Optimization of microbial transglutaminase production using experimental designs

Junqua, Magali; Duran, Robert; Gancet, C.; Goulas, Philippe

doi:10.1007/s002530051124

Cited by 33 publications

(14 citation statements)

References 8 publications

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“…4). Compared to shake flask experiments, this culture condition resulted in an improved TGase volumetric production of approximately 61%, the cell concentration increased four times and the cultivation time for the maximal TGase activity was reduced from 192 to only 50 h. This TGase activity is around two times higher than the obtained by Junqua et al [15], who showed that Streptoverticillium cinnamoneum had a peak of TGase production in approximately 175 h of cultivation at 140 rpm, in a culture medium also containing glycerol as carbon source. Yan et al [21] reported TGase production by Streptoverticillium mobaraense in bioreactor at different agitation speeds (250, 350, and 450 rpm) and fixed aeration rate of 1 vvm.…”

Section: Resultscontrasting

confidence: 53%

“…The economics of TGase production may be enhanced by the optimization of the culture conditions in large-scale processes and the cultivation is affected by medium composition, pH, temperature, aeration, and agitation [14][15][16][17][18][19][20][21]. Currently, TGase production and its improvement have been conducted only in submerged cultures of Streptoverticillium sp.…”

Section: Introductionmentioning

confidence: 99%

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Effects of oxygen volumetric mass transfer coefficient on transglutaminase production by Bacillus circulans BL32

Souza

Rodrigues

Ayub

2009

Biotechnol Bioproc E

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The effects of oxygenation in cultures of _~Åáääìë=ÅáêÅìä~åë BL32 on transglutaminase (TGase) production and cell sporulation were studied by varying the agitation speed and the volume of aeration. Kinetics of cultivations has been studied in batch systems using a 2 L bioreactor, and the efficiency of agitation and aeration was evaluated through the oxygen volumetric mass transfer coefficient (k L a). It was adopted a two-stage aeration rate control strategy: first stage to induce biomass formation, followed by a second stage, in which cell sporulation was stimulated. A correlation of TGase production, spores formation, and oxygen concentration was established. Under the best conditions (500 rpm; 2 vvm air flow, followed by no air supply during stationary phase; k L a of 33.7 h -1 ), TGase production reached a volumetric production of 589 U/L after 50 h of cultivation and the enzyme yield was 906 U/g cells. These values are 61% higher than that obtained in shaker cultures and TGase productivity increased 82%, when k L a varied from 4.4 to 33.7 h -1 . The maximal cell concentration increased four times in relation to shaker cultures and the cultivation time for the highest TGase activity was reduced from 192 h to just 50 h. These results show the importance of bioprocess design for the production of microbial TGase, especially concerning the oxygen supply of cultures and the induction of cell sporulation. © KSBB hÉóïçêÇëW=íê~åëÖäìí~ãáå~ëÉI=ëéçêÉë=Ñçêã~íáçåI=Bacillus circulans=_iPO, ~Éê~íáçåI=~Öáí~íáçåI=îçäìãÉíêáÅ=çñóÖÉå=ã~ëë= íê~åëÑÉê=ÅçÉÑÑáÅáÉåí=Eâ i~F =

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Section: Resultscontrasting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Effects of oxygen volumetric mass transfer coefficient on transglutaminase production by Bacillus circulans BL32

Souza

Rodrigues

Ayub

2009

Biotechnol Bioproc E

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“…Since MTG was excreted into the culture broth, cell disruption was unnecessary for MTG extraction. Zhu et al [8][9][10] and Junqua et al [11] designed a medium by chemical analysis and optimized the fermentation process using a fed-batch culture technique. Later on, a modified down-stream process for purifying MTG was described by Geber et al [12].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of microbial transglutaminase production by Streptoverticillium mobaraense: application of a two-stage agitation speed control strategy

Yan

et al. 2005

Process Biochemistry

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“…Glutamic acid and phosphate concentrations were optimized using a central composite circumscribed (CCC) experimental design for the production of a hybrid antibiotic with Streptomyces lividans TK21 [14]. Transglutaminase production with Streptoverticillium cinnamoneum was optimized with respect to casein and glycerol concentrations in the production medium using CCC experimental design [6]. The effects of pH and temperature on cellulase-free xylanase production of Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Streptomyces peucetius var. caesius N47 cultivation and ?-rhodomycinone production using experimental designs and response surface methods

Kiviharju

Leisola

Eerikäinen

2004

J IND MICROBIOL BIOTECHNOL

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Streptomyces peucetius var. caesius is an aerobic bacterium that produces doxorubicin as a secondary metabolite. A mixture design was applied for the screening of suitable complex medium components in the cultivation of S. peucetius var. caesius N47, which is an epsilon-rhodomycinone-accumulating mutant strain. epsilon-Rhodomycinone is a non-glycosylated precursor of doxorubicin. Best growth results were obtained with soy peptone and beef extract. A central composite face-centered (CCF) experimental design was constructed for the investigation of pH, temperature and dissolved oxygen (DO) effects on the cultivation growth phase. Another CCF was applied to the production phase to investigate the effects of aeration, pH, temperature and stirring rate on epsilon-rhodomycinone production. An increase in cultivation temperature increased both cell growth and glucose consumption rate. Best epsilon-rhodomycinone productivities were obtained in temperatures around 30 degrees C. DO control increased all growth phase responses, but aeration in the production phase coupled with pH decrease resulted in rapid epsilon-rhodomycinone decay in the medium. In non-aerated production phases a pH change resulted in better productivity than in experiments without pH change. A pH increase with a temperature decrease seemed most beneficial for productivity. This implies that dynamic control strategies in batch production of epsilon-rhodomycinone could increase the overall process productivity.

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Optimization of microbial transglutaminase production using experimental designs

Cited by 33 publications

References 8 publications

Effects of oxygen volumetric mass transfer coefficient on transglutaminase production by Bacillus circulans BL32

Effects of oxygen volumetric mass transfer coefficient on transglutaminase production by Bacillus circulans BL32

Enhancement of microbial transglutaminase production by Streptoverticillium mobaraense: application of a two-stage agitation speed control strategy

Optimization of Streptomyces peucetius var. caesius N47 cultivation and ?-rhodomycinone production using experimental designs and response surface methods

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