Optimization of medium composition for the production of clavulanic acid by Streptomyces clavuligerus

Wang, Yonghua; Yang, Bo; Ren, Jie; Dong, Meiling; Liang, Dong; Xu, Anlong

doi:10.1016/j.procbio.2004.04.010

Cited by 37 publications

(37 citation statements)

References 13 publications

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“…There is a growing body of literature related to the comparison between airlift and conventional bioreactors for different applications [12][13][14][15][16][17]. Regarding clavulanic acid production, various studies can be found in the literature related to the improvements in the composition of culture media [6,8,[18][19][20], bioreactor operation mode [3,5,7,[21][22][23] and the influence of oxygen supply and agitation condition on CA production by S. clavuligerus in conventional bench scale bioreactor [4]. However, to the best of our knowledge no articles which compare the performances of conventional and non-conventional pneumatic bioreactors on CA production can be found.…”

Section: Introductionmentioning

confidence: 99%

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Cerri

Badino

2012

Bioprocess Biosyst Eng

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In biochemical processes involving filamentous microorganisms, the high shear rate may damage suspended cells leading to viability loss and cell disruption. In this work, the influence of the shear conditions in clavulanic acid (CA) production by Streptomyces clavuligerus was evaluated in a 4-dm(3) conventional stirred tank (STB) and in 6-dm(3) concentric-tube airlift (ALB) bioreactors. Batch cultivations were performed in a STB at 600 and 800 rpm and 0.5 vvm (cultivations B1 and B2) and in ALB at 3.0 and 4.1 vvm (cultivations A1 and A2) to define two initial oxygen transfer conditions in both bioreactors. The average shear rate ([Formula: see text]) of the cultivations was estimated using correlations of recent literature based on experimental data of rheological properties of the broth (consistency index, K, and flow index, n) and operating conditions, impeller speed (N) for STB and superficial gas velocity in the riser (UGR) for ALB. In the same oxygen transfer condition, the [Formula: see text] values for ALB were higher than those obtained in STB. The maximum [Formula: see text] presented a strong correlation with a maximum consistency index (K (max)) of the broth. Close values of maximum CA production were obtained in cultivations A1 and A2 (454 and 442 mg L(-1)) with similar maximum [Formula: see text] values of 4,247 and 4,225 s(-1). In cultivations B1 and B2, the maximum CA production of 269 and 402 mg L(-1) were reached with a maximum [Formula: see text] of 904 and 1,786 s(-1). The results show that high values of average shear rate increase the CA production regardless of the oxygen transfer condition and bioreactor model.

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Section: Introductionmentioning

confidence: 99%

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Cerri

Badino

2012

Bioprocess Biosyst Eng

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“…These authors also observed that, in the absence of glycerol, feeding only ornithine or arginine could not enhance the CA production. Wang et al (2005) obtained an increase of 50% in CA production in shake flasks batch cultivation under optimal concentrations of glycerol (15.0 g.L ), compared to the nonoptimized medium.…”

Section: Introductionmentioning

confidence: 88%

“…04, pp. 499 -506, October -December, 2010 2003; Wang et al, 2005;Teodoro et al, 2006;Ortiz et al, 2007). Recently, Saudagar and Singhal (2007) reported a high CA production (about 1.8 g.L -1 ) in shake flasks utilizing S. clavuligerus MTCC 1142 cultivated in synthetic culture and feeding Lthreonine.…”

Section: −1mentioning

confidence: 99%

“…The amoxicillin/clavulanate combination represents a broad-spectrum antibacterial agent which has been widely used for over 20 years (Buynak, 2006). CA is traditionally produced by Streptomyces clavuligerus using a complex culture media containing soybean derivatives and glycerol as nitrogen, carbon, and energy sources, respectively (Mayer and Deckwer, 1996;Baptista-Neto et al, 2000;Chen et al, 2002;Chen et al, 2003;BaptistaNeto et al, 2005;Neto et al, 2005;Wang et al, 2005;Teodoro et al, 2006;Ortiz et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus

Teodoro

Neto

Araujo

et al. 2010

Braz. J. Chem. Eng.

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-The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp max ) ranged randomly from 430 to 560 mg.L -1 , with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp max ) of 13.7 mg.L -1 .h -1 was obtained with 0.66 g.L -1 of ornithine, 44.2% higher than the Pp max in the control run. In fed-batch experiments, Cp max varied within the narrow range from 1.254 to 1.405 g.L -1 , 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp max , although it influenced only slightly the Cp max . Concerning glycerol, the highest CA production of 1.6 g.L -1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L −1 ) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6:1), on CA biosynthesis.

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“…The production of antimicrobial compound in Streptomyces spp. is greatly influenced by various fermentation parameters such as available nutrients (10), pH, temperature (11), mineral salts (12), precursors (13), and inducers (14). These parameters can be varied from organism to organism (15).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of a New Cold and Alkaline Tolerant Antibiotic Producing Streptomyces sp. From Soil

Rafati

Shahnavaz

Makhdoumi

2016

Jundishapur J Microbiol

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Optimization of medium composition for the production of clavulanic acid by Streptomyces clavuligerus

Cited by 37 publications

References 13 publications

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus

Isolation and Identification of a New Cold and Alkaline Tolerant Antibiotic Producing Streptomyces sp. From Soil

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