Optimization of invertase immobilization by adsorption in ionic exchange resin for sucrose hydrolysis

Reversible immobilization of Candida rugosa lipase on fibrous polymer grafted and sulfonated p(HEMA/EGDMA) beads

“…For example, cation-exchange groups modified fibrous polymer brushes can interact through the amino groups and hydrogen donor sites of the adsorbed enzyme under mild experimental conditions. This technique may be a good option, because adsorption is very simple and the method needs very little work and time [27,28]. In addition, the supports can be reused after desorption of the inactivated enzyme upon use.…”

Invert sugar formation with Saccharomyces cerevisiae cells encapsulated in magnetically responsive alginate microparticles

“…In the case of 30% sucrose the conversion was 36.7% and 82.2% after 30 and 90 min, respectively. Increased sucrose concentrations (40% and 50%) caused its lower conversion to glucose and fructose (55.9% and 31.4%) by the prepared biocatalyst during 90 min incubation; similar behavior (the substrate inhibition kinetics) was observed for both free and immobilized invertase [2].…”

“…Invert sugar is used mainly by food manufacturers to retard the crystallization of sugar in highviscosity solutions [1]. It is used in the production of noncrystallizing creams, jams, artificial honey, and in confectionery industry and to a lesser extent in the industrial production of liquid sugar [2,3].…”

“…The enzyme is usually obtained from baker's yeasts (Saccharomyces cerevisiae). The isolated enzyme can be used for sucrose hydrolysis both in the free and immobilized form [2,5]; however, the isolation process increases the cost of purified enzymes.…”

Immobilization of Invertase in Copolymer of 2,5-Di(thiophen-2-yl)-1-p-Tolyl-1H-Pyrrole with Pyrrole

“…In addition, soluble invertase is used in the production of artificial honey, jam and to a small extent liquid sugar in the sweetener industry (8)(9)(10). Immobilization of invertase on corn grifts (11), polyethylene (12), polyaniline (13), gelatin (14), carbohydrate moieties (15), polyelectrolytes (16), porous cellulose beads (17), diazonium salt of 4-amino-benzoylcellulose (18), and poly (ethylene-vinylalcohol) (19) has been studied.…”