Optimization of fermentation conditions and medium compositions for the production of chrysomycin a by a marine-derived strain <i>Streptomyces</i> sp. 891

Ni, Hong-Jin; Lv, Sunyan; Sheng, Ying-Tao; Wang, Hong; Chu, Xiaohe; Zhang, Huawei

doi:10.1080/10826068.2021.1885046

Cited by 29 publications

(14 citation statements)

References 12 publications

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“…Chrysomycin A (Chr-A) ( Figure 1 , C28H28O9 and MW508.52), the major component, differs from chrysomycin B, the second component with the vinyl group of the 8-position [ 5 ], thus showing cytotoxicity towards cancer cells [ 6 , 7 ]. These years, conditions and preparation of Chr-A production has been greatly optimized, which is of great significance to its new drug development process [ 8 , 9 ]. Studies have been done to prove antibiotic activity, anti-tumor activity, anti-tuberculosis activity and anti-neuroinflammation activity of Chr-A [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Chrysomycin A Inhibits the Proliferation, Migration and Invasion of U251 and U87-MG Glioblastoma Cells to Exert Its Anti-Cancer Effects

Liu

Zhang

et al. 2022

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Chrysomycin A (Chr-A), an antibiotic from Streptomyces, is reported to have anti-tumor and anti-tuberculous activities, but its anti-glioblastoma activity and possible mechanism are not clear. Therefore, the current study was to investigate the mechanism of Chr-A against glioblastoma using U251 and U87-MG human cells. CCK8 assays, EdU-DNA synthesis assays and LDH assays were carried out to detect cell viability, proliferation and cytotoxicity of U251 and U87-MG cells, respectively. Transwell assays were performed to detect the invasion and migration abilities of glioblastoma cells. Western blot was used to validate the potential proteins. Chr-A treatment significantly inhibited the growth of glioblastoma cells and weakened the ability of cell migration and invasion by down regulating the expression of slug, MMP2 and MMP9. Furthermore, Chr-A also down regulated Akt, p-Akt, GSK-3β, p-GSK-3β and their downstream proteins, such as β-catenin and c-Myc in human glioblastoma cells. In conclusion, Chr-A may inhibit the proliferation, migration and invasion of glioblastoma cells through the Akt/GSK-3β/β-catenin signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Chrysomycin A Inhibits the Proliferation, Migration and Invasion of U251 and U87-MG Glioblastoma Cells to Exert Its Anti-Cancer Effects

Liu

Zhang

et al. 2022

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“…Chrysomycin A (≥95%) could be obtained by fermentation, subsequent extraction, and purification according to our previous report [ 18 ]. Tefose ® 63 (Mixture of Polyoxyl 6 Stearate Type I, Ethylene Glycol Stearates, and Polyoxyl 32 Stearate Type I) and Labrifil ® M 1944 CS (Oleoyl polyoxyl-6 glycerides) were purchased from GATTEFOSSÉ (Saint Priest, France).…”

Section: Methodsmentioning

confidence: 99%

Formulation of Chrysomycin A Cream for the Treatment of Skin Infections

et al. 2022

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Chrysomycin A, a compound derived from marine microorganisms, proved to have a specific great in vitro inhibitory effect on methicillin-resistant Staphylococcus aureus (MRSA). It exhibits high safety for the skin, as well as a better therapeutic effect than the current clinical drug, vancomycin. Nevertheless, its poor water solubility highly limits the application and reduces the bioavailability. In view of this, we developed a cream of chrysomycin A (CA) to enhance the solubility for the treatment of skin infection, while avoiding the possible toxicity caused by systemic administration. A comprehensive orthogonal evaluation system composed of appearance, spreading ability, and stability was established to find the optimal formula under experimental conditions. The final product was odorless and easy to be spread, with a lustrous, smooth surface. The particle size of the product met Chinese Pharmacopoeia specifications and the entire cream showed long-term stability in destructive tests. The in vitro and in vivo studies indicated that CA cream had a similar anti-MRSA activity to commercially available mupirocin, showing its potential as an efficacious topical delivery system for skin infections treatment.

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“…To obtain the fermentation culture of S. fungicidicus , first, the fermentation seed liquid was prepared according to a previous study [ 48 ]. Then, the seed liquid, after culturing for 18 h, was inoculated into a 2 L Erlenmeyer flask containing 500 mL of MS medium (Sigma-Aldrich, Darmstadt, Germany) at an inoculation amount of 1%.…”

Section: Methodsmentioning

confidence: 99%

Identification of Cyclic Dipeptides and a New Compound (6-(5-Hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one) Produced by Streptomyces fungicidicus against Alternaria solani

et al. 2022

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As an important microbial resource, Actinomycetes, especially Streptomyces, have important application values in medicine and biotechnology. Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato. To obtain pure active compounds, SYH3 fermentation broth was subjected to XAD-16 macroporous resin and silica gel column chromatography. Combined with the repeated preparation and separation of preparative high-performance liquid chromatography (HPLC), a total of four monomer compounds were obtained after activity tracking. Compound 4 was identified as a new six-membered lactone ring compound named 6-(5-hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one by 1D and 2D nuclear magnetic resonance (NMR) data and mass spectrometry (MS). The other three active compounds belong to the cyclodipeptide, and their half maximal inhibitory concentration (IC50) values against A. solani were 43.4, 42.9, and 30.6 μg/mL, respectively. Compound 4 significantly inhibited the spore germination and induced swollen and deformed local hyphae of A. solani with an IC50 value of 24.9 μg/mL. Compound 4 also had broad-spectrum antifungal activity and had a good antifungal effect on the tested plant-pathogenic fungi. The modes of action of new compound (4) still require further investigation, representing a novel and effective anti-fungal agent for future application.

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Optimization of fermentation conditions and medium compositions for the production of chrysomycin a by a marine-derived strain Streptomyces sp. 891

Cited by 29 publications

References 12 publications

Chrysomycin A Inhibits the Proliferation, Migration and Invasion of U251 and U87-MG Glioblastoma Cells to Exert Its Anti-Cancer Effects

Chrysomycin A Inhibits the Proliferation, Migration and Invasion of U251 and U87-MG Glioblastoma Cells to Exert Its Anti-Cancer Effects

Formulation of Chrysomycin A Cream for the Treatment of Skin Infections

Identification of Cyclic Dipeptides and a New Compound (6-(5-Hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one) Produced by Streptomyces fungicidicus against Alternaria solani

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