Optimization of fish oil extraction from Lophius litulon liver and fatty acid composition analysis

Abstract

The Lophius litulon liver was used as raw material for the extraction of fish oil via various extraction methods. The extraction rate by water extraction, potassium hydroxide (KOH) hydrolysis and protease hydrolysis were compared and the results revealed the protease hydrolysis extraction had a higher extraction rate with good protein-lipid separation as observed by optical microscope. Furthermore, subsequent experiments determined neutrase to be the best hydrolytic enzyme in terms of extraction rate and cost. The extraction conditions of neutrase hydrolysis were optimized by single-factor experiment and response surface analysis, and the optimal extraction rate was 58.40 ± 0.25% with the following conditions: enzyme concentration 2,000 IU/g, extraction time 1.0 h, liquid-solid ratio 1.95:1, extraction temperature 40.5℃ and pH 6.5. The fatty acids composition in fish oil from optimized extraction condition was composed of 19.75% saturated fatty acids and 80.25% unsaturated fatty acids. The content of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were 8.06% and 1.19%, respectively, with the ratio (6.77:1) surpassed to the recommendation in current researches (5:1). The results in this study suggest protease treatment is an efficient method for high-quality fish oil extraction from Lophius litulon liver with a satisfactory ratio of DHA and EPA.

Packaging films based on biopolymers from seafood processing wastes: Preparation, properties, and their applications for shelf‐life extension of seafoods—A comprehensive review

“…They contain lipids, proteins, and minerals that can be utilized to produce fish oil and fish protein hydrolysates (Nikoo et al., 2022). Fish oil from the head or liver (Baloch et al., 2023; Hu et al., 2022; Vázquez et al., 2022) and shrimp oils from cephalothorax or hepatopancreas (Pascual‐Silva et al., 2022; Raju & Benjakul, 2022) are rich in n − 3 fatty acids, known for their numerous health benefits and can be used as a nutritional supplement. Shrimp oil rich in astaxanthin can serve as an excellent source of natural antioxidants as well as food colorants (Gulzar et al., 2020).…”

Optimization of the Traditional Process of Oil Extraction from Sardine Viscera (Sardina pilchardus) in the Far North Region-Cameroon by the Response Surface Methodology

“…The optimization of the extraction parameters was aimed to maximizing the extraction yield while preserving the qualities of the oil. To meet up with these goals, the criteria set for each response were defined as minimizing the Peroxide value, the Acid value and maximizing the extraction yield based on the example of [18].…”

“…The positive signs of X1 and X3 shows that they contribute positively to improve the yield of fish oil while the negative sign of X2 shows that the proportions of viscera/water could have a negative contribution at a certain extent. All the same, the sign of the quadratic coefficients give the nature of the contributions of various factors on the response [18].…”

“…Table 4 presents the regression coefficients of the response surface models and statistical analysis of each response. The effect of a parameter on the response is consider significant only if the p-values less than 0.05 [18,20,24]. Both regression models have p-values less than 0.05.…”

“…These preliminary results arise from the fact that the extraction processes vary from a producer to the other and the variability of unit operations affects the quality and yield of the fish oil produced [4]. Several authors have solved the fish oil yield extraction problems through optimization, using Response Surface Methods (RSM) [18,19], which appears as a tool to address the glitches faced by the rural communities of the Far North region of Cameroon.…”