6 months postgrafting, histological and ultrastructural observations demonstrated a highly organised ligamentous structure, rich in type I collagen fibres and fibroblasts. At the implants' insertion sites, characteristic fibrocartilage was observed having well aligned chondrocytes and collagen fibrils. After a year, mechanical rupture of the grafts demonstrated a major gain in strength. Eventual applications of this new technology in humans include multiple uses in orthopaedic, dental and reconstructive surgeries.

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Optimization of extruded collagen fibers for ACL reconstruction

Cited by 109 publications

References 13 publications

Crosslinking of discrete self‐assembled collagen threads: Effects on mechanical strength and cell–matrix interactions

Crosslinking of discrete self‐assembled collagen threads: Effects on mechanical strength and cell–matrix interactions

Preferential cell response to anisotropic electro-spun fibrous scaffolds under tension-free conditions

Torn ACL: A New Bioengineered Substitute Brought from the Laboratory to the Knee Joint

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