Optimization of Paramecium tetraurelia growth kinetics and its sensitivity to combined effects of azoxystrobin and cyproconazole

Abstract

For using an organism for ecotoxicological studies, it is important to understand its model of growth under some physicochemical parameters that influence with a direct manner its development. The knowledge of the optimal parameters for the paramecia development leads to realize a model of growth with clear stages. For that, we tried to study the influence of temperature, pH, nutrient density in the culture medium and pollution on the growth of Paramecium tetraurelia. Obtained results showed that paramecia prefer definite conditions. However, the growth was better in temperatures included between 25 and 30°C, with preference to increased temperatures and a slightly acidic pH. The culture medium was prepared from the mixture of several vegetables which the rates were studied depending to the growth. The combined effects of azoxystrobin and cyproconazole on the growth kinetics were studied. Results showed that treatment affected the population growth, the generation time and the velocity of generation.

Physiological and biochemical changes observed in alternative cellar model: Paramecuim tetraurelia treated with paracetamol

“…We also observed changes in our forms paramecia treated these defects result in budding at the plasma membrane and cytoplasm with the appearance of large bright spots causing a change in the morphology of the paramecium. These results are in agreement with those of (Azzouz et al, 2011) and Venkateswara Rao et al (2006, 2007. (Amanchi and Hussain, 2010) Our results confirm those obtained with paramecia exposed to fenthion and underwent a series of behavioral and morphological changes such as alteration of shape and budding intensive ultimately lead to the total destruction of the cells in a dosedependent (Friedl, 2003;Mills, 1998).…”

“…The cells were subcultured every three days to maintain a good culture and experiments are performed in logarithmic growth phase. (Azzouz et al, 2011). N-(4-hydroxyphenyl)…”

Antioxidant activities and lipid peroxidation in the freshwater bioindicator Paramecium sp. exposed to hydrazine carboxylate (Bifenazate)

“…To minimize this damage, organisms developed antioxidant defence mechanism, whereas the failure to detoxify excess (ROS) production may lead to inactivation of enzymes, breakdown of both proteins and (DNA), and may also cause peroxidation of lipids (Bandyopadhyay et al, 2007). Several authors used protozoa, especially Paramecia, as an alternative model to demonstrate the harmful effects of certain pesticides on the locomotor behavior, biochemical metabolism and physiology of these organisms (Sbartai et al, 2009(Sbartai et al, , 2012Azzouz et al, 2011Azzouz et al, , 2015Saib et al, 2014;Moumeni et al, 2016). After many years of hard work, Paramecium has been determined to serve as a modern model organism that would make specific contributions to the cell, the molecular and the developmental biology (Van Houten, 2019).…”