Optimization of Pathogenicity Tests for Selection of Native Isolates of Entomopathogenic Fungi Isolated from Citrusgrowing Areas of México on Adults of<i>Diaphorina citri</i>Kuwayama (Hemiptera: Liviidae)

Gandarilla-Pacheco, Fátima Lizeth; Galán-Wong, Luis J.; López-Arroyo, J. Isabel; Rodríguez-Guerra, Raúl; Quintero-Zapata, Isela

doi:10.1653/024.096.0125

Cited by 31 publications

(13 citation statements)

References 31 publications

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“…bassiana , M. anisopliae , Hirsutella citriformis , Acrostalagmus aphidium , Fusarium culmorum , Lecanicillium lecanii , Cladosporium oxysporum , Capnodium citri , and Stemphylium sp. have been reported to control different insect pests, but only a few studies have reported the pathogenicity of C. javanica against D. citri (Faria & Wraight, ; Gandarilla‐Pacheco, Galán‐Wong, López‐Arroyo, Rodríguez‐Guerra, & Quintero‐zapata, ; Medina‐Córdova, Rosales‐Mendoza, Hernández‐Montiel, & Angulo, ; Meyer, Hoy, & Boucias, ; Vega et al, ). Nguyen et al () isolated two highly virulent strains of C. javanica against diamondback moth Plutella xylostella with respective LT 50 values of 2.52 and 2.55 days.…”

Section: Discussionmentioning

confidence: 99%

Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Zhang

Guo

et al. 2018

MicrobiologyOpen

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The Asian citrus psyllid, Diaphorina citri Kuwayama, is the most serious pest of citrus worldwide. It acts as a vector for a group of phloem‐limited bacteria ( Candidatus Liberibacter spp.) that causes Huanglongbing (HLB) disease. Thus, D. citri management is an important strategy against HLB, and biological control is currently considered as the most effective method because of the unsustainable and negative side effects of chemical control. Here, we isolated a new strain of entomopathogenic fungus, Cordyceps javanica (GZQ‐1), from one cadaver of D. citri adult based on its morphological and phylogenetic data. Five conidial concentrations of the C. javanica pathogen (1 × 10 3 , 1 × 10 4 , 1 × 10 5 , 1 × 10 6 , and 1 × 10 7 conidia/ml) were assessed against six life stages of D. citri (1st‐5th instar nymphs and adults). Results showed that C. javanica GZQ‐1 was highly pathogenic to D. citri nymphs (69.49%–90.87% mortality) and adults (69.98% mortality). The LC 50 and LT 50 values of C. javanica against 1st‐2nd instar (younger), 3rd‐4th instar (middle aged), 5th instar (older), and adults were 1.20 × 10 5 , 1.10 × 10 6 , 4.47 × 10 6 , 8.12 × 10 6 conidia/ml and 4.25, 4.51, 5.17, 5.49 days, respectively. Moreover, glasshouse experiments indicated that this C. javanica GZQ‐1 caused higher infection rates of D. citri adults compared to two other fungal strains we previously isolated in the laboratory, Cordyceps fumosorosea (IF010) and Metarhizium anisopliae (CNGD7).

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Section: Discussionmentioning

confidence: 99%

Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Zhang

Guo

et al. 2018

MicrobiologyOpen

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“…Notable examples are Diaprepes citrus root weevil (Diaprepes abbreviates), black citrus aphid (Toxoptera citricida), citrus longhorn beetle (Anoplophora chinensis), glassy-winged sharpshooter (Homalodisca vitripennis), and the Asian citrus psyllid (Diaphorina citri) [3,6,7]. However, aside from foliar spraying of targeted pests or plants with fungal entomopathogens suspension to offer short term protection for plants against pests infestation [7], a practice which reduces the efficacy of entomopathogenic fungi due to the exposure of fungal spores to environmental conditions such as UV radiation, high temperature, low humidity, etc. [8], previous findings have revealed the possibility of establishing these entomopathogenic fungal species as endophytes in plants [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Seedling Age on Colonization Patterns of Citrus limon Plants by Endophytic Beauveria bassiana and Metarhizium anisopliae and Their Influence on Seedlings Growth

