Optimization of microwave-assisted aqueous extraction of pigments from annatto seeds using Box-Behnken design

Sudha, P.; Manoja, V.; Deepa, J.; Jayakumar, J.; Kishore, S. Ganga; Pandiselvam, R.

doi:10.1007/s13399-023-04046-7

Cited by 6 publications

(2 citation statements)

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“…Annatto is used as seasoning and as coloring to impart yellow, orange-red color to various food products. Various methods are used to improve the yield and stability of annatto ( 7 , 8 ). The seeds are regarded to be the most valuable plant part contributing to promising health benefits.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of bixin on inflammation: a mini review

Shadisvaaran,

Chin,

Mohd-Said

et al. 2023

Front. Nutr.

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Chronic inflammation is the underlying mechanism for many diseases. Thus, inflammatory signaling pathways are valuable targets for new treatment modalities. Natural products have gained interest as a potential source of bioactive compounds which provide health benefits in combating inflammatory-related diseases. Recent reports have linked the medicinal values of Bixa orellana L. with its anti-inflammatory activities. Therefore, this review aims to examine the therapeutic potential of bixin, a major bioactive constituent found in the seeds of B. orellana, on inflammatory-related diseases based on existing in vitro and in vivo evidence. Additionally, the anti-inflammatory mechanism of bixin via signaling pathways is explored and possible toxic effects are addressed. The findings suggest that bixin may ameliorate inflammation via inhibition of toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB), phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) and thioredoxin-interacting protein/NOD-like receptor protein 3 (TXNIP/NLRP3) inflammasome mechanisms. More well-planned clinical studies should be performed to verify its effectiveness and safety profile.

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Section: Introductionmentioning

confidence: 99%

Therapeutic potential of bixin on inflammation: a mini review

Shadisvaaran,

Chin,

Mohd-Said

et al. 2023

Front. Nutr.

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“…These pigments are marketed in the form of powders, liquid extracts and pastes, with their main application being in the food industry. More recent research has shown that green technologies are efficient in reducing the degradation of bioactive compounds by applying modern methods of bixin extraction such as sonication (6.3 5%, w/w) (Yolmeh et al, 2014), microwave-assisted extraction (0.24-0.60%, w/w) (Sudha et al, 2023) and supercritical fluid extraction (0.02-0.10%, w/w) (Albuquerque and Meireles, 2012) which obtain oil and defatted annatto seeds, working as a pretreatment to improve the yield of bixin with mechanical fractionation (2.9-7.6%, w/w) (Alc azar-Alay et al, 2017) and low-pressure solvent extraction (30.0%, w/w) (Zabot et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Technical-economic evaluation of lipo- and water-soluble fractions from annatto seeds by green technology

Franca,

Cavalcanti,

Madruga

et al. 2023

BFJ

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PurposeThe objective of this study was to evaluate the technical-economic process efficiency of obtaining simultaneous lipo-soluble (LSF) and water-soluble (WSF) fractions from annatto seeds.Design/methodology/approachThe batches of annatto seeds were submitted to the refrigerated solid-liquid extraction process in four stages: pre-extraction, aqueous extraction, separation by decantation and filtration. After that, LSF and WSF from annatto seeds were obtained. The process efficiency and the quality of LSF and WSF were analyzed in terms of average yield and bioactive compounds (bixin, norbixin, phenolics and flavonoids) and their antioxidant and antimicrobial activities. Furthermore, they were economically evaluated in terms of costs of manufacturing and profitability parameters.FindingsThe process was efficient in terms of overall average yield (LSF = 8.68% and WSF = 2.76%) (w/w) and in terms of quality, mainly with higher average yields of bixin (82.34% in LSF) and norbixin (29.59% in WSF) (w/w). The concentration of bioactive compounds in the fractions promoted an increase in inhibiting free radicals (DPPH* and ABTS*+) and in the ferric-reducing power (FRAP). LSF showed a minimum inhibitory concentration of 0.06 mg mL-1 for S. aureus and 0.13 mg mL-1 for S. Typhimurium and S. Enteritidis. The lowest manufacturing costs were obtained for the LSF due to its higher extraction yield compared to the WSF. Plants on an industrial scale of 100 and 1000 L were considered economically viable, with a return on investment of 5 and 2 years.Originality/valueThus, fractions (WSF and LSF) can be applied as natural additives, as sources of bioactive compounds for nutraceutical and/or pharmaceutical, and in the development of other innovative processes. These results have practical applicability for pharmaceutical and food industry.Highlights Green processing of annatto seeds obtains fractions rich in antioxidant compounds.Efficiently presents a high yield of bixin and other bioactive compounds.Effective in concentrating compounds that inhibit microbial growth.Fractions are more accessible sources of bioactive compounds for isolation.Cost of manufacturing (COM) and profitability are studied.

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