Optimization of growth and electrosynthesis of PolyHydroxyAlkanoates by the thermophilic bacterium Kyrpidia spormannii

Pillot, Guillaume; Sunny, Soniya; Comes, Victoria; Kerzenmacher, Sven

doi:10.1016/j.biteb.2022.100949

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…Moorella and Thermoanaerobacter species were also enriched in communities on cathodes at 70 • C from anaerobic sludge [14]. Kyrpidia spormannii have been used on cathodes at 60 • C to produce polyhydroxyalkanoates (PHA) from CO 2 [10]. Thus, the present investigation of 11 potential electrotrophs in pure culture significantly expands the panel of known (hyper)thermophilic electrotrophs.…”

Section: Electrotrophy Of (Hyper) Thermophilic Strainsmentioning

confidence: 86%

“…Electrodes were poised at a fixed potential of −600 mV vs. SHE using a potentiostat (IPS Elektroniklabor, PGU-MOD-500mA, Münster, Germany) and the EcmWin software (IPS Elektroniklabor, Münster, Germany). This potential was chosen as it performed best in previous experiments on another thermophilic strain [10]. Chronoamperometry was performed to monitor current consumption at the cathode as a function of time.…”

Section: Microbial Electrochemical Systemmentioning

confidence: 99%

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Screening for Hyperthermophilic Electrotrophs for the Microbial Electrosynthesis of Organic Compounds

et al. 2022

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Microbial electrosynthesis has recently emerged as a promising technology for the sustainable production of organic acids, bioplastics, or biofuels from electricity and CO2. However, the diversity of catalysts and metabolic pathways is limited to mainly mesophilic acetogens or methanogens. Here, eleven hyperthermophilic strains related to Archaeoglobales, Thermococcales, Aquificales, and methanogens were screened for microbial electrosynthesis. The strains were previously isolated from deep-sea hydrothermal vents, where a naturally occurring, spontaneous electrical current can serve as a source of energy for microbial metabolism. After 6 days of incubation in an electrochemical system, all strains showed current consumption, biofilm formation, and small organic molecule production relative to the control. Six selected strains were then incubated over a longer period of time. In the course of one month, a variety of metabolic intermediates of biotechnological relevance such as succinic acid and glycerol accumulated. The production rates and the promotion of specific metabolic pathways seemed to be influenced by the experimental conditions, such as the concentration of CO2 in the gas phase and electron acceptor limitation. Further work is necessary to clearly identify these effects to potentially be able to tune the microbial electrosynthesis of compounds of interest.

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Section: Electrotrophy Of (Hyper) Thermophilic Strainsmentioning

confidence: 86%

Section: Microbial Electrochemical Systemmentioning

confidence: 99%

Screening for Hyperthermophilic Electrotrophs for the Microbial Electrosynthesis of Organic Compounds

et al. 2022

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“…Regarding other genera of this family, there are not any interesting sources indicating their biotechnological or industrial applications. However, Kyrpidia can be considered a polyextremophilic genus that can grow under high acidity and temperature with the ability of polyhydroxyalkanoate (PHA) production [181]. Moreover, there are some reports regarding the Sulfobacillus species that can contribute to chalcopyrite, arsenopyrite, and pyrrhotite bioleaching [182][183][184][185].…”

Section: Alicyclobacillaceae Generamentioning

confidence: 99%

Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives

et al. 2022

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For a long time, the genus Bacillus has been known and considered among the most applicable genera in several fields. Recent taxonomical developments resulted in the identification of more species in Bacillus-related genera, particularly in the order Bacillales (earlier heterotypic synonym: Caryophanales), with potential application for biotechnological and industrial purposes such as biofuels, bioactive agents, biopolymers, and enzymes. Therefore, a thorough understanding of the taxonomy, growth requirements and physiology, genomics, and metabolic pathways in the highly diverse bacterial order, Bacillales, will facilitate a more robust designing and sustainable production of strain lines relevant to a circular economy. This paper is focused principally on less-known genera and their potential in the order Bacillales for promising applications in the industry and addresses the taxonomical complexities of this order. Moreover, it emphasizes the biotechnological usage of some engineered strains of the order Bacillales. The elucidation of novel taxa, their metabolic pathways, and growth conditions would make it possible to drive industrial processes toward an upgraded functionality based on the microbial nature.

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“…While -with respect to the inanimate components of a MES system -much effort has been put into studying the effect of material and structural properties of electrodes, the development of new and, in particular, scalable reactor concepts for MES has been rather sidelined (Enzmann et al, 2018;Guo et al, 2015;Kerzenmacher, 2017;Pillot et al, 2022aPillot et al, , 2022bZhang et al, 2021). This is in spite of the fact that whether microbial electrochemical technologies (METs) can carve a niche in a future sustainable economy depends strongly on the availability of a suitable reactor technology.…”

Section: Introductionmentioning

confidence: 99%

A scalable, rotating disc bioelectrochemical reactor (RDBER) suitable for the cultivation of both cathodic and anodic biofilms

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Gescher

Horn

et al. 2023

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Optimization of growth and electrosynthesis of PolyHydroxyAlkanoates by the thermophilic bacterium Kyrpidia spormannii

Cited by 10 publications

References 33 publications

Screening for Hyperthermophilic Electrotrophs for the Microbial Electrosynthesis of Organic Compounds

Screening for Hyperthermophilic Electrotrophs for the Microbial Electrosynthesis of Organic Compounds

Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives

A scalable, rotating disc bioelectrochemical reactor (RDBER) suitable for the cultivation of both cathodic and anodic biofilms

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