Optimization of fractionation efficiency (FE) and throughput (TP) in a large scale splitter less full-feed depletion SPLITT fractionation (Large scale FFD-SF)

Abstract

대용량 splitter less full-feed depletion SPLITT 분획법 (Large scale FFD-SF)에서의 분획효율(FE)및 시료처리량(TP)의 최적화 (2015. 12. 4. 접수, 2015. 12. 11. 수정, 2015. 12. 15. 승인)Abstract: Split-flow thin cell fractionation (SPLITT fractionation, SF) is a particle separation technique that allows continuous (and thus a preparative scale) separation into two subpopulations based on the particle size or the density. In SF, there are two basic performance parameters. One is the throughput (TP), which was defined as the amount of sample that can be processed in a unit time period. Another is the fractionation efficiency (FE), which was defined as the number % of particles that have the size predicted by theory. Full-feed depletion mode (FFD-SF) have only one inlet for the sample feed, and the channel is equipped with a flow stream splitter only at the outlet in SF mode. In conventional FFD-mode, it was difficult to extend channel due to splitter in channel. So, we use large scale splitter-less FFD-SF to increase TP from increase channel scale. In this study, a FFD-SF channel was developed for a large-scale fractionation, which has no flow stream splitters ('splitter less'), and then was tested for optimum TP and FE by varying the sample concentration and the flow rates at the inlet and outlet of the channel. Polyurethane (PU) latex beads having two different size distribution (about 3~7 µm, and about 2~30 µm) were used for the test. The sample concentration was varied from 0.2 to 0.8% (wt/vol). The channel flow rate was varied from 70, 100, 120 and 160 mL/min. The fractionated particles were monitored by optical microscopy (OM). The sample recovery was determined by collecting the particles ★ Corresponding author

Fractionation and characterization of starch granules using field-flow fractionation (FFF) and differential scanning calorimetry (DSC)

“…Thus, the FE values for the fraction-b's were significantly lower than those for the fraction-a's. This was somewhat expected by the absence of inlet-b′ in the FFD mode [11,12,33,34]. In order to increase FE in fraction-b, the collected fractionb was fed again through inlet-a′, yielding Bfraction-ba^and Bfraction-bb.^This was repeated three times in total, yielding Bfraction-bba,^Bfraction-bbb,^Bfraction-bbba,^and Bfraction-bbbb.^At the end, fractions-a, -ba, -bba, and -bbba were mixed together to make Btotal fraction-a.^Table 1 shows the summary of FE and the average size (d avg ) measured for each fraction.…”