Optimization of inulinase production from low cost substrates using Plackett–Burman and Taguchi methods

Aty, Abeer A. Abd El; Wehaidy, Hala R.; Mostafa, Faten A.

doi:10.1016/j.carbpol.2013.11.007

Cited by 62 publications

(22 citation statements)

References 26 publications

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“…Some previous studies suggested that inulinase was produced by different microorganisms using different carbon sources which support the present study (Abd El Aty et al, 2014;Dilipkumar et al, 2014;Treichel et al, 2009;Trivedi et al, 2012).…”

Section: Optimization Of Factors By Response Surface Methodology (Rsm)supporting

confidence: 87%

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Rawat

Soni

Kango

et al. 2017

Biocatalysis and Agricultural Biotechnology

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Section: Optimization Of Factors By Response Surface Methodology (Rsm)supporting

confidence: 87%

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Rawat

Soni

Kango

et al. 2017

Biocatalysis and Agricultural Biotechnology

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“…The suspension was filtered through a nylon cloth, followed by centrifugation at 5000 rpm for 15 min, 4 °C. The filtrate obtained was used for determination of naringinase activity (Abd El Aty et al 2014; Faten and Abeer 2013)…”

Section: Methodsmentioning

confidence: 99%

Covalent immobilization of microbial naringinase using novel thermally stable biopolymer for hydrolysis of naringin

et al. 2016

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Naringinase induced from the fermented broth of marine-derived fungus Aspergillus niger was immobilized into grafted gel beads, to obtain biocatalytically active beads. The support for enzyme immobilization was characterized by ART-FTIR and TGA techniques. TGA revealed a significant improvement in the grafted gel’s thermal stability from 200 to 300 °C. Optimization of the enzyme loading capacity increased gradually by 28-fold from 32 U/g gel to 899 U/g gel beads, retaining 99 % of the enzyme immobilization efficiency and 88 % of the immobilization yield. The immobilization process highly improved the enzyme’s thermal stability from 50 to 70 °C, which is favored in food industries, and reusability test retained 100 % of the immobilized enzyme activity after 20 cycles. These results are very useful on the marketing and industrial levels.

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“…; El Aty et al . ), in which six factors (temperature, material to liquid ratio, duration, extraction times, ultrasonic power and modulation frequency) were included and evaluated using the SAS version 8.2 software (SAS Institute, Inc., Cary, NC). Each factor (independent variable) was tested at two levels: high (+1) and low (−1).…”

Section: Methodsmentioning

confidence: 99%

“…According to our preliminary experimental results, the yield of carotenoids was significantly influenced by temperature, material to liquid ratio, duration, extraction times, ultrasonic power and modulation frequency (data not shown), and their ranges between the model levels described as −1 and 1 were selected and evaluated for optimization of carotenoid extraction: ultrasonic power (A, 120-180 W); modulation frequency (B, 40-100 Hz); duration (C, 10-45 min); temperature (D, 20-50C), to minimize the solvent loss, and the ground flask was accompanied with reflux condense); liquid to material ratio (mL/g), E (15-30) and extraction times, F (1-3) were evaluated. The following experiments were designed with Plackett-Burman (PBD) method (Zhou et al 2009;El Aty et al 2014), in which six factors (temperature, material to liquid ratio, duration, extraction times, ultrasonic power and modulation frequency) were included and evaluated using the SAS version 8.2 software (SAS Institute, Inc., Cary, NC). Each factor (independent variable) was tested at two levels: high (+1) and low (−1).…”

Section: Plackett-burman Experimental Designmentioning

confidence: 99%

Ultrasonic‐Assisted Solvent Extraction of Carotenoids From Rapeseed Meal: Optimization Using Response Surface Methodology

Yan

Fan

et al. 2015

Journal of Food Quality

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Ultrasound-assisted solvent extraction of carotenoids from rapeseed meal was investigated and evaluated by response surface methodology. We have compared the yields of carotenoids by using five different solvent systems, and found that the highest yield can be achieved under petroleum ether/acetone (v/v = 1/1). We have further tested the effect of ultrasonic power, modulation frequency, duration, temperature, liquid to material ratio and extraction times on the yield of carotenoids by adopting Plackett-Burman and Box-Behnken experimental design methods. Optimal conditions for obtaining high yield of carotenoids were as follows: temperature 49.6C, liquid to material ratio 41.4 mL/g, duration 48.5 min, ultrasonic power 252.9 W (240 W was used in the control experiments). Under the above conditions, average carotenoid yield was 0.1577 ± 0.0014 mg/g, and the extraction percentage was 79.61 ± 0.71%. The results indicated that rapeseed meal was a promising alternative source for carotenoid extraction. PRACTICAL APPLICATIONSCarotenoids are one of the most common natural food colorants and exist widely in plants, bacteria, fungi and animals. Ultrasound has been utilized to assist the solvent extraction of bioactive compounds from vegetal materials, and led to high yield of desired products. In the present study, we have investigated ultrasoundassisted extraction of carotenoids from rapeseed meal using the response surface methodology. In order to obtain the highest yield of carotenoids, we have examined various operational parameters, such as extraction temperature, liquid to material ratio, solvent composition, extraction time and modulation frequency, and have established optimal conditions to extract carotenoids from rapeseed meal. Our results provide basic knowledge on the extraction of carotenoids from rapeseed meal, which serves as a promising bioresource.

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Optimization of inulinase production from low cost substrates using Plackett–Burman and Taguchi methods

Cited by 62 publications

References 26 publications

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Covalent immobilization of microbial naringinase using novel thermally stable biopolymer for hydrolysis of naringin

Ultrasonic‐Assisted Solvent Extraction of Carotenoids From Rapeseed Meal: Optimization Using Response Surface Methodology

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