Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergillus species

Ellaiah, P; Adinarayana, K; Bhavani, Yellankula; Padmaja, P; Srinivasulu, B

doi:10.1016/s0032-9592(02)00188-7

Cited by 172 publications

(135 citation statements)

References 12 publications

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“…Furthermore, the coded values were substituted in the regression equation to obtain the predicted enzyme activity, and a good correlation between the measured and predicted responses was noticed, as seen from their R 2 value (0.8950). The findings of the present investigation compare well with the work of Ellaiah et al, (2002) on response surface optimization of the critical medium components for the production of alkaline protease by a newly isolated Bacillus species. Similarly, CCD techniques have been used to optimize maximum xylanase yields by Schizophyllum commune and Thermomyces sp with activity of 5.74 U/ml and 2.74 U/ml under submerged conditions (Haltrich et al, 1993;Purkarthofer et al, 1993).…”

Section: Resultssupporting

confidence: 80%

Statistical Analysis of Main and Interaction Effects to Optimize Xylanase Production under Submerged Cultivation Conditions

Mullai¹,

Fathima²,

Rene³

2010

JAS

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In recent years, xylanase has become an essential option for environmental friendly industrial biotechnological applications and there is a rising demand for large scale production. In this study, a Bacillus species 2129 was tested for the xylanase production under submerged cultivation conditions. Maximum xylanase activities were achieved using oat as the substrate and by optimizing process conditions such as substrate concentration, pH and nitrogen source using statistically significant design of experiments, employing the response surface methodology (RSM) concept. Under optimized conditions there was an 8% increase in the enzyme activity and results from statistical approximation in the form of analysis of variance (ANOVA) shows that the squared effects of the variables were significant than both the main and interaction effects.

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Section: Resultssupporting

confidence: 80%

Statistical Analysis of Main and Interaction Effects to Optimize Xylanase Production under Submerged Cultivation Conditions

Mullai¹,

Fathima²,

Rene³

2010

JAS

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“…The SSF process has the potential to significantly reduce the enzyme production costs because of lower energy requirements, increased productivity, smaller effluent volumes and simpler fermentation equipment. Agro-industrial residues are generally considered best substrates for the SSF processes and enzyme production by SSF is not an exception to that [4]. SSF uses agro-industrial waste as support and/or carbon source for production of various value added products, such as single cell protein, industrial enzymes, secondary metabolites, and fine chemicals [5].…”

Section: Introductionmentioning

confidence: 99%

Solid State Fermentation of Overheated Soybean Meal (Waste) For Production of Protease Using Aspergillus Oryzae

Thakur¹,

Nemade²,

Sharanappa³

2015

IJIRSET

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Biochemical conversion of agro-industrial wastes is an effective way of value addition. During the extraction of oil from soybean seed, due to supply of excessive heat or being retained for more time in desolventizer, the meal quality is reduced due to overheating and hence rejected for animal feed. The solid state fermentation using Aspergillus oryzae (NCIM No.649) to recover protease shows excellent potential of the overheated soybean meal (waste) as source. During the study the effect of varying particle size, pH, moisture content, incubation time and Protease activity has been measured.

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“…The microbial amylases meet industrial demands with a large number of them available commercially and have almost completely replaced chemical hydrolysis of starch in starch processing industry (Patel et al 2005). Solid state fermentation (SSF) constitutes an interesting alternative to most popular Submerged State Fermentation since the metabolites so produced are concentrated and purification procedures are less costly (Ellaiah et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Cultural Conditions for Solid State Fermentation of Amylase Production by Aspergillus species

Gautam

Karki

Panta

2013

Nepal Journal of Science and Technology

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Amylase is an amylolytic enzyme used in food industry which is generally produced by Aspergillus spp. under solid state fermentation. The present study is concerned with the isolation, screening and selection of suitable strains of Aspergillus spp. and optimization of cultural conditions for the biosynthesis of amylase. Rice and wheat brans were used as substrates which are readily available inexpensive raw materials for amylase production. From 85 samples of rice and wheat grains, 55 colonies obtained on potato dextrose agar (PDA) were suspected to be Aspergillus oryzae and only 35 colonies possessed the morphological characteristics similar to that of A. oryzae indicating the isolates were most likely the strains of A. oryzae. Of all the fungal isolates of Aspergillus spps., Asp.31 gave maximum production of amylase (720.782 IUgds -1 ) in solid state fermentation media. This strain was selected as a parental strain for optimization for cultural conditions. The obtained data were analyzed using SPSS-11.5 program. Of all the substrates (rice bran, wheat bran and their mixture), rice bran was the best for producing amylase of highest activity 611.614 IUgds -1 .The highest enzyme activity of 698.749 IUgds -1 was observed at 50% initial moisture level of the substrate. The optimum temperature was 25ºC for producing the crude amylase enzyme with amylase activity of 577.757 IUgds -1 .

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Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergillus species

Cited by 172 publications

References 12 publications

Statistical Analysis of Main and Interaction Effects to Optimize Xylanase Production under Submerged Cultivation Conditions

Statistical Analysis of Main and Interaction Effects to Optimize Xylanase Production under Submerged Cultivation Conditions

Solid State Fermentation of Overheated Soybean Meal (Waste) For Production of Protease Using Aspergillus Oryzae

Optimization of Cultural Conditions for Solid State Fermentation of Amylase Production by Aspergillus species

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