Optimization of Medium Composition and Cultivation Parameters for Fructosyltransferase Production by Penicillium aurantiogriseum AUMC 5605

Abstract

Fructooligosaccharides have been mainly produced by microbial fructosyltransferases (FTase) enzymes. The present work focuses on the optimization of medium composition and cultivation parameters affecting FTase produced by Penicillium aurantiogriseum AUMC 5605 in shake flask cultivation. FTase production was optimized in two steps using DeMeo's fractional factorial design. A 1.46-fold increase in FTase production (105.4 U/mL) was achieved using the optimized culture medium consisting of (g/L): sucrose, 600; yeast extract, 10; K 2 HPO 4 , 5; MgSO 4 ·7H 2 O, 0.5; (NH 4 ) 2 SO 4 , 1.0 and KCl, 0.5. The obtained results showed that the maximum FTase enzyme activity was produced at initial cultivation pH values ranging from 6.0-6.5, at agitation speed of 200 rpm and using vegetative fungal cells as inoculum. Moreover, results showed that optimization of medium composition and some cultivation parameters resulted in an increase of about 93.7% in the enzyme activity than the nonoptimized cultivation conditions after 96 h of cultivation. Additionally, maximum production and specific production rates recorded 2340 U/L/h and 102 U/L/h/g cells, respectively.

SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION

“…Efforts are being made to explore the nature of these prebiotics and to identify microorganisms that produce FFase with a desired stability and suitable activity for industrial use Nascimento et al (2016) utilized Penicillium citreonigrum URM 4459 and applied response surface methodology in shake flasks to determine the fermentation conditions (25.5 ºC, 67.8 h and pH 6.5) that maximize the FTase production obtained, detecting the maximal yield of 301.84 U mL -1 . Farid et al (2015) evaluated the influence of medium composition and cultivation parameters on FTase production by Penicillium aurantiogriseum AUMC 5605 in shake flasks. The maximum FTase enzyme activity was produced at initial cultivation pH values ranging from 6.0-6.5, at an agitation speed of 200 rpm and using vegetative fungal cells as inoculum in the presence of the optimized media.…”

Fructooligosaccharides synthesized by fructosyltransferase from an indigenous coprophilous Aspergillus niger strain XOBP48 exhibits antioxidant activity

“…Further, 10 ml of pre-culture medium was inoculated in fresh 100 ml of the medium in a 250 ml flask and incubated at 28 • C for 72 h at 200 rpm. Uninoculated medium served as control and was incubated under the same condition as the culture (Farid et al, 2015;Park et al, 2001;Sánchez et al, 2010). For the preparation of extracellular enzyme extracts, the fungal mycelia were harvested by filtration using a mutton cloth after 72 h of cultivation.…”