Bamisile

Akutse

Dash

et al. 2020

JoF

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The inoculation methods, the fungal strains, and several other factors are known to influent the success of fungal entomopathogens colonization in plants. The physiological status of the plant could also be another determinant. In the present study, the ability of three strains of Beauveria bassiana and one strain of Metarhizium anisopliae to successfully colonize Citrus limon plants and the influence of seedling age on endophytic colonization success was examined. Three, 4, and 6 months old seedlings were inoculated with 10 mL of 1 × 108 conidial·mL−1 suspensions of each of the four fungal strains via foliar spraying. All fungal strains successfully colonized citrus seedlings and were sustained up to 2 months in colonized plants irrespective of the seedling age, with differences in the mean percentage colonization recorded at various post-inoculation periods among the fungal strains. The highest percent endophytic fungi recovery rate was recorded in the 3 months old seedlings, where fungal mycelia of inoculated fungi were successfully re-isolated from 65.6% of the untreated newly developed leaf and stem tissues. One strain of B. bassiana, BB Fafu-12, significantly improved seedling height and leaf number. The study demonstrates the influence of seedling age on B. bassiana and M. anisopliae successful colonization in the citrus plant.

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“…Entomopathogenic fungi are important natural enemies of pests because they infect a large number of arthropods, and under favorable conditions, they can initiate epizootics in the field (Alves 1998). The pathogenicity of certain fungal species to D. citri has been reported, such as Isaria fumosorosea, Beauveria bassiana, Hirsutella citriformis, Lecanicillium lecanii, L. muscarium, L. longisporum, Metarhizium anisopliae and M. brunneum (Ascomycota: Hypocreales) (Avery et al 2009;Casique-Valdes et al 2011;Gandarilla-Pacheco et al 2013;Hall et al 2012;Hoy et al 2010;Hunter et al 2011;Lezama-Gutiérrez et al 2012;Meyer et al 2007Meyer et al , 2008Padulla and Alves 2009;Pinto et al 2012;Stauderman et al 2012;Subandiyah et al 2000;Yang et al 2006). The entomopathogenic fungi I. fumosorosea, B. bassiana, L. lecanii and M. anisopliae have been reported as pathogens of T. citricida (Balfour and Khan 2012;Hernández-Torres et al 2006;Pick et al 2012;Poprawski et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Transmission potential of the entomopathogenic fungi Isaria fumosorosea and Beauveria bassiana from sporulated cadavers of Diaphorina citri and Toxoptera citricida to uninfected D. citri adults

et al. 2016

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Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of the causal agent of Huanglongbing disease, and Toxoptera citricida Kirkaldy (Hemiptera: Aphididae) is the vector of the citrus tristeza virus. D. citri and T. citricida share the same habitat and both are susceptible to Beauveria bassiana and Isaria fumosorosea. Therefore, the aim of this study was to evaluate the horizontal transmission of B. bassiana ESALQ-PL63 and I. fumosorosea ESALQ-1296 between D. citri cadavers and uninfected D. citri adults, as well as between T. citricida cadavers and uninfected D. citri adults under laboratory and semifield conditions. In the laboratory, the presence of one to ten D. citri cadavers infected with B. bassiana and I. fumosorosea in citrus plants resulted in mortality rates of D. citri adults ranging from 51.2 to 81.9 % and 36.2 to 68 %, respectively. When T. citricida cadavers were used, the mortality rates of uninfected D. citri adults ranged from 35.4 to 87.7 % with B. bassiana and from 41.7 to 80.4 % with I. fumosorosea. The horizontal transmission was also confirmed under semi-field conditions. The results indicate that the control of D. citri using I. fumosorosea and B. bassiana can be promoted through conidial cycling in T. citricida, by generating new infection cycles, which may contribute to the overall performance of microbial control in citrus. Keywords Biological control Á Citrus pests Á Entomopathogen Á Horizontal transmission Á Diaphorina citri Á Toxoptera citricida

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Optimization of Pathogenicity Tests for Selection of Native Isolates of Entomopathogenic Fungi Isolated from Citrusgrowing Areas of México on Adults ofDiaphorina citriKuwayama (Hemiptera: Liviidae)

Cited by 31 publications

References 31 publications

Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Identification of a new Cordyceps javanica fungus isolate and its toxicity evaluation against Asian citrus psyllid

Effects of Seedling Age on Colonization Patterns of Citrus limon Plants by Endophytic Beauveria bassiana and Metarhizium anisopliae and Their Influence on Seedlings Growth

Transmission potential of the entomopathogenic fungi Isaria fumosorosea and Beauveria bassiana from sporulated cadavers of Diaphorina citri and Toxoptera citricida to uninfected D. citri adults

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