Optimization of PCR-based methods for rapid detection of Campylobacter jejuni, Campylobacter coli and Yersinia enterocolitica serovar 0:3 in wastewater samples

Alexandrino, Maria; Grohmann, Elisabeth; Szewzyk, Ulrich

doi:10.1016/j.watres.2003.10.036

Cited by 39 publications

(18 citation statements)

References 22 publications

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“…It is the most widely used molecular technique in the detection of microorganisms in water samples. Indeed, PCR-based methods have been utilized to detect waterborne pathogens such as Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica, E. coli, and Salmonella [19,34].…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Recommended advanced techniques for waterborne pathogen detection in developing countries

Alhamlan

Al‐Qahtani

Al-Ahdal

2015

J Infect Dev Ctries

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The effect of human activities on water resources has expanded dramatically during the past few decades, leading to the spread of waterborne microbial pathogens. The total global health impact of human infectious diseases associated with pathogenic microorganisms from landbased wastewater pollution was estimated to be approximately three million disability-adjusted life years (DALY), with an estimated economic loss of nearly 12 billion US dollars per year. Although clean water is essential for healthy living, it is not equally granted to all humans. Indeed, people who live in developing countries are challenged every day by an inadequate supply of clean water. Polluted water can lead to health crises that in turn spread waterborne pathogens. Taking measures to assess the water quality can prevent these potential risks. Thus, a pressing need has emerged in developing countries for comprehensive and accurate assessments of water quality. This review presents current and emerging advanced techniques for assessing water quality that can be adopted by authorities in developing countries.

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Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Recommended advanced techniques for waterborne pathogen detection in developing countries

Alhamlan

Al‐Qahtani

Al-Ahdal

2015

J Infect Dev Ctries

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“…In the only previous study of wastewater contamination by campylobacters where Q-PCR was used, C. jejuni/coli DNA was not detected in untreated or treated wastewater in two WWTPs in Germany (Alexandrino et al, 2004). Our study of Swiss wastewater plants is based on more WWTPs, but less replicates per WWTP, than the studies cited above.…”

Section: September 2009mentioning

confidence: 90%

Quantification of Thermotolerant Campylobacter in Swiss Water Treatment Plants, by Real‐Time Quantitative Polymerase Chain Reaction

Rinsoz

Hilfiker²,

Oppliger

2009

Water Environment Research

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“…in water and wastewater were successfully achieved employing various PCR protocols, using diverse primers [18,[85][86][87][88][89][90]. Most of these protocols aimed at the detection of the presence or absence of campylobacters; whereas other protocols [91] used real-time PCR for obtaining quantitative results.…”

Section: Detection In Water and Wastewatermentioning

confidence: 99%

Campylobacter in the environment: A major threat to public health

Abulreesh¹,

Organji²,

Elbanna³

et al. 2017

APJTD

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Optimization of PCR-based methods for rapid detection of Campylobacter jejuni, Campylobacter coli and Yersinia enterocolitica serovar 0:3 in wastewater samples

Cited by 39 publications

References 22 publications

Recommended advanced techniques for waterborne pathogen detection in developing countries

Recommended advanced techniques for waterborne pathogen detection in developing countries

Quantification of Thermotolerant Campylobacter in Swiss Water Treatment Plants, by Real‐Time Quantitative Polymerase Chain Reaction

Campylobacter in the environment: A major threat to public health

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