Optimization of molecular hydrogen production by Rhodobacter sphaeroides O.U.001 in the annular photobioreactor using response surface methodology

Basak, Nitai; Jana, Asim Kumar; Das, Debabrata

doi:10.1016/j.ijhydene.2014.05.108

Cited by 38 publications

(13 citation statements)

References 41 publications

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“…a o represents the intercept. a i , a ii and a ij denote linear coefficient, quadratic coefficients and interaction coefficient, respectively (Arulmathi et al, 2015;Basak et al, 2014;Maran et al 2013). The mathematical model generated for four independent variables studied was defined as Eq.…”

Section: Optimization Of Screened Culture Medium Componentsmentioning

confidence: 99%

Characterization, production and optimization of lipopeptide biosurfactant by new strain Bacillus pumilus 2IR isolated from an Iranian oil field

Fooladi

Moazami

Abdeshahian

et al. 2016

Journal of Petroleum Science and Engineering

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Section: Optimization Of Screened Culture Medium Componentsmentioning

confidence: 99%

Characterization, production and optimization of lipopeptide biosurfactant by new strain Bacillus pumilus 2IR isolated from an Iranian oil field

Fooladi

Moazami

Abdeshahian

et al. 2016

Journal of Petroleum Science and Engineering

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“…To facilitate determination of the optimum conditions, a statistical experimental design by response surface methodology (RSM) with central composite design (CCD) was used to overcome the disadvantages of the conventional one-factor-at-a-time method [31,32]. RSM with CCD reduces the load of experimental measurements, saves both time and cost [33,34], as well as minimizes errors in determining the effects of parameters It is also capable of determining interactive effects among the treatment variables [31,35].…”

Section: Introductionmentioning

confidence: 99%

“…RSM with CCD reduces the load of experimental measurements, saves both time and cost [33,34], as well as minimizes errors in determining the effects of parameters It is also capable of determining interactive effects among the treatment variables [31,35].…”

Section: Introductionmentioning

confidence: 99%

Photo-fermentational hydrogen production of Rhodobacter sp. KKU-PS1 isolated from an UASB reactor

Assawamongkholsiri

Reungsang

2015

Electronic Journal of Biotechnology

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Background: In this study, the detection of nifH and nifD by a polymerase chain reaction assay was used to screen the potential photosynthetic bacteria capable of producing hydrogen from five different environmental sources. Efficiency of photo-hydrogen production is highly dependent on the culture conditions. Initial pH, temperature and illumination intensity were optimized for maximal hydrogen production using response surface methodology with central composite design. Results: Rhodobacter sp. KKU-PS1 (GenBank Accession No. KC478552) was isolated from the methane fermentation broth of an UASB reactor. Malic acid was the favored carbon source while Na-glutamate was the best nitrogen source. The optimum conditions for simultaneously maximizing the cumulative hydrogen production (H max ) and hydrogen production rate (R m ) from malic acid were an initial of pH 7.0, a temperature of 25.6°C, and an illumination intensity of 2500 lx. H max and R m levels of 1264 ml H 2 /l and 6.8 ml H 2 /L-h were obtained, respectively. The optimum initial pH and temperature were further used to optimize the illumination intensity for hydrogen production. An illumination intensity of 7500 lx gave the highest values of H max (1339 ml H 2 /l) and R m (12.0 ml H 2 /L-h) with a hydrogen yield and substrate conversion efficiency of 3.88 mol H 2 /mol malate and 64.7%, respectively. Conclusions: KKU-PS1 can produce hydrogen from at least 8 types of organic acids. By optimizing pH and temperature, a maximal hydrogen production by this strain was obtained. Additionally, by optimizing the light intensity, R m was increased by approximately two fold and the lag phase of hydrogen production was shortened.

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“…For example, the temperature range of hydrogen production by Rhodobacter sp. was between 31-36°C [2] and 27.5-32.5°C for Rp. palustris CQK 01 [3].…”

Section: Methodsmentioning

confidence: 92%

“…The initial pH and temperature ranges were chosen following a search in the literature. Previous reports indicated that an initial pH of 7 was optimal for cell growth and hydrogen production while hydrogen can not be produced at an initial pH of 5 [2]. Therefore, the initial pH tested in this study was in the range of 6.5-7.5.…”

Section: Methodsmentioning

confidence: 99%

Hydrogen Production by Photo Fermentation via Rhodobacter sp.

Menia

Nouicer

Tebibel

2020

Springer Proceedings in Energy

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Efficiency of photo-hydrogen production is highly dependent on the culture conditions. Initial pH, temperature were optimized for maximal hydrogen production using response surface methodology with central composite design. Photo fermentative hydrogen production is influenced by several parameters, including pH and temperature. Rhodobacter sp. KKU-PS1 was isolated from the methane fermentation broth of an UASB reactor. This study presents the experimental results obtained from Rhodobacter sp. KKU-PS1 cultures as a function of temperature and pH. For this purpose, a complete factorial design was used, for the temperatures of 26, 30 and 34°C and the pH of 6.5; 7 and 7.5. Rhodobacter sp. KKU-PS1 has been isolated from the fermentation of methane. Optimum conditions for maximizing cumulative hydrogen production (H max) were an initial value of pH 7.0 and a temperature of 25°C. The regression models revealed a maximum hydrogen production of 1623 ml at these conditions. KKU-PS1 can produce hydrogen from organic acids. By optimizing the pH and the temperature, a maximal production of hydrogen by this strain was obtained. Validation experiments at calculated optima confirmed these results.

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Optimization of molecular hydrogen production by Rhodobacter sphaeroides O.U.001 in the annular photobioreactor using response surface methodology

Cited by 38 publications

References 41 publications

Characterization, production and optimization of lipopeptide biosurfactant by new strain Bacillus pumilus 2IR isolated from an Iranian oil field

Characterization, production and optimization of lipopeptide biosurfactant by new strain Bacillus pumilus 2IR isolated from an Iranian oil field

Photo-fermentational hydrogen production of Rhodobacter sp. KKU-PS1 isolated from an UASB reactor

Hydrogen Production by Photo Fermentation via Rhodobacter sp.

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