Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design

Cano, Cristiane Bonaldi; Felsner, Maria Lurdes; Bruns, Roy E.; Matos, Jivaldo do Rosário; Almeida-Muradian, Lígia Bicudo de

doi:10.1590/s0103-50532006000300024

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…In our method, mono-, di-, tri-, and tetrasaccharides are discriminated using mass spectrometry, reducing the number of peaks in each ion chromatogram. The chromatographic separation coupled to mass spectrometry only requires one to separate isobaric species, providing a better peak resolution in comparison to other detectors where all saccharides are determined in one single chromatogram [6,11,24,25,30,35,36].…”

Section: Main Advantages Of Proposed Methodsmentioning

confidence: 99%

“…Several analytical techniques have been employed to determine carbohydrates in honey samples. Indeed, these compounds are mainly determined by high-performance liquid chromatography (HPLC) coupled to different detectors [6,11,25,26]. For example, Arias et al [6] developed an analytical method based on HPLC with UV detection to determine some oligosaccharides.…”

Section: Introductionmentioning

confidence: 99%

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Carbohydrate determination in honey samples by ion chromatography–mass spectrometry (HPAEC-MS)

et al. 2020

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Honey is a complex mixture of carbohydrates, in which the monosaccharides glucose and fructose are the most abundant compounds. Currently, more than 20 oligosaccharides have been identified in different varieties of honey normally at quite low concentration. A method was developed and validated using high-performance anion-exchange chromatography coupled to a mass spectrometry detector to investigate the composition of carbohydrates in honey samples. The method was tested for linearity range, trueness, instrumental and method detection and quantification limits, repeatability, and reproducibility. It was applied to determine seven monosaccharides, eight disaccharides, four trisaccharides, and one tetrasaccharide in various honey samples. The present work describes the composition of sugars in unifloral, multifloral, and some honeydew honey, which were produced and collected by beekeepers in the Trentino Alto-Adige region. Statistical techniques have been used to establish a relationship based on levels of carbohydrates among different Italian honey. The results emphasize that mono-and oligosaccharide profiles can be useful to discriminate different honeys according to their floral characteristics and inter-annual variability. Keywords High-performance anion-exchange chromatography-mass spectrometry (HPAEC-MS). Italian honey. Mono-and oligosaccharide profiles

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Section: Main Advantages Of Proposed Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carbohydrate determination in honey samples by ion chromatography–mass spectrometry (HPAEC-MS)

et al. 2020

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“…The fructose is between 25 and 45%, glucose from 25 to 37%, maltose in the range of 2 and 12%, and sucrose from 0.5 to 3% (Ioannidou et al,2005;Cano et al, 2006). The water content is between 15 and 18% (Salazar et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Honey: Determination of Metal and Sugar Content

Bektashi

Abazi

Popovska

et al. 2021

JAFES

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In this study, five samples of honey (4 from various beekeepers and 1 from a local supermarket) were analyzed to determine the presence of Pb, Fe, Mn, Al, Cu, Cr, Zn, Cd, As, Ni and Se content using atomic absorption spectrometry. Samples were collected from the following locations: 2 from Skopje, 2 from Kumanovo and 1 from Preshevo, Srbia. HPLC was applied to determine sugars in honey samples. Acid and moisture were also determined. The average values of concentration were from 0.033 to 0.055 mg/kg, 0.610to 2.498 mg/kg, 0.052 to 0.377 mg/kg, 2.133 to 3.474 mg/kg, 0.060 to 1.034 mg/kg, 0.005to 0.019 mg/kg, and 0.067 to 0.085 mg/kg for Pb, Fe, Mn, Al, Cu, Cr, and Zn, respectively. Cadmium was determined in one sample (0.040 mg/kg), while the presence of arsenic, nickel and selenium were not observed. The total amount of reductive sugars was within the standard value of min 60 g/100 g honey. The moisture was from 16.6 to 17.6% (max 20%). The acid content was recorded from 9 to 28mEq/1 kg honey (max 50mEq/1 kg honey). The results were compared with the safety standards established from the Ministry of Agriculture, Forestry and Water Economy. In general, the analyzed parameters are within the standards and honey can serve as good dietary source.

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“…Axial points help to extract information about the inner portion of the response surface. 3 Various analytical methods are available for the identification and quantification of both curcumin and piperine. Curcumin was estimated along with other curcuminoids by HPLC.…”

Section: Introductionmentioning

confidence: 99%

Application of Augmented Simplex Centroid Design for Optimization of HPLC Mobile Phase for Estimation of Curcumin and Piperine

Naik¹,

Desai²,

Ramani³

2021

IJPER

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Background: Optimization of mobile phase for high performance liquid chromatography (HPLC) is an exigent task where one factor at a time (OFAT) approach is not preferable as it is time consuming specially for multifaceted samples. The paper describes Augmented Simplex Centroid Design (ASCD) for optimization of ternary mobile phase system for reversed phase HPLC method. The proposed design was utilized for development of mobile phase composition for simultaneous estimation of curcumin and piperine present in dietary supplement. Materials and Methods: Separation was achieved on Sunniest C 18 column (150 mm x 4.6 mm, 5µ). Elution was carried out using in isocratic mode using mobile phase, 0.1 % OPA: ACN: THF (52.7: 37.3: 10.0, v/v/v) at flow rate of 1 ml/min. Sample injection volume was 20 µl. Detection was carried out at 361 nm and run time was 20 min. Results: The proposed method was linear over concentration range of 1 -6 µg/ml for both curcumin and piperine with the regression coefficient 0.9954 and 0.9575, respectively. The limit of detection of the proposed HPLC method for curcumin and piperine were found to be 0.0621 µg/ml and 0.0633 µg/ml, respectively. The limit of quantification of the proposed HPLC method for curcumin and piperine were found to be 0.1863 µg/ml and 0.1899 µg/ml, respectively. The percentage recovery of both curcumin and piperine were found to be in the range of 96.5366 -115.6833 %. The proposed method was precise and robust. Conclusion: The method was successfully applied for standardization of dietary supplement containing curcumin and piperine.

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Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design

Cited by 13 publications

References 25 publications

Carbohydrate determination in honey samples by ion chromatography–mass spectrometry (HPAEC-MS)

Carbohydrate determination in honey samples by ion chromatography–mass spectrometry (HPAEC-MS)

Characterization of Honey: Determination of Metal and Sugar Content

Application of Augmented Simplex Centroid Design for Optimization of HPLC Mobile Phase for Estimation of Curcumin and Piperine

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