Optimization of inulinase production by solid-state fermentation using sugarcane bagasse as substrate

Mazutti, Márcio A.; Bender, João Paulo; Treichel, Helen; Luccio, Marco Di

doi:10.1016/j.enzmictec.2005.09.008

Cited by 98 publications

(68 citation statements)

References 16 publications

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“…Other authors have also reported similar optimum pH and temperature values for K. marxianus [38,39,46,49], but inulinases from other fungi species have also presented similar biochemical characteristics [17,26,50]. This characteristic is very interesting for the industrial sector, because acid pH and higher temperatures decrease the risk of contamination [16].…”

Section: Discussionmentioning

confidence: 61%

“…Furthermore, the complex composition of agroindustrial substrates can cause a negative effect due to the increase in the concentration of some of their constituents [43]. Regarding corn steep liquor, different concentrations are found in the literature, ranging from 0.3% to 20%, or even more [29,45,46,47,48] showing that the response to this substrate depends mainly on the microorganism used and should be adjusted to the metabolic needs of each one.…”

Section: Discussionmentioning

confidence: 99%

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Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

Sathler

Contiero

Cazetta

2014

JBT

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Yacon is a perennial plant native of the Andes that stores inulin-type polyfructans as reserve carbohydrates in its tubers. The enzymes that hydrolyze the inulin β-2,1 fructose linkages are called inulinases and are much used to produce high fructose syrups (HFS) and ethanol. The present study investigated inulinase and biomass production by Kluyveromyces marxianus NRRL Y-7571 using yacon flour as carbon source and corn steep liquor as nitrogen source by a Central Composite Design (CCD) 2 2 to carry out experiments and Surface Response Methodology (RSM) to analyze the results. The maximum enzyme production was 36.1 U.ml -1 and biomass production was 8.95 g.l -1 under the condition of 7% yacon flour and 2% corn steep liquor. The crude enzyme partial characterization showed that inulinase had optimal pH at 4.0 and optimal temperature at 60°C. The enzyme maintained over 90% of its activity for four hours at 45°C and 50°C.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

Sathler

Contiero

Cazetta

2014

JBT

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Section: Resultsmentioning

confidence: 99%

“…Temperature values higher than 30ºC may lead to enzymatic inactivation (Mazutti et al, 2006) or suppression of cell viability (Francis et al, 2002). In contrast, low temperature values may reduce the metabolism of the microorganism (Mazutti et al, 2006) and consequently, the enzyme synthesis. In this study, these results of present study suggest that Tapioca yielded highest amylase activity at 50% moisture and it was also verified that A. niger has a preference for acidic (pH 5) and moderate temperature (30°C) probably as a for growth and extracellular amylase production.…”

Section: Resultsmentioning

confidence: 99%

Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by <i>Aspergillus niger</i>

Dharani¹,

Kumaran

2012

Bangladesh J. Sci. Ind. Res.

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The purpose of this work is to study the optimized cultural conditions for the production of amylase by Aspergillus niger in solid state and submerged liquid fermentation. Four solid substrates banana peel, corn, potato and tapioca with different moisture conditions were taken for solid state fermentation (SSF). Basal medium was used for submerged liquid fermentation (SLF) with different pH (3 to 8), temperature (25, 30, 35 and 40ºC), carbon concentration (1, 2 and 3 g) and nitrogen source (0.1, 0.2 and 0.3 g). In SSF, tapioca yielded highest amylase activity and specific activity (4.43U/ml and 4.58U/mg) at 50% moisture content. In SLF, 2 g starch and 0.3 g peptone concentration showed 0.78 and 1.23 U/ml amylase activities under the optimum pH (5) and temperature (30ºC) the amylase activities reached to 0.86 U/ml and 0.76 U/ml respectively. In SSF using tapioca as substrate the enzyme yield is about five times higher than that achieved with submerged liquid culture.

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“…Solid-state fermentation (SSF) may be defined as a fermentation process where the microorganisms grow in solid substrates with low water concentration. Many studies on the application of SSF are focused in adding value to agroindustry residues, which have been extensively used as a physical support or source of nutrients in SSF (Mazutti et al 2006). The enzymes produced by fermentation processes offer a number of advantages and have been widely researched and used throughout the world.…”

Section: Introductionmentioning

confidence: 99%

Partial characterization of an inulinase produced by Aspergillus japonicus URM5633

Silva

Queiroz

Porto

et al. 2012

Braz. arch. biol. technol.

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Optimization of inulinase production by solid-state fermentation using sugarcane bagasse as substrate

Cited by 98 publications

References 16 publications

Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by <i>Aspergillus niger</i>

Partial characterization of an inulinase produced by Aspergillus japonicus URM5633

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