Optimization of HPLC-ICP-AES for the determination of arsenic species

Abstract

High performance liquid chromatography coupled to ICP-AES detection provides a rapid, reliable and sensitive method for arsenic speciation. The separation of As(III), As(V), DMA and MMA was achieved with ion exchange chromatography coupled to an axially-viewed sequential ICP-AES. After optimization of the chromatographic parameters (pH and concentration of the mobile phase), a careful study of the interface was conducted. Five nebulizers associated to three spray chambers were tested. Response of the ICP to each arsenic species was strongly affected by the selection of the nebulizer and spray chamber, however similar responses were obtained for each arsenic species. Best signal-to-noise ratios were obtained by using a microconcentric nebulizer and a cyclone spray chamber and did not affect the chromatographic resolution. Detection limits better than 10 microg L(-1) were obtained for As(III), DMA, MMA and 20 microg L(-1) for As(V), which is a significant improvement over previously published results.

Inductively coupled plasma optical emission spectrometry with axially viewed configuration: an overview of applications

“…93 In addition, the development of a polychromator for ICP OES with axially viewed configuration was also published in the literature. 94 …”

The development and application of hybrid sorbents for determination and selective removal of arsenic(III) and arsenic(V) from water

“…The main advantages of ICP-MS over ICP-AES are isotope analysis capability of high precession and lower detection limits. The results obtained by ICP-AES are comparable to determinations, with low detection limit 0.1 µg/L [21]. However the determination of low concentrations of arsenic in real samples suffers from low sensitivity due to the poor ionization efficiency in ICP.…”

Speciation of arsenic in a thermoacidophilic iron-oxidizing archaeon, Acidianus brierleyi, and its culture medium by inductively coupled plasma–optical emission spectroscopy combined with flow injection pretreatment using an anion-exchange mini-column

“…Ordinarily, speciation is performed by the determination of two of the three As species. Several separation techniques such as high-performance liquid chromatography (HPLC) [3], ion chromatography (IC) [4,5], and capillary electrophoresis [6][7][8] were coupled using either inductively coupled plasma-optical emission spectroscopy (ICP-OES) or inductively coupled plasma-mass spectroscopy (ICP-MS) to achieve a sensitive determination of As(III) and As(V), whereas voltammetry was utilized for the 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 4 when it was used to determine the content of As(III) and As(V) in natural river water.…”