Optimization of Fermentation Conditions for Pectin Degrading Enzyme Production by Pectinolytic Microbial Consortia Used for Jute Retting

Nath, Minakshi; Majumdar, B.; Das, Suparna; Mazumdar, S.P.; Saha, A. R.; Sarkar, S.

doi:10.20546/ijcmas.2017.611.108

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…Most promising water based microbiological retting process mostly involves bacteria along with various fungi, protozoa, algae and diatoms [10][11][12][13] . Main aerobic retting bacteria belong to genus Bacillus viz., B. subtilis, B. polymyxa, B. mesentericus, B.pumilus, B. cereus, B. megaterium and B. macerans, initiate retting [14][15][16][17][18] along with large numbers of gram-negative genera such as Erwinia and Pseudomonas 9,19 . At the later stage of retting some anaerobic bacteria from genus Clostridium -Clostridium acetobutylicum, Clostridium stercorarium, Clostridium tertium come to carry on the retting process 20 .…”

mentioning

confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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Jute fibre is the second most important fibre next to cotton. It is obtained from the bark of plant through microbial retting process. Here we report optimized microbial retting protocol that can lower retting period and produce high fibre quality. A total of 451 bacterial colonies have been isolated from five jute retting water samples in Bangladesh. Higher pectinolytic bacterial isolates were predominant in the later stage of jute retting. Out of these, 168 isolates have been screened by both semi-quantitative and quantitative pectinase, xylanase and cellulase enzyme assay. Among them, 144 isolates have been selected on the basis of extra cellular enzyme activity of these three enzymes. 16 s ribosomal gene sequencing analysis identified 2 phyla-Firmicutis (80.55%) and Proteobacteria (19.45%). To check the synergistic and antagonistic effect 10 selected isolates were tested in 167 different combinations. Three best combinations were identified that lowered retting period from 18-21 days to 10 days producing high quality fibre in both laboratory and field trial. This improved retting technology can be adopted in industrial scale for the production of quality jute fibre in a controlled condition in reduced water quantity without polluting the environment.Jute, second most important natural fibre after cotton, is cultivated in East Asia and some parts of Latin America 1-3 . Jute is a bast or phloem fibre in the bark of stems, cemented together by pectin and gummy substances 4 . Commercial extraction of jute fibre is water based microbiological retting where jute bundles are submerged into slow running river water and subjected to decomposition of pectin, hemicelluloses, and other mucilaginous substances 2,5 . In this process, pectin is depolymerized by pectinases, primarily comprising four enzymes: Polygalacturonase (PG), Pectin Lyase (PNL), Pectate lyase and Pectin esterase. However, PG 6 , and PNL 7 are primary retting enzymes. In addition, xylanase makes jute fibre softer by selective removal of non-fibrous hemicelluloses without affecting strength of cellulosic fibre. Pectinolytic microorganisms having xylanase activity devoid of cellulase activity is an additional beneficial aspect to improve fibre quality 8 . The quality of fibre is largely determined by the efficiency of retting process 1,2,9,10 and various factors are responsible for proper retting as well as improved fibre quality. Most promising water based microbiological retting process mostly involves bacteria along with various fungi, protozoa, algae and diatoms 10-13 . Main aerobic retting bacteria belong to genus Bacillus viz., B. subtilis, B. polymyxa, B. mesentericus, B.pumilus, B. cereus, B. megaterium and B. macerans, initiate retting 14-18 along with large numbers of gram-negative genera such as Erwinia and Pseudomonas 9,19 . At the later stage of retting some anaerobic bacteria from genus Clostridium -Clostridium acetobutylicum, Clostridium stercorarium, Clostridium tertium come to carry on the retting process 20 . As microorganisms are the ma...

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mentioning

confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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“…The maximum polygalacturonase and pectin lyase enzyme production by pectinolytic microbial consortia could be achieved by using yeast extract pectin media at alkaline pH (8-10). Among the three microbial consortia under study, the polygalacturonase and pectin lyase production in yeast extract pectin agar media by microbial consortium 3 were significantly higher over microbial consortium 1 and 2 (Nath et al 2017).…”

Section: Conventional Whole Plant Rettingmentioning

confidence: 89%

Improved microbial retting and quality jute (Corchorus spp.) fibre production in India – A review

et al. 2022

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Jute (Corchorus spp. L.), the second most important fibre crop next to cotton, is a biodegradable and eco-friendly crop and one of the main cash crops of eastern India. India is the largest producer of raw jute in the world earning about `2200 crores per annum through export of diversified jute goods for which quality jute fibre is needed. The jute fibre quality depends directly on retting process. In India, more than 90% jute farmers do not have the access to free flowing water, so they carry out jute retting in stagnant water. The quality of jute fibre is deteriorated in stagnant water retting because of several factors like less rainfall, repeated retting, absence of efficient retting microbes, direct use of mud, soil etc. In this review paper we have discussed the views of various research workers about the efficient retting microbes, their utilization as microbial consortium for faster retting and quality jute fibre production even in stagnant water retting. Latest molecular approaches for identification of retting microbes including whole genome sequencing of retting microbes and their utilization as talc based formulation, spore based liquid formulation, use of immobilized strain of efficient retting microbes for faster environment friendly jute retting towards quality jute fibre production under farmers’ field conditions have also been discussed in detail.

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“…Among the treatments, the degree of the activity varied, and APPB1 with seawater showed the highest (14.67 U/mL) pectinase activity. The determination of the pectinase enzymatic activity in the degumming cycle indicated the state of pectineus substance degradation, which was positively correlated with retting achievement [53,54]. However, this pectinase activity varied on several factors, including the occurrence of specific bacteria, the maceration effects of the solvents, and their biotic and abiotic factors.…”

Section: Pectinase Enzyme Activitymentioning

confidence: 99%

Augmented Retting Effect on Kenaf Fibers Using Alkalophilic Pectinase-Producing Bacteria in Combination with Water Solvents

2022

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A degumming approach is used in this paper with alkalophilic pectinase-producing bacteria (APPB) and two sources of water solvents to address the existing conventional water retting complexities of kenaf. The incorporation of APPB was confirmed based on their retting feasibilities and multiple cell-wall-degrading enzymatic delicacy. The combinations of APPB with seawater offered retting achievements within six-day retting in non-sterile conditions. These retting niches showed maximum (14.67 U/mL) pectinase activity with fiber separation feasibilities of 4.75 Fried test score. The yielded fiber composition analysis showed a higher cellulose composition (84.65%) and the least amount of hemicellulose, pectin, and ligneous gummy substances. The transmission electron microscopy scan of the yielded fibers showed smooth fiber surfaces, 84.20 µm fiber diameter, and 7.65 g/tex fine fiber compared with uninoculated and combinations of freshwater treatments. The FTIR spectra revealed the cellulosic discrepancies of the retting treatments by monitoring O-H and C=O stretching at ~3300 cm−1 and ~1730 cm−1 wavenumbers. These findings are compelling to yield kenaf fibers of quality considering the existing retting difficulties.

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Optimization of Fermentation Conditions for Pectin Degrading Enzyme Production by Pectinolytic Microbial Consortia Used for Jute Retting

Cited by 4 publications

References 15 publications

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Improved microbial retting and quality jute (Corchorus spp.) fibre production in India – A review

Augmented Retting Effect on Kenaf Fibers Using Alkalophilic Pectinase-Producing Bacteria in Combination with Water Solvents

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