Optimization of Inulin Hydrolysis by Penicillium lanosocoeruleum Inulinases and Efficient Conversion Into Polyhydroxyalkanoates

Corrado, Iolanda; Cascelli, Nicoletta; Ntasi, Georgia; Birolo, Leila; Sannia, Giovanni; Pezzella, Cinzia

doi:10.3389/fbioe.2021.616908

Cited by 15 publications

(14 citation statements)

References 76 publications

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“…For copolymer poly-(3 hydroxybutyrate)-co-(3-Hydroxyhexanoate) (PHB-HHx) production, recombinant Escherichia coli strain, LipoB, was cultured in an Eppendorf NewBrunswick, BioFlo/CelliGen ® 115 bioreactor according to Corrado et al [ 23 ]. Polymers were recovered according to Corrado et al [ 26 ] and Corrado et al [ 23 ] for PHB and PHB-HHx, respectively.…”

Section: Methodsmentioning

confidence: 99%

Polyhydroxyalkanoates-Based Nanoparticles as Essential Oil Carriers

et al. 2022

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Plant-derived essential oils (EOs) represent a green alternative to conventional antimicrobial agents in food preservation. Due to their volatility and instability, their application is dependent on the development of efficient encapsulation strategies allowing their protection and release control. Encapsulation in Polyhydroxyalkanoate (PHA)-based nanoparticles (NPs) addresses this challenge, providing a biodegradable and biobased material whose delivery properties can be tuned by varying polymer composition. In this work, EO from Mexican oregano was efficiently encapsulated in Polyhydroxybutyrate (PHB) and Poly-3-hydroxybutyrate-co-hydroxyhexanoate (PHB-HHx)-based NPs by solvent evaporation technique achieving high encapsulation efficiency, (>60%) and loading capacity, (about 50%). The obtained NPs displayed a regular distribution with a size range of 150–210 nm. In vitro release studies in food simulant media were fitted with the Korsmeyer–Peppas model, indicating diffusion as the main factor controlling the release. The cumulative release was affected by the polymer composition, possibly related to the more amorphous nature of the copolymer, as confirmed by WAXS and DSC analyses. Both the EO-loaded nanosystems displayed antimicrobial activity against Micrococcus luteus, with PHB-HHx-based NPs being even more effective than the pure EO. The results open the way to the effective exploitation of the developed nanosystems in active packaging.

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Section: Methodsmentioning

confidence: 99%

Polyhydroxyalkanoates-Based Nanoparticles as Essential Oil Carriers

et al. 2022

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“…The biorefinery concept focuses on the sustainable conversion of biomass into a wide range of products, materials, and energy [ 17 ]; polymer matrices and fibers or fillers are some of such products that can be obtained from a biorefinery.…”

Section: Polymer Compositesmentioning

confidence: 99%

“…Polyhydroxyalkanoates (PHA) are a promising alternative to conventional polymers, as they are fully biodegradable and are also biobased (obtained from bacteria), and show a wide range of properties, similar to polyolefins, depending on their molecular weight [ 17 ]. In this sense, Corrado [ 17 ] proposes the use of an inulin-—rich biomass (from plants roots) and a microbial fermentation, obtaining high polymer yields, while Kumar also obtained polyhydroxybutirate (PHB) from vegetal wastes, and reinforce the matrix with cellulose also coming from wastes (sugarcane bagasse in this case) [ 20 ]. Rigid polyurethane foams can also be synthesized from waste vegetable oils, through a transesterification reaction [ 21 , 22 ].…”

Section: Polymer Compositesmentioning

confidence: 99%

Are Natural-Based Composites Sustainable?

et al. 2021

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This paper assesses the aspects related to sustainability of polymer composites, focusing on the two main components of a composite, the matrix and the reinforcement/filler. Most studies analyzed deals with the assessment of the composite performance, but not much attention has been paid to the life cycle assessment (LCA), biodegradation or recyclability of these materials, even in those papers containing the terms “sustainable” (or its derivate words), “green” or “eco”. Many papers claim about the sustainable or renewable character of natural fiber composites, although, again, analysis about recyclability, biodegradation or carbon footprint determination of these materials have not been studied in detail. More studies focusing on the assessment of these composites are needed in order to clarify their potential environmental benefits when compared to other types of composites, which include compounds not obtained from biological resources. LCA methodology has only been applied to some case studies, finding enhanced environmental behavior for natural fiber composites when compared to synthetic ones, also showing the potential benefits of using recycled carbon or glass fibers. Biodegradable composites are considered of lesser interest to recyclable ones, as they allow for a higher profitability of the resources. Finally, it is interesting to highlight the enormous potential of waste as raw material for composite production, both for the matrix and the filler/reinforcement; these have two main benefits: no resources are used for their growth (in the case of biological materials), and fewer residues need to be disposed.

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“…JA can grow on different soils and in different climates, from dry deserts to frost-prone regions, unsuitable for growing food crops such as wheat, rice, and potato [ 36 ]. The tubers of JA contain a unique carbohydrate group based on fructose and its polymers, whose higher homologue is inulin [ 37 , 38 , 39 ]. Inulin is enzymatically hydrolyzed to fructose and glucose due to the combined action of inulinase and invertase [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

“…The inulin content of the tubers constitutes 70–80% of the total dry weight [ 41 , 42 ]. Inulin mainly consists of fructose (85–95%), and fructose content of JA varies depending on the harvesting dates, storage duration, etc., as it is the product of inulin metabolism in JA roots and tubers [ 39 ]. JA tubers also contain large amounts of inulids, which are depolymerized to inulin by the enzymes.…”

Section: Introductionmentioning

confidence: 99%

Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass

et al. 2021

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One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains—C. necator B-10646 and R. eutropha B 5786 and B 8562—were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 °C followed by acid hydrolysis at 60 °C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100–120 °C difference between the Tmelt and Tdegr, prevailing of the crystalline phase over the amorphous one (Cx between 69 and 75%), and variations in weight average molecular weight (409–480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and ε-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology—finding widely available and inexpensive substrates.

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Optimization of Inulin Hydrolysis by Penicillium lanosocoeruleum Inulinases and Efficient Conversion Into Polyhydroxyalkanoates

Abstract: Graphical AbstractScheme of the developed bioprocesses.

Cited by 15 publications

References 76 publications

Polyhydroxyalkanoates-Based Nanoparticles as Essential Oil Carriers

Polyhydroxyalkanoates-Based Nanoparticles as Essential Oil Carriers

Are Natural-Based Composites Sustainable?

Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass

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