Optimization of hydrogen peroxide-to-hemoglobin ratio for biocatalytic mineralization of polycyclic aromatic hydrocarbons (PAHs)-contaminated soils

Analyses of intermediate products during degradation of pyrene in soil by hemoglobin-catalyzed reaction

“…These results indicate that treatment with Hb and H 2 O 2 can be employed to remediate pyrene in soil and possibly other PAHs-contaminated soil. Of note, the catalytic activity of treatment with Hb and H 2 O 2 is due to hemoglobin heme group that reacts with H 2 O 2 to complete the catalytic cycle [10][11][12][13][14].…”

“…It has been commonly used as an indicator to monitor PAHs contaminated soil [7]. Chemical and biological processes or technologies are nowadays preferred means for remediation of PAHs-contaminated sites [8][9][10][11][12].…”

“…Hemoglobin (Hb) can efficiently oxidize organic chemicals such as PAHs. It is known that Hb can acts as a biocatalyst, similar to lignin peroxidases and other heme-containing peroxidases [11][12]. Thus, organic radicals are generated during the catalytic cycle of Hb in the presence of H 2 O 2 .…”

“…Thus, organic radicals are generated during the catalytic cycle of Hb in the presence of H 2 O 2 . These organic radicals might participate in chemical reactions such as oxidation and polymerization, during which various intermediate products can be formed during detoxification of contaminants [10][11][12][13][14][15]. In our recent study with Hb-catalyzed reaction, when pyrene in liquid solution is oxidatively removed, hydrophilic degradation intermediates are formed [15].…”

Hemoglobin–Laccase Modifications of Ligninsulfonate: A Promising Synergistic Stratagem for Lignin Biodegradation

“…[20][21][22] Kang and co-workers reported that hemoglobin promotes peroxide decomposition of recalcitrant organic pollutants like polycyclic aromatic hydrocarbons (PAHs), in which hemoglobin executes a peroxidase role, similar to manganese peroxidase and lignin peroxidase in ligninase. [23][24][25] The heme center converts hydrogen peroxide to hydroxyl radical and compound I (a highly oxidative Fe IV species), leading to a series of radical reactions on the persistent pollutant PAH molecules. [23,26,27] More interestingly, Guillén et al have reported that aryl alcohol oxidase increased production of hydroxyl radical in the laccase reaction, [28] implying that peroxidase synergism enhances formation of highly reactive species in cooperation with laccase.…”