Optimization of factors influencing microprojectile bombardment-mediated genetic transformation of seed-derived callus and regeneration of transgenic plants in Eleusine coracana (L.) Gaertn

Jagga-Chugh, Swati; Kachhwaha, Sumita; Sharma, Malti; Kothari-Chajer, Aditi; Kothari, S. L.

doi:10.1007/s11240-011-0104-7

Cited by 29 publications

(6 citation statements)

References 34 publications

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“…According to previous literature ( Rasmussen et al, 1994 ; Hilliou et al, 1999 ; Rajasekaran et al, 2000 ; Chong and Maziah, 2005 ; Vongpaseuth et al, 2007 ; Guirimand et al, 2009 ; Xun et al, 2011 ; Jagga-Chugh et al, 2012 ; Rajasekaran, 2013 ; Mousavi et al, 2014 ), the optimization parameters included target tissue distance, helium pressure, chamber vacuum pressure, number of bombardments, precipitation agents, plasmid DNA concentration and post-bombardment incubation time. These parameters and the levels of the variables studied are reported in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Genetic Transformation System for Woody Plant Tripterygium wilfordii and Its Application to Product Natural Celastrol

Zhao

Zhang

et al. 2018

Front. Plant Sci.

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Tripterygium wilfordii is a perennial woody liana medicinal plant with several crucial biological activities. Although studies on tissue culture have previously been conducted, research on genetic transformation is much more challenging and therefore results in slower progress. In the present study, a highly efficient transformation system involving the particle bombardment of T. wilfordii with the reporter egfp gene using the PDS-1000/He system was established. A total of seven parameters affecting the genetic transformation were investigated using an L18 (6 × 36)-type orthogonal array. The result indicated that DNA delivery conditions of 3-cm target distance, 1100 psi helium pressure, 28 mmHg chamber vacuum pressure, three times number of bombardment, CaCl2 as precipitation agent, 2 μg plasmid DNA concentration and 48 h post-bombardment incubation time were optimal for T. wilfordii cell suspensions transformation. The average transformation efficiency was 19.17%. Based on this transformation system, the overexpression of two T. wilfordii farnesyl pyrophosphate synthase genes (TwFPSs) was performed in cell suspensions. Integration of the TwFPSs in the genome was verified by PCR analysis and also by Southern blotting using hygromycin gene as a probe. Real-time quantitative PCR analysis showed that the expression of TwFPS1&2 was highly up regulated in transgenic cell suspensions compared with control cells. The detection of metabolites showed that TwFPS1&2 could highly increase the celastrol content (973.60 μg/g) in transgenic cells. These results indicated that this transformation system is an effective protocol for characterizing the function of genes in the terpenoid biosynthetic pathway.

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Section: Methodsmentioning

confidence: 99%

Genetic Transformation System for Woody Plant Tripterygium wilfordii and Its Application to Product Natural Celastrol

Zhao

Zhang

et al. 2018

Front. Plant Sci.

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“…Progress has also occurred with respect to transgenic protocols for finger millet utilizing Agrobacterium and callus cell bombardment (Kothari et al, 2005 ; Ceasar and Ignacimuthu, 2009 , 2011 ; Sharma et al, 2011 ; Jagga-Chugh et al, 2012 ; Plaza-Wüthrich and Tadele, 2012 ). Such techniques have allowed finger millet plants to be improved for drought and salinity tolerance (Ramegowda et al, 2012 ; Anjaneyulu et al, 2014 ; Hema et al, 2014 ), zinc accumulation (Cakmak, 2008 ; Ramegowda et al, 2013 ), and disease resistance (Latha et al, 2005 ).…”

Section: Diversity Of the Small Milletsmentioning

confidence: 99%

Genetic diversity and genomic resources available for the small millet crops to accelerate a New Green Revolution

