Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production

Abstract

Common carp (Cyprinus carpio) is the fourth most-produced fish species in aquaculture and frequently used model species with significant effort invested in development of biotechnological applications. In present study, we attempted to establish an in vitro germ cell culture condition for short term cell culture, which could facilitate further applications such as surrogacy or gene manipulation. Basal media and different types of feeder cells were investigated to optimize carp germ cell culture condition to favor maintenance of mitotic proliferation. Results indicated that germ cells cultured with hESC media and RTG2 cell line as feeder possessed significantly higher proliferation and survival rate compared to that cultured with StemPro media and Sertoli cell line as feeder. In addition, we compared two dissection strategies to compare risk of cell culture contamination and body cavity was open from dorsal part or from ventral part. As a result, carp open from the dorsal side can minimize the risk of contamination. In summary, this is the first study to optimize the cultivation of germ cells in common carp. This opens up new opportunities for the application of specific techniques in the breeding of those species with high commercial value and frequent use as a model fish. Results obtained in this study are important for implementation of new strategies in common carp breeding, conservation of genetic resources, restoration of lines or development of clonal and isogenic carp lines.

Feeding tea polysaccharides affects lipid metabolism, antioxidant capacity and immunity of common carp (Cyprinus carpio L.)

“…Common carp is one of the most widely cultivated freshwater fish species all over the world, which is the fourth most cultured fish species in aquaculture ( 25 ). The production of common carp accounts for 7.7% of the total fish production in 2018 in the world ( 26 ).…”

“…The production of common carp accounts for 7.7% of the total fish production in 2018 in the world ( 26 ). In addition, the production of common carp reaches 4,411,900 metric tons in 2019 ( 25 ). To meet the needs of human for fish, the intensive high-density and high nutrition farming model was rapidly developed.…”