Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

Abstract

Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.

Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

“…In a similar study, Lo et al [26] reported the overwhelming effect of tryptone on the lipase activity from Burkholderia specie. Tryptone is seldom reported as optimum organic nitrogen source, only other reported [25,31].…”

“…Lipase activity of lipase obtained from Burkholderia sp. in the presence of olive oil and at 30°C was 122.3 U/mL [26]. Rajendran et al [27] identified a strain of Bacillus brevis capable to secrete alkaline lipase and its lipase activity increased to 5.1 U/mL after optimization of the culture medium.…”

Enhancing Extracellular Lipolytic Enzyme Production In An Arctic Bacterium,Psychrobactersp. ArcL13, By Using Statistical Optimization And Fed-Batch Fermentation

“…We obtained 10-fold higher enzyme activity by such optimization. Previously, statistical optimization studies have been carried out using Moritella sp., [17] Burkholeria sp., [16] Pseudomonas sp., [29] and Geotrichum sp. [30] Overall, 2.6-to 6.0-fold increases in lipolytic enzyme production were obtained compared to that observed before optimization.…”

“…[25][26][27][28] The medium components affecting the production of lipolytic enzyme were optimized using statistical design. There have been several reports that some complex nitrogen sources stimulate lipase production: soybean meal, [17] tryptone, [16] yeast extract, [25] and peptone. [27] In our case, yeast extract showed the most positive effect on lipolytic enzyme production.…”

“…However, statistical experimental design, including two-factorial design and response surface methodology, has proven effective for the optimization of culture medium. [15][16][17][18][19] Therefore, statistical experimental design was carried out to optimize the medium components. This study describes a statistical approach and fed-batch fermentation to optimize and enhance the production of lipolytic activity in bacterial strains isolated from the Arctic Sea.…”