Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain

Chi, Zhen‐Ming; Zhao, Shuangzhi

doi:10.1016/s0141-0229(03)00119-4

Cited by 147 publications

(92 citation statements)

References 20 publications

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“…The reduction in pullulan production could be attributed to the high viscosity of broth, resulting in a limited availability of nutrients and oxygen to the culture during fermentation. Our results corroborate the findings on pullulan production from A. pullulans [44,50] and R. bacarum [4]. …”

Section: Production Of Pullulan With Time Course As a Functionsupporting

confidence: 92%

“…Agitation also maintains both heat and mass transfer factors during fermentation. Agitation mode of cultivation has been reported best for pullulan production from A. pullulans [51][52][53][54] and R. bacarum [4] in shake-flask fermentations.…”

Section: Pullulan Production With Agitation Mode Of Cultivation As a mentioning

confidence: 99%

“…Structurally, it is a random coil glucan polymer produced mainly by the yeast-like fungus Aureobasidium pullulans. Apart from A. pullulans, other pullulan-producing microorganisms are Tremella mesenterica [1], Cytaria harioti [2], Cyttaria darwinii [3], Rhodotorula bacarum [4], Cryphonectria parasitica [5], Teloschistes flavicans [6], and C. parasitica [7,8]. Commercially, pullulan is produced from A. pullulans.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

2018

J App Biol Biotech

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Pullulan is a random coil glucan polymer produced by Aureobasidium pullulans. It is one of the commercially promising biopolymers frequently used in pharmaceutical, food and cosmetics industries. The aim of present study was to isolate an efficient fungal strain producing melanin-free pullulan. The fungal strains were isolated from fresh and decaying leaves of plants and screened for pullulan production. Fungal isolate PUPY-06 was the best producer of pullulan (2.12%, w/v). Morphological studies revealed the oval and cylindrical shape of its cells, budding and presence of septate as well as pseudohyphae. Colonies of the isolate on agar plate cultures were beige-to-orange colored with smooth, mucoid, and butyrous texture. Morphological and biochemical characterization established the fungal isolate as Rhodosporidium sp. 18s rRNA sequencing, and phylogenetic analysis revealed the isolate to be Rhodosporidium paludigenum. Fourier-transform infrared spectroscopy and nuclear magnetic resonance of crude pullulan confirmed its structural characteristics. Maximum production of pullulan was achieved after 7 days of cultivation in shake-flask fermentations. Agitation mode of cultivation supported the higher production of pullulan in comparison to the stationary mode. R. paludigenum PUPY-06 was found as a novel pullulan-producing strain. This is a first report on pullulan production by R. paludigenum PUPY-06.

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Section: Production Of Pullulan With Time Course As a Functionsupporting

confidence: 92%

Section: Pullulan Production With Agitation Mode Of Cultivation As a mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

2018

J App Biol Biotech

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“…Besides other parameters in fermentation study stirred and non-stirred conditions are also important parameters which affect pullulan production during fermentation by A. pullulans (4,9,14).…”

Section: Pullulans (5)mentioning

confidence: 99%

“…(3,4,7,8,11,14,17). However, till to date no work has been reported for higher production of pullulan above 35 o C.…”

Section: Introductionmentioning

confidence: 99%

Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process

Singh¹,

Gaur²,

Tiwari³

et al. 2012

Braz. J. Microbiol.

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Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42 o C, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.

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Pullulan, Microbial Production Methods

Yang

Thibault

LeDuy

2010

Encyclopedia of Industrial Biotechnology

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Pullulan is a water‐soluble polysaccharide produced through fermentation with Aureobasidium pullulans . Pullulan has been used as specialty food for decades in Japan. Owing to its relatively high price, recent developments on pullulan are mainly focused on its application in biomedical engineering and pharmaceutical industry. Both laboratory and commercial pullulan production methods are reviewed. Among several problems associated with pullulan fermentation, the highly viscous fermentation broth remains the biggest challenge. The viscous non‐Newtonian broth creates major difficulties in mixing, oxygenation, process monitoring, sampling, and downstream product recovery. Many approaches to improve the fermentation efficiency have been attempted. One promising method is to use close clearance impellers, such as reciprocating plate mixer, instead of standard Rushton turbines in order to achieve more uniform mixing. Other unconventional techniques, such as cell immobilization, hyperbaric fermentation, and nonsterile fermentation, have also been discussed. However, these technologies need more research and investigation before they can be implemented at an industrial scale.

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Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain

Cited by 147 publications

References 20 publications

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process

Pullulan, Microbial Production Methods

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