2015

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Small millets are nutrient-rich food sources traditionally grown and consumed by subsistence farmers in Asia and Africa. They include finger millet (Eleusine coracana), foxtail millet (Setaria italica), kodo millet (Paspalum scrobiculatum), proso millet (Panicum miliaceum), barnyard millet (Echinochloa spp.), and little millet (Panicum sumatrense). Local farmers value the small millets for their nutritional and health benefits, tolerance to extreme stress including drought, and ability to grow under low nutrient input conditions, ideal in an era of climate change and steadily depleting natural resources. Little scientific attention has been paid to these crops, hence they have been termed “orphan cereals.” Despite this challenge, an advantageous quality of the small millets is that they continue to be grown in remote regions of the world which has preserved their biodiversity, providing breeders with unique alleles for crop improvement. The purpose of this review, first, is to highlight the diverse traits of each small millet species that are valued by farmers and consumers which hold potential for selection, improvement or mechanistic study. For each species, the germplasm, genetic and genomic resources available will then be described as potential tools to exploit this biodiversity. The review will conclude with noting current trends and gaps in the literature and make recommendations on how to better preserve and utilize diversity within these species to accelerate a New Green Revolution for subsistence farmers in Asia and Africa.

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“…The advent of genomics has played a pivotal role in the identification of superior genes for crop improvement, and such approaches are also being tried to identify agriculturally and nutritionally important genes from finger millet. Progress has also occurred with respect to transgenic protocols for finger millet utilizing Agrobacterium and callus cell bombardment approaches (Kothari et al, 2005; Ceasar and Ignacimuthu, 2009, 2011; Sharma et al, 2011; Jagga-Chugh et al, 2012; Plaza-Wüthrich and Tadele, 2012). Latha et al (2005) first developed transgenic finger millet plants expressing pin gene and demonstrated that the expression of fungicidal PIN protein of prawn in the transgenic finger millet bestows high-level of resistance against Pyricularia grisea .…”

Section: Transgenic Developmentmentioning

confidence: 99%

Gene Discovery and Advances in Finger Millet [Eleusine coracana (L.) Gaertn.] Genomics—An Important Nutri-Cereal of Future

et al. 2016

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The rapid strides in molecular marker technologies followed by genomics, and next generation sequencing advancements in three major crops (rice, maize and wheat) of the world have given opportunities for their use in the orphan, but highly valuable future crops, including finger millet [Eleusine coracana (L.) Gaertn.]. Finger millet has many special agronomic and nutritional characteristics, which make it an indispensable crop in arid, semi-arid, hilly and tribal areas of India and Africa. The crop has proven its adaptability in harsh conditions and has shown resilience to climate change. The adaptability traits of finger millet have shown the advantage over major cereal grains under stress conditions, revealing it as a storehouse of important genomic resources for crop improvement. Although new technologies for genomic studies are now available, progress in identifying and tapping these important alleles or genes is lacking. RAPDs were the default choice for genetic diversity studies in the crop until the last decade, but the subsequent development of SSRs and comparative genomics paved the way for the marker assisted selection in finger millet. Resistance gene homologs from NBS-LRR region of finger millet for blast and sequence variants for nutritional traits from other cereals have been developed and used invariably. Population structure analysis studies exhibit 2–4 sub-populations in the finger millet gene pool with separate grouping of Indian and exotic genotypes. Recently, the omics technologies have been efficiently applied to understand the nutritional variation, drought tolerance and gene mining. Progress has also occurred with respect to transgenics development. This review presents the current biotechnological advancements along with research gaps and future perspective of genomic research in finger millet.

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Optimization of factors influencing microprojectile bombardment-mediated genetic transformation of seed-derived callus and regeneration of transgenic plants in Eleusine coracana (L.) Gaertn

Cited by 29 publications

References 34 publications

Genetic Transformation System for Woody Plant Tripterygium wilfordii and Its Application to Product Natural Celastrol

Genetic Transformation System for Woody Plant Tripterygium wilfordii and Its Application to Product Natural Celastrol

Genetic diversity and genomic resources available for the small millet crops to accelerate a New Green Revolution

Gene Discovery and Advances in Finger Millet [Eleusine coracana (L.) Gaertn.] Genomics—An Important Nutri-Cereal of Future

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