Optimization of Microwave-Assisted Extraction of Puerarin from<i>Radix Puerariae</i>using Response Surface Methodology

Wu, Zhijun; Hong-sheng, Ruan; Wang, Yanhong; Chen, Zhibao; Cui, Yudong

doi:10.1080/01496395.2012.752749

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…In addition, in order to enable the active ingredients to be fully dissolved in the solvent, the plant material and the organic solvent are usually placed in a heated reflux device to react for a certain period of time, with a reflux temperature of 60-85 • C in general, and extracted 2-3 times [43]. Wu et al [44] have successfully extracted puerarin from kudzu root by the ethanol hot reflux method and studied the effects of different ex-traction factors such as ethanol concentration, extraction temperature, solid-liquid ratio, and extraction times on the extraction rate of puerarin, as well as optimized the extraction conditions using orthogonal tests. The optimum extraction process was obtained as follows: the extraction temperature was 90 • C, the material-liquid ratio was 1:6, the ethanol concentration was 90%, and the extraction time was 2 h. The extraction rate of puerarin was 37.51 ± 0.64 mg/g under this condition, and the experimental results can provide the basis for the large-scale production of puerarin.…”

Section: Solvent Extraction Methodsmentioning

confidence: 99%

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Gao,

Wang,

Huang

et al. 2024

Separations

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Kudzu root (Puerariae lobatae Radix) is the tuberous root of Pueraria lobata, family Leguminosae. Kudzu root contains a variety of beneficial active ingredients such as puerarin, daidzin, daidzein, genistenin, 3′-hydroxy puerarin, β-sitosterol, stigmasterol, arachidic acid, and so on. Modern medical research shows that active ingredients in kudzu root are widely used clinically as raw materials for the treatment of hyperglycemia, non-alcoholic fatty liver disease, myocardial infarction, alcohol addiction, oxidative stress, inflammatory response, and retinal blockage due to their various pharmacological effects such as improving cardiovascular circulation, lowering blood lipids, lowering blood pressure, lowering blood sugar, being antipyretic, being estrogen-like, and relieving alcohol. China has rich resources of kudzu root, and active ingredients are usually extracted before it is made into a preparation, so whether the extraction and separation process is reasonable will directly affect the ease of preparation and the efficacy of the treatment. This paper reviews the process methods for the extraction and separation of active ingredients in kudzu root and its common pharmacological activities. The aim is to provide some references for readers to compare the advantages and disadvantages of various extraction and separation methods as well as understand the active ingredients and pharmacological activities of kudzu root.

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Section: Solvent Extraction Methodsmentioning

confidence: 99%

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Gao,

Wang,

Huang

et al. 2024

Separations

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“…Microwave energy can enhance the penetration of the solvent into the matrix, expediting the release of bioactive compounds, which could significantly increase the extraction rate of the active ingredients from plants. The main advantages of MAE over the conventional extraction techniques are a short extraction time, smaller solvent consumption, and good selectivity [13,14,15].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Solasodine Extracted from Fruits of Solanum nigrum L. by Microwave-Assisted Aqueous Two-Phase Extraction and Analysis by High-Performance Liquid Chromatography

Yang

Wei

et al. 2019

Molecules

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Background: Solasodine is a major bioactive ingredient in Solanum nigrum L. that has strong pharmacological characteristics. Therefore, the development of a simple and effective extraction method for obtaining solasodine is highly important. This study aims to provide a rapid and effective method for extracting solasodine from Solanum nigrum L. by microwave-assisted aqueous two-phase extraction (MAATPE). Methods: First, the high-performance liquid chromatography (HPLC) conditions were established for the detection of solasodine. Then, the aqueous two-phase system (ATPS) compositions were examined. On the basis of the results of single-factor experiments, for a better yield, response surface methodology (RSM) was used to optimize influential factors including the extraction temperature, extraction time and liquid-to-solid ratio. Results: The maximum extraction yield of 7.11 ± 0.08 mg/g was obtained at 44 °C, an extraction time of 15 min, and a liquid-to-solid ratio of 42:1 mL/g in the ATPS consisting of EtOH solvent, (NH4)2SO4, and water (28:16:56, w/w/w). The extraction yield of the alkaloid obtained using this method was markedly higher than those of microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE). Conclusions: In this work, solasodine was extracted by MAATPE for the first time and a high yield was obtained. MAATPE is a simple, rapid, and green technique for extraction from medical plants. Thus, the present study will enable the development of a feasible extraction method of active alkaloids from Solanum nigrum L.

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“…[ 8 ] Later, MAE has been widely used in food, natural products, and traditional Chinese medicine extraction process. [ 8 9 10 11 ]…”

Section: Introductionmentioning

confidence: 99%

Optimization of microwave-assisted extraction of silymarin from Silybum marianum straws by response surface methodology and quantification by high-performance liquid chromatograph method

2018

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Background:Silybum marianum, a member of the Aster family, is a well-known Chinese herb and the source of a popular antioxidant that is extensively used in Asia. The abundant S. marianum straws are still underutilized and wastefully discarded to pollute the environment.Objective:To solve the above problem and better utilize S. marianum straws, the objective of this study was to optimize the conditions for extraction of silymarin from S. marianum straws.Materials and Methods:A combination of microwave-assisted extraction and response surface methodology (RSM) was used for silymarin from S. marianum straws and yield assessment by high-performance liquid chromatography method. The RSM was based on a five-level, four-variable central composite design (CCD).Results:The results indicated that the optimal conditions to obtain highest yields of silymarin were microwave power of 146 W, extraction time of 117 s, liquid-to-solid ratio of 16:1 mL/g, and ethanol concentration of 43% (v/v). Validation tests indicated that under the optimized conditions, the actual yield of silymarin was 6.83 ± 0.57 mg/g with relative standard deviation of 0.92% (n = 5), which was in good agreement with the predicted yield.Conclusions:The exploitation of the natural plant resources present very important impact for the economic development. The knowledge obtained from this work should be useful to further exploit and apply this material.SUMMARY Silymarin has been isolated from Silybum marianum straws by microwave-assisted extraction and response surface methodologyThe results obtained are helpful for the full utilization of S. marianum strawsThe microwave-assisted extraction is a very useful method for the extraction of important phytochemicals from plant materials. Abbreviations used: MAE: Microwave-assisted extraction, RSM: Response surface methodology, HPLC: High-performance liquid chromatography, CCD: Central composite design, ANOVA: Analysis of variance.

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Optimization of Microwave-Assisted Extraction of Puerarin fromRadix Puerariaeusing Response Surface Methodology

Cited by 6 publications

References 28 publications

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Puerariae lobatae Radix: Progress in Extraction, Separation Methods and Pharmacological Activities Research

Enhancement of Solasodine Extracted from Fruits of Solanum nigrum L. by Microwave-Assisted Aqueous Two-Phase Extraction and Analysis by High-Performance Liquid Chromatography

Optimization of microwave-assisted extraction of silymarin from Silybum marianum straws by response surface methodology and quantification by high-performance liquid chromatograph method

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