Optimization of process parameters for gelatin extraction from the skin of Blackspotted croaker using response surface methodology

Abstract

Utilization of waste from fish processing industry for production of value added products has attracted substantial attention. Blackspotted croaker (Protonibea diacanthus) is a marine fish having the potential of abundant supply of raw skins for production of gelatin. This study was aimed to optimize the extraction conditions for gelatin production from skin of Blackspotted croaker. Response surface methodology (RSM) was adopted by following central composite design to determine the optimal conditions of four independent variables namely concentration of NaOH (X1), soaking time (X2), extraction temperature (X3) and extraction time (X4) for three response variables namely yield, gel strength and melting point. The models obtained by RSM produced a satisfactory fit to the data with respect to gelatin extraction (for gelatin yield, R (2) = 0.867, P = 0.0003; for gel strength, R (2) = 0.837, P = 0.007; for melting point, R (2) = 0.765, P = 0.01). Based on these models, the optimum conditions to achieve the predicted maximum values were: yield of 17.21 % at X1 = 0.23 %, X2 = 46.19 min, X3 = 55.29 °C and X4 = 17.29 h; gel strength of 422.69 g at X1 = 0.22 %, X2 = 44.56 min, X3 = 59.02 °C and X4 = 15.35 h and melting point of 23.48 °C at X1 = 0.20 %, X2 = 46.68 min, X3 = 56.23 °C and X4 = 15.21 h. It can be concluded from the present study that Blackspotted croaker skin is a prospective source to produce gelatin in good yield with desirable quality attributes comparable to commercially available mammalian gelatins.

Optimisation of gelatine extraction from chicken feet-heads blend using Taguchi design and response surface methodology

“…Based on Figure 2a, extraction yield was positively influenced by higher temperature and time extraction. This observation was the same as that of Jakhar et al (2014) and Kim (2017). The higher temperatures used for gelatine extraction destroyed the hydrogen bonds stabilising the collagen structure, thus contributing to an efficient denaturation of this later, and a higher gelatine yield (Omar and Sarbon, 2016).…”

Characterization of the Complete Mitochondrial Genome Sequences of Three Croakers (Perciformes, Sciaenidae) and Novel Insights into the Phylogenetics

“…There are only 9 studies on N. coibor , P. diacanthus , and A. amoyensis [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ] involving active material extraction, nosography, and mitochondrial phylogenetic and microsatellite variation. There have been some studies concerning mitochondrial genome in the genera Nibea , Protonibea , and Argyrosomu , the family Sciaenidae and order Perciformes.…”

Pemanfaatan Hasil Tangkapan Sampingan Ikan Cucut dan Ikan Pari dalam Pembuatan Gelatin (By-Catch Utilization of Shark and Stingray Fish on Producing of Gelatin)

“…Jiang (2013) melaporkan bahwa hidrolisis optimum sisik ikan pada kondisi suhu 50°C, pH 9, menggunakan enzim alkaline proteinase 3% (b/b) selama 6 jam menghasilkan rendemen gelatin sebesar 48,1% (b/b). Pada kondisi hidrolisis optimum dengan menggunakan NaOH sebagaimana dilakukan Jakhar et al (2012) (Jones 1977;Mahmoodani et al 2012). Kekuatan gel selalu berhubungan dengan karakteristik alami matriks protein bahan baku (Jamilah et al 2011).…”