Optimization of Fermentation Medium for Extracellular Lipase Production fromAspergillus nigerUsing Response Surface Methodology

Abstract

Lipase produced by Aspergillus niger is widely used in various industries. In this study, extracellular lipase production from an industrial producing strain of A. niger was improved by medium optimization. The secondary carbon source, nitrogen source, and lipid were found to be the three most influential factors for lipase production by single-factor experiments. According to the statistical approach, the optimum values of three most influential parameters were determined: 10.5 g/L corn starch, 35.4 g/L soybean meal, and 10.9 g/L soybean oil. Using this optimum medium, the best lipase activity was obtained at 2,171 U/mL, which was 16.4% higher than using the initial medium. All these results confirmed the validity of the model. Furthermore, results of the Box-Behnken Design and quadratic models analysis indicated that the carbon to nitrogen (C/N) ratio significantly influenced the enzyme production, which also suggested that more attention should be paid to the C/N ratio for the optimization of enzyme production.

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

“…The F-value of 58.97 indicates that the model is significant and the Lack of Fit (LOF) F-value of 2.56 is insignificant. A coefficient of determination (R 2 ) closer to 1 indicates a better correlation between the predicted and observed values, and a smaller coefficient of variation (C.V.%) implies a higher reliability of the experiment [35,44]. In this study, the R 2 of 0.9823, predicted R of 0.9538 and adjusted R of 0.9826 were consistent, and the predicted R and adjusted R were within 0.20 of each other.…”

“…The main nutrients in the fermentation medium were of carbon (beef extract) and nitrogen (NH 4 NO 3 ) sources. The fermentation biomass depended on the carbon/nitrogen (C/N) ratio and was generally promoted by a higher C/N ratio [34,35]. The experiments showed that Pseudomonas sp.…”

Optimisation of combi‐lipases from Aspergillus niger for the synergistic and efficient hydrolysis of soybean oil

“…AB570425.1, protein molecular weight: 31.57 kDa) from Aspergillus niger strain CICC2475 (Jia et al 2015) Lipase activity was as follows: A, 19 555 U/g; B, 30 429 U/g; C, 21 747 U/g; D, 31 659 U/g. AB570425.1, protein molecular weight: 31.57 kDa) from Aspergillus niger strain CICC2475 (Jia et al 2015) Lipase activity was as follows: A, 19 555 U/g; B, 30 429 U/g; C, 21 747 U/g; D, 31 659 U/g.…”

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

“…It is widely used to identify effective factors, study interactions, select optimum conditions and quantify the relationships between one or more measured responses and the vital input factors in a limited number of experiments [11,30]. Furthermore, RSM has been successfully applied to evaluate and optimize the effect of process parameters in the production of lipase [28,31,32]. A central composite design (CCD) is the most commonly used response surface design when the experimental design is defined by the upper and lower limits of each parameter [16,33].…”