Optimization of ?-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose

Abstract

Purified 13-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15 -20 % of the initial substrate concentration. These results suggested that 13-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities.

Efficient synthesis of gluco-oligosaccharides and alkyl-glucosides by transglycosylation activity of β-glucosidase from Sclerotinia sclerotiorum

“…A Maximum GOSs concentration of 112 mg/ml was obtained after 9 h from an initial cellobiose concentration of 350 mg/ ml. Similar findings have previously been described for b-glucosidases from Aspergillus niger (Yan and Liau 1998) and Fusarium oxysporum (Christakopoulos et al 1994). The study of the other reaction-conditions showed, when considering all measured parameters, that the optimum pH was 5 (Fig.…”

Disaccharide Synthesis by Enzymatic Condensation of Glucose: Glycoside Linkage Patterns for Different Fungal Species

“…Transglycosylation is a kinetically controlled reaction, while the reverse hydrolysis is under thermodynamic control. The synthetic activity of glycosidases has been used for production of oligosaccharides [4][5][6][7][8][9][10][11][12], alkyl-glycosides [12][13][14][15][16][17], vitamin derivatives [18]. Oligosaccharides are used as therapeutic agents, diagnostic tools, and additives in the food and cosmetic industries [8,12,19,20].…”

“…The most widely used enzymes for oligosaccharide and alkyl-glycoside synthesis are -glucosidases (EC 3.2.1.21), which act on terminal non-reducing -D-glucosyl residues in cellooligosaccharides and other -D-glucosides with release of -D-glucose [20]. Different -glucosidases from fungi, bacteria and plants have been used in transglycosylation and condensation reactions [4][5][6][7][8][9][10][11][12][13][14][15][16][17][20][21][22][23][24], which are usually carried at high substrate concentration in order to attain higher yields of products. Trisaccharides and higher oligomers are formed as major transglycosylation products of -gluco-*Address correspondence to these authors at the Department of Chemistry, M.V.…”

“…Lomonosov Moscow State University, Moscow 119991, Russia; Tel: +7 495 939 5966; Fax: +7 495 939 0997; E-mails: avgusakov@enzyme.chem.msu.ru; apsinitsyn@enzyme.chem.msu.ru sidase-catalyzed reactions with cellobiose as a substrate [5,6,8,10,12,20,22], while the condensation reactions with glucose as a substrate lead to preferential formation of disaccharides [4,[9][10][11]20]. Although the typical yields of products in the condensation reaction (10-25%) [10,11,20] are usually lower than those obtained with transglycosylation (15-40%) [5,6,10,12,20,22], the substrates (monosaccharides) for condensation are cheaper and the reaction can be more easily controlled. With 90% glucose as a substrate, the unusually high yield of disaccharides (40%) was obtained in reverse hydrolysis reaction catalyzed by almond glucosidase [4], while the yields of several oligosaccharides up to 65% have been achieved by utilizing the transglycosylation activity of Agrobacterium sp.…”

Καθαρισμος Και Μελετη Μιας Β-Γλυκοσιδασης Και Αξιοποιηση Της Στην Υδρολυση Της Λακτοζης

“…Στην υδρόλυση του ß-γλυκανίου συμμετέχουν εκτός από βγλυκοσιδάση, η ένδο-(1-3)-β-0-γλυκανάση και η ß-γλυκανενδοϋδρολάση του κριθαριού. (115,116), ενώ 6-γλυκοσιδάση από Fusarium oxysporum οδήγησε στη σύνδεση τριοζών, σε αντιδράσεις τρανσγλυκοσυλίωσης με υπόστρωμα κελλοβιόζη (117).…”