2008
DOI: 10.3855/jidc.208
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Optimization of Human Cytomegalovirus LightCycler Real-Time PCR

Abstract: Background: Real-time PCR has been widely considered as a powerful tool for the evaluation of Human Cytomegalovirus (CMV) DNA kinetics. Successful PCR relies on optimization, which is an extremely demanding procedure. Nevertheless, certain values could be optimal for most primers in use. Methodology: Seventeen CMV primer sets recommended in the literature were selected for optimization in terms of MgCl 2 and primers concentrations as well as annealing temperature using the LightCycler instrument and SYBR Green… Show more

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