Optimization of Oligomer Chitosan/Polyvinylpyrrolidone Coating for Enhancing Antibacterial, Hemostatic Effects and Biocompatibility of Nanofibrous Wound Dressing

Abstract

A synergistic multilayer membrane design is necessary to satisfy a multitude of requirements of an ideal wound dressing. In this study, trilayer dressings with asymmetric wettability, composed of electrospun polycaprolactone (PCL) base membranes coated with oligomer chitosan (COS) in various concentrations of polyvinylpyrrolidone (PVP), are fabricated for wound dressing application. The membranes are expected to synergize the hygroscopic, antibacterial, hemostatic, and biocompatible properties of PCL and COS. The wound dressing was coated by spraying the solution of 3% COS and 6% PVP on the PCL base membrane (PVP6–3) three times, which shows good interaction with biological subjects, including bacterial strains and blood components. PVP6–3 samples confirm the diameter of inhibition zones of 20.0 ± 2.5 and 17.9 ± 2.5 mm against Pseudomonas aeruginosa and Staphylococcus aureus, respectively. The membrane induces hemostasis with a blood clotting index of 74% after 5 min of contact. In the mice model, wounds treated with PVP6–3 closed 95% of the area after 10 days. Histological study determines the progression of skin regeneration with the construction of granulation tissue, new vascular systems, and hair follicles. Furthermore, the newly-growth skin shares structural resemblances to that of native tissue. This study suggests a simple approach to a multi-purpose wound dressing for clinical treatment.

Electrospun nanofibers synthesized from polymers incorporated with bioactive compounds for wound healing

“…The study also emphasized the potential of natural polymers like chitosan in promoting wound healing [ 241 ]. The study investigated the wound healing effects of ENS Mh (manuka honey) 15%@PVP mats compared to a traditional wound dressing on mice with circular wounds on their dorsal back [ 242 ]. The advanced dressing with ENS Mh@PVP showed great results in wound healing.…”

Chitosan-Based Biomaterials for Tissue Regeneration

“…Doan and colleagues reported that 95% of the wound area healed after 10 days post-treatment with the multilayer membrane [ 111 ]. The newly grown skin shared a resemblance with the native tissue and illustrated the progressive construction of vascular systems, hair follicles, etc.…”

“…Chitosan-based membranes and scaffolds are known for their antibacterial properties. A multilayer membrane for facilitating wound healing, which consists of oligomer chitosan, polycaprolactone, and polyvinylpyrrolidone, was assembled by Doan and colleagues [ 111 ]. According to the results, the composite membrane was able to inhibit the growth of several bacteria types.…”

Biodegradable nanocomposites prepared from poly(butylene adipate terephthalate) and silver nanoparticles for applications as disposable medical equipments

“…As a non-ionic polymer, PVP exhibits unique physical and chemical features such as low toxicity, solubility in both water and organic solvents, acid and alkali resistance, oxidation resistance, and stabilizing effect on colloids. [14][15][16] PVP can be used as a colloidal stabilizer, surfactant, dispersant, and even reducing agent. 17 Wang et al used PVP as dispersant in the preparation of AgNPs.…”