Optimization of In Vivo Production of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)

Stinguel, Priscila; Paiva, Carlos Eduardo Costa; Zuim, Vitor; Azevedo, Ana Clara Thezolin; Valicente, Fernando Hercos; Júnior, Hugo José Gonçalves dos Santos

doi:10.1007/s13744-021-00917-8

Cited by 3 publications

(7 citation statements)

References 65 publications

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“…It revealed that host plant could play an important role in virus efficacy and demonstrated both horizontal and vertical effects of cannibalism in SfMNPV-KA1 transmission. Developments in in vitro multiplication of baculoviruses have been achieved, yet large-scale production of the viruses as commercial biopesticides has relied on in vivo multiplication in host insects [47]. One of the reasons is the significantly low cost involved and less technology-stringent production requirements for in vivo multiplication.…”

Section: Discussionmentioning

confidence: 99%

Control Potential of Multiple Nucleopolyhedrovirus (SfMNPV) Isolated from Fall Armyworm in Nigeria (West Africa)

Tepa-Yotto,

Douro-Kpindou,

Koussihouédé

et al. 2024

Insects

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The fall armyworm (FAW) Spodoptera frugiperda (Lepidoptera, Noctuidae) has now become an invasive pest of global concern. The pest was first detected in Central and Western Africa in early 2016. Sustainable management options explored by stakeholders during early FAW invasion in Africa included the use of biopesticides and biological control. The current study aimed to compare the susceptibility of FAW larvae to SfMNPV with the assumption that the virus isolated from FAW populations in Africa has higher virulence compared with an isolate from Argentina (SfMNPV-ARG). We also hypothesized that host plant plays a role in SfMNPV efficacy and that cannibalism mediates horizontal and vertical transmission of the virus. This work provides pioneering data on the virulence of the new SfMNPV isolate from Nigeria (SfMNPV-KA1), which proved more effective than its exotic counterpart from Argentina (SfMNPV-ARG). The host plant effect made a significant difference between maize and onion with more FAW death in the larvae fed with contaminated onion 5 days post treatment. The study demonstrates and discusses the effect of cannibalism on virus transmission.

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Section: Discussionmentioning

confidence: 99%

Control Potential of Multiple Nucleopolyhedrovirus (SfMNPV) Isolated from Fall Armyworm in Nigeria (West Africa)

Tepa-Yotto,

Douro-Kpindou,

Koussihouédé

et al. 2024

Insects

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“…The production of S. frugiperda entomopathogenic virus on a commercial scale is in vivo and faces several challenges for successful mass multiplication efficiency, mainly the costs of infrastructure, management, and artificial or natural host diet (ARDISSON-ARAÚJO et al, 2015;SAYED et al, 2021). Therefore the inoculation of viral entomopathogens in in vivo production systems requires host-virus studies, due to the particularities involved in each pathosystem, such as the food substrate and the temperature of viral inoculation, for example (STINGUEL et al, 2022;SUBRAMANIAN et al, 2006;ZAMORA-AVILÉS et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The baculovirus, which has the larvae S. frugiperda as its host, can be inoculated onto the corn or castor bean leaves, or even into an artificial diet identical to that used in mass rearing except for formaldehyde (MACHADO et al, 2021;STINGUEL et al, 2022;TULHER;BARROS, 2010). Inoculation in an artificial diet is done after chilling or pipetting superficially and this poses a challenge to ensure a homogeneous infection of the batches of larvae (ZAMORA-AVILÉS et al, 2017;MACHADO et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…One of the goals of the research is to minimize costs during the production process (MOSCARDI et al, 2011;SOSA-GÓMEZ et al, 2020). Currently, the SfMNPV virus is inoculated into an artificial diet identical to that used in mass rearing the host except for formaldehyde (MACHADO et al, 2021;STINGUEL et al, 2022). However, part of this diet is discarded because the larvae die and/or stop feeding due to the viral infection process.…”

