Optimization of medicinal plant extraction methods and their encapsulation through extrusion technology

Rijo, Patrícia; Falé, Pedro L.; Serralheiro, Maria Luísa; Simões, M. Fátima; Gomes, Ana; Reis, Catarina Pinto

doi:10.1016/j.measurement.2014.08.045

Cited by 49 publications

(49 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Adicionaram-se 30 mL de acetona a 3 g de folhas trituradas de planta seca, três vezes durante 1 hora cada extração, e filtrou-se usando filtros Whatman papel Nº 5 (Whatman, Inc., Clifton, NJ, USA.) (20) . Os extratos obtidos foram evaporados usando o evaporador rotativo a 40-50 o C. O extratos foram depois colocados em Eppendorfs e dissolvidos em DMSO a uma concentração de 10 mg/mL.…”

Section: Preparação Dos Extratosunclassified

“…Acetylcholinesterase enzymatic activity was evaluated in accordance with the method described by Rijo et al, (20) using a microplate reader. All tests were carried out in triplicate with tacrine as the positive control.…”

Section: In Vitro Acetylcholinesterase Inhibitionmentioning

confidence: 99%

“…A atividade enzimática da acetilcolinesterase foi avaliada de acordo com o método descrito por Rijo et al, (20) usando um leitor de microplacas (Secção 2.2).Todos os testes foram executados em triplicado usando tacrina como controlo positivo. Os extratos das plantas foram testados a uma concentração de 0,1 µg/mL de acordo com as equações em Equação 2 e 3.…”

Section: Atividade Da Acetilcolinesterase In Vitrounclassified

“…The antioxidant activity of all extracts was measured by DPPH method, as described by Rijo et al (20) 10 µL of each extract sample were added to a 990 µL solution of DPPH (0.002% in methanol). The mixture was incubated for 30 minutes at room temperature.…”

Section: Determination Of Antioxidant Activity (Dpph)mentioning

confidence: 99%

“…Para avaliar a capacidade de recaptação de radicais do extrato, foi usado o método do DPPH (20) . Foram adicionados 10 µL de extrato de planta (amostra) a 990 μL de solução de DPPH (0,002% em metanol).…”

Section: Determinação Da Atividade Antioxidante (Dpph)unclassified

See 4 more Smart Citations

Biological activity screening of seven Plectranthus species

Ntungwe

Marçalo²,

García³

et al. 2017

BBR

Self Cite

View full text Add to dashboard Cite

Biopharmaceutical Sciences│Ciências BiofarmacêuticasBiomed Biopharm Res. , 2017; (14) 1: , 95-108 95 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c a Biological activity screening of seven Plectranthus species AbstractNatural products from Plectranthus spp. plants have an ethnopharmacological use, inspiring several scientific investigations. As such, this work aims to perform a biological activity screening in order to scientifically validate the use of these plants. Assays on in vitro acetylcholinesterase (AChE) inhibition, antioxidant effects, antimicrobial activity and Artemia salina lethality were performed on seven Plectranthus spp. extracts (P. swynnertonii, P. welwischii, P. woodii, P. cylindraceus, P. spicatus, P. ramosior and P. petiolaris). Acetonic extracts were obtained by sonication (10% w/v), where P. ramosior had the highest yield of dry extract (13.49% w/w). In the AChE inhibition assay, only P. cylindraceus extract decreased enzymatic activity (30.2 ± 3.78%). The antimicrobial activity was screened using the well diffusion method, against Gram positive and negative bacteria and yeast. P. ramosior extract showed not only an inhibition zone against S. aureus and C. albicans (15 and 11 mm, respectively), but also the highest scavenging activity (DPPH method, 36.4 ± 0.04%). On the lethality test in A. salina, P. swynnertonnii extract was the most toxic (LC50 = 0.036 mg/L). These preliminary results showed that P. cylindraceus, P. ramosior and P. swynnertonnii are potential bioactive extracts for further isolation and antimicrobial and cytotoxic studies.Keywords: Plectranthus, Acetylcholinesterase, Antioxidant, Antimicrobial, Toxicity ResumoOs produtos naturais das espécies de plantas Plectranthus têm um uso etnofarmacológico, suscitando vários estudos científicos. Neste trabalho pretendeu-se validar cientificamente estas plantas, realizando uma pesquisa de atividades biológicas. Extratos de sete espécies de Plectranthus (P. swynnertonii, P. welwischii, P. woodii, P. cylindraceus, P. spicatus, P. ramosior e P. petiolaris) foram testados na inibição da acetilcolinesterase in vitro (AChE), atividades antioxidante e antimicrobiana, e toxicidade em Artemia salina. Os extratos acetónicos foram obtidos por ultrassons (10% m/v), dos quais P. ramosior obteve o rendimento de extração mais elevado (13,49% m/m). Todos os extratos foram testados, no entanto apenas o P. cylindraceus obteve inibição da atividade da AChE (30,2 ± 3,78%). A atividade antimicrobiana foi avaliada usando o método de difusão em poços, contra bactérias Gram-positivas, -negativas e leveduras. P. ramosior revelou não só inibição contra S. aureus e C. albicans (15 e 11 mm, respetivamente), como também a mais elevada atividade antioxidante (método DPPH, 36,4 ± 0,04%). No ensaio em A. salina P. swynnertonnii foi o extrato mais tóxico (LC50 = 0,036 mg/L). Os resultados preliminares obtidos neste trabalho, revelam a potencial biotividade dos extratos de P. cyl...

show abstract

Section: Preparação Dos Extratosunclassified

Section: In Vitro Acetylcholinesterase Inhibitionmentioning

confidence: 99%

Section: Atividade Da Acetilcolinesterase In Vitrounclassified

Section: Determination Of Antioxidant Activity (Dpph)mentioning

confidence: 99%

Section: Determinação Da Atividade Antioxidante (Dpph)unclassified

See 3 more Smart Citations

Biological activity screening of seven Plectranthus species

Ntungwe

Marçalo²,

García³

et al. 2017

BBR

Self Cite

View full text Add to dashboard Cite

show abstract

Vegetable Parts, Herbs, and Essential Oils

Yuliani

Bhandari

Sultana³

2018

Handbook of Vegetables and Vegetable Processing

View full text Add to dashboard Cite

Bioreactor Engineering for Circular Economy: Bioactive Compound Production in Solid‐State Fermentation—Review

Gómez‐Ramos,

Prado‐Barragán,

Rivera

et al. 2024

Chem Eng & Technol

View full text Add to dashboard Cite

Solid‐state fermentation (SSF) has gained considerable attention due to its potential in the production of bioactive compounds from agroindustrial residues, aligning with circular economy targets. This review examines the core bases involved in SSF with a focus on bioreactor engineering. The review underlines the microorganisms metabolic activities under different operational conditions and focuses on engineering challenges encountered in designing packed‐bed bioreactors, including an analysis of the interaction between microbial growth kinetics and transport phenomena. Finally, given its essential role in the scaling‐up process, this review discusses mathematical modeling developed for SSF in packed‐bed bioreactors, establishing a foundation for the future development of more efficient, scalable, and sustainable SSF‐based applications.

show abstract

Optimization of medicinal plant extraction methods and their encapsulation through extrusion technology

Cited by 49 publications

References 15 publications

Biological activity screening of seven Plectranthus species

Biological activity screening of seven Plectranthus species

Vegetable Parts, Herbs, and Essential Oils

Bioreactor Engineering for Circular Economy: Bioactive Compound Production in Solid‐State Fermentation—Review

Contact Info

Product

Resources

About