Optimization of Peroxidase Activity of Turnip (<i>Brassica rapa</i>) Using Response Surface Methodology

Dahdouh, Amel; Bachir‐bey, Mostapha; Katı, Djamel Edine

doi:10.2478/aucft-2020-0017

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…The pH of the medium impacts the microbe's net charge, affecting its enzymatic production physiology (Makapela et al 2016). Turnip POX at pH 8 and 30 °C gave maximum activity (4027 U/mg) (Dahdouh et al 2020). Falade et al (2019) reported 5.9 pH at 30 °C in Ensifer adhaerens POX.…”

Section: Effects Of Ph Value Temperature and Incubation Days On Pox A...mentioning

confidence: 99%

Physicochemical parameters optimization and peroxidase characterization from Aspergillus niger native strain by solid-state fermentation for improved dye decolorization

Ashraf

Afroz

Anwar

2023

BioRes

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The hyper yield of peroxidase (POX) was investigated for a novel native Aspergillus niger strain identified by 18S RNA analysis. A. niger strains sequences were submitted to GenBank; IDs allotted were MN611114.1 (BMB 17) and MN559756.1 (BMB-18). The identified Aspergillus strains in combination showed enhanced (POX) activity (601.5 U/mL) by solid-state fermentation in comparison to their individual activities. POX was purified by ammonium sulfate, and size exclusion gel chromatography exhibited a 7.83-fold increase in POX concentration (13.3 U/mg) in comparison to BMB17 and BMB 18 (11.8 & 7.6 U/mg respectively). The best POX activity was obtained with pH 6.5, 37 °C, and 5 days of incubation. Using guaiacol as substrate, POX showed maximum activity (Vmax) of 537 U/mL with a corresponding Michaelis constant (Km) value of 126 µM. Calcium chloride worked as a POX activator at 300 & 400 mM. Zinc sulfate (500 mM), EDTA (5 mM); ethanol, propanol, and acetonitrile (50%) inhibited (18-30%) POX. Urea (1M), and copper sulfate (500 mM) strongly inhibited POX up to 40%. Polysorbate-80 (1%) slightly reduced the POX by 10% to 15%. BMB17+18-induced promising dye decolorization (88-98%) against all vat dyes, methylene blue, and phenol red.

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Section: Effects Of Ph Value Temperature and Incubation Days On Pox A...mentioning

confidence: 99%

Physicochemical parameters optimization and peroxidase characterization from Aspergillus niger native strain by solid-state fermentation for improved dye decolorization

Ashraf

Afroz

Anwar

2023

BioRes

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“…Peroxidases (PODs) (E.C 1.1.11.7), which are in the oxidoreductase enzyme class, are responsible for electron exchange from a natural substrate (hydrogen peroxide, H 2 O 2 ) to another synthetic compound (guaiacol) 1 . They can contribute to H 2 O 2 reduction while oxidizing a second substrate.…”

Section: Introductionmentioning

confidence: 99%

“…H 2 O 2 ) to another synthetic compound (guaiacol). 1 They can contribute to H 2 O 2 reduction while oxidizing a second substrate. These enzymes are among the key critical antioxidant biocatalysts involved in detoxifying reactive oxygen species (ROS) and preventing oxidative stress.…”

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confidence: 99%

Development of a new affinity chromatography method for purification of horseradish peroxidase enzyme

Gerni,

Özdemir

2023

Biotech and App Biochem

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In this study, benzohydroxamic acid molecules were synthesized from methyl 4‐amino‐2‐methoxy, methyl 4‐amino‐3‐nitro, methyl 4‐amino‐3‐methyl, and methyl 4‐amino‐3‐chloro benzoate molecules, and the horseradish peroxidase (HRP) enzyme was purified in one step using the affinity chromatography technique for the first time. The IC50 and Ki values for the 4‐amino 3‐methyl benzohydroxamic acid molecule were 0.136 and 0.132 ± 0.054 μM, respectively, while the IC50 and Ki values for the 4‐amino‐3‐nitro benzohydroxamic acid molecule were 56.00 and 51.90 ± 9.90 μM, respectively. It was found that the IC50 and Ki values for the 4‐amino‐3‐chloro benzohydroxamic acid molecule were 218.33 and 175.67 ± 43.78 μM, respectively, whereas the IC50 and Ki values for the 4‐amino‐2‐methoxy benzohydroxamic acid molecule were 306.00 and 218.00 ± 68.80 μM, respectively. The HRP enzyme was synthesized from 4‐amino‐2‐methoxy hydroxamic acid column with a 35.97% yield 601.13 times, 4‐amino‐3‐nitro hydroxamic acid column, with a 14.00% yield 404.11 times, 4‐amino‐3‐methyl hydroxamic acid column with an 8.70% yield 394.88 times, and 4‐amino‐3‐chloro hydroxamic acid column with a 4.48% yield 284.85 times. Thus, the HRP enzyme was purified in a single step with hydroxamic acids, and its molecular weight was found to be 44 kDa. The optimum pH was 8.0, the optimum temperature was 15°C, and the optimum ionic strength was 0.4 M for the purified HRP enzyme.

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A new peroxidase from the roots of the Algerian white turnip (Brassica rapa, variety rapa): extraction, purification, characterisation, and antioxidant potential

Dahdouh

Boucherba

Bouacem

et al. 2021

Biocatalysis and Biotransformation

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Optimization of Peroxidase Activity of Turnip (Brassica rapa) Using Response Surface Methodology

Cited by 3 publications

References 30 publications

Physicochemical parameters optimization and peroxidase characterization from Aspergillus niger native strain by solid-state fermentation for improved dye decolorization

Physicochemical parameters optimization and peroxidase characterization from Aspergillus niger native strain by solid-state fermentation for improved dye decolorization

Development of a new affinity chromatography method for purification of horseradish peroxidase enzyme

A new peroxidase from the roots of the Algerian white turnip (Brassica rapa, variety rapa): extraction, purification, characterisation, and antioxidant potential

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