Section: Discussionmentioning

confidence: 99%

“…The efficiency of insect mass rearing, infrastructure costs, management and artificial or natural diet directly affect the cost of production (ARDISSON-ARAÚJO et al, 2015;SAYED et al, 2021). Therefore the inoculation of viral entomopathogens in vivo production systems requires studies focused on the pathogenhost, due to the particularities involved in each pathosystem, such as the food substrate and the temperature of viral inoculation, for example (STINGUEL et al, 2022;SUBRAMANIAN et. al., 2006;ZAMORA-AVILÉS et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Simplified and cool texturing diets for in vivo inoculation of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)

Machado,

Dos Santos Junior,

Paiva

et al. 2023

CLCS

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The inoculation of viral entomopathogens in vivo requires study of each pathosystem. Several factors interfere in the cost of production and consequently in the availability of virus-based formulations. For the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), employed in the management of the fall armyworm Spodoptera frugiperda, factors such as host cannibal habit, temperature, and artificial diet of viral inoculation are important to research. In this work we propose to evaluate a diet based on textured soy protein, without heating during preparation, for viral inoculation and an artificial diet of simplified composition, based on wheat germ, to complete the feeding of larvaes during the stage of viral inoculation in groups of insects. S. frugiperda larvae were inoculated with SfMNPV virus in two diets: an artificial diet similar to that of mass rearing of the host and texturized soy protein at the following temperatures and respective ages: at 25 °C at 6 and 8 days after hatching and 31 °C at 4 and 6 days after larvae hatching. And, subsequently, viral inoculation was evaluated in a group of 300 larvae under different incubation times and temperature conditions, complementing feeding at this stage with a diet of simplified composition. The results show that it is possible to inoculate viruses without loss of viral polyhedra production in a textured soy protein-based diet and supplement the larvae diet with an artificial diet of simplified composition to complete the viral infection stage in groups of infected insects.

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Mass Breeding of Phytophagous Insects Using Biologically Active Substances

Agas'eva,

Nefedova,

Nastasiy

2024

Bulletin of KSAU

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The purpose of the study is to develop a method to increase the efficiency of the production of host insects for the mass breeding of entomophages. Mass cultivation of cereal aphids includes sowing seeds of wheat or barley, settling aphids in plants, collecting and counting aphids. To identify plant growth regulators (PPR) that stimulate the output (yield) of cereal aphids during its cultivation, the following preparations were tested: Immunocytofit (Tablets), Albit (fluid paste), Chitosan Bio (wettable soluble powder), Radipharm (liquid), Hydromix (microgranules), Kendal (solution), Biodux (liquid), Zircon (solution), Krasnodar-1 (solution). The experiment was established by pre-sowing seed treatment. Three days after germination, the wheat was infested with aphids, the effectiveness was determined by weighing the aphid biomass. The effectiveness of the preparations was determined by weighing the biomass of aphids before infection and 9 days after infection in comparison with the control variant. We studied the possibility of growing entomophages of harmony variable (Harmonia axyridis Pallas) and the aphid parasite Aphidius chamomile (Aphidius matricariae Hal.) It has been established that the use of plant growth regulators Immunocytofit (Tablets), Biodux (liquid), Chitosan Bio (wettable soluble powder) and Krasnodar-1 (solution), significantly increases aphid biomass (almost 4 times in all cases compared with control: about 550 mg is the average value of the biomass of aphids grown using the preparations Immunocytofit (Tablets), Chitosan Bio (wettable soluble powder), Biodux, (liquid), and Krasnodar-1 against 140 mg in control) and does not adversely affect the process of breeding aphidophages. The rapid increase in the number of aphids is promising for the technologies of mass breeding of phytophagous insects in the programs of biological plant protection.

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Optimization of In Vivo Production of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)

Cited by 3 publications

References 65 publications

Control Potential of Multiple Nucleopolyhedrovirus (SfMNPV) Isolated from Fall Armyworm in Nigeria (West Africa)

Control Potential of Multiple Nucleopolyhedrovirus (SfMNPV) Isolated from Fall Armyworm in Nigeria (West Africa)

Simplified and cool texturing diets for in vivo inoculation of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)

Mass Breeding of Phytophagous Insects Using Biologically Active Substances

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