Optimization of process variables for enhanced production of urease by indigenous Aspergillus niger strains through response surface methodology

Khan, Yasir Mehmood; Munir, Hira; Anwar, Zahid

doi:10.1016/j.bcab.2019.101202

Cited by 18 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3b). In another study, the fungal urease of Aspergillus exhibited maximum production and urease activity at 35 °C and the least activity was at 50 °C (Khan et al 2019). Others reported 35 °C and 40 °C as the optimum temperature for urease activity (Danial et al 2015;Fathima and Jayalakshmi 2012).…”

Section: Purification and Characterization Of Uamentioning

confidence: 91%

“…3c). There was an increase in urease activity with an increase of pH from 3.0-9.0 (Khan et al 2019). Some bacterial ureases exhibited high activity in alkaline conditions (pH of 9.0) (Phang et al 2018), while some had maximum urease activity at pH 8 (Danial et al 2015;Mirbod et al 2002).…”

Section: Purification and Characterization Of Uamentioning

confidence: 94%

“…Two fungal isolates of the genus Aspergillus had an optimum pH of 8.0 and 8.5 (Kappaun et al 2018). In general, the fungal urease had their maximum activity in the basic medium, while bacterial urease tended to be more variable (Khan et al 2019). The higher thermostability favours the application of UA in a variety of industrial and environmental engineering applications.…”

Section: Purification and Characterization Of Uamentioning

confidence: 99%

“…Except for the orthogonal array design-based approach for urease production using A. niger (Bakhtiari et al 2006) and response surface methodology (RSM) (Khan et al 2019), there are no statistical optimization-based reports available for other bacteria. This is the first report on the statistical optimization of urease production of B. halodurans.…”

Section: Statistical Optimization For Urease Productionmentioning

confidence: 99%

See 3 more Smart Citations

Optimization of urease production by Bacillus halodurans PO15: a mangrove bacterium from Poovar mangroves, India

Nathan

Jasna

Parvathi

2020

Mar Life Sci Technol

View full text Add to dashboard Cite

Mangrove ecosystems are one of the most versatile habitats for microorganisms with a high potential for producing a variety of extracellular hydrolytic enzymes. In this study, bacteria with urease activity, enzymes that catalyze the hydrolysis of urea into carbon dioxide and ammonia, were isolated from mangrove sediments of Poovar (Trivandrum, India). Bacillus halodurans, strain PO15, isolated in this study with high urease (UA) activity (28 U/ml) was subjected to optimization using a Box-Behnken experimental design. Incubation variables included incubation period, pH, inoculation percentage and temperature. Significant factors identified based on the model were incubation period, pH, incubation temperature, and inoculum percentage; variations in these produced a tenfold increase in UA activity (295.80 U/ml). The specific activity of the purified UA enzyme was 62.34 U/mg and was found to be thermostable (active up to 60 °C). UA of B. halodurans PO15 has potential for microbial-induced biomineralization with a reduction of free Ca 2+ to about 82.8% ± 0.17%. The microbialinduced calcium precipitation (MICP) using the UA enzyme will potentially be beneficial in the process of biomineralization as well as for a variety of industrial applications.

show abstract

Section: Purification and Characterization Of Uamentioning

confidence: 91%

Section: Purification and Characterization Of Uamentioning

confidence: 94%

Section: Purification and Characterization Of Uamentioning

confidence: 99%

Section: Statistical Optimization For Urease Productionmentioning

confidence: 99%

See 2 more Smart Citations

Optimization of urease production by Bacillus halodurans PO15: a mangrove bacterium from Poovar mangroves, India

Nathan

Jasna

Parvathi

2020

Mar Life Sci Technol

View full text Add to dashboard Cite

show abstract

“…). Bacterial growth in most soils is often limited due to the lack of organic constituents.However, the constituents of cultivation media can affect the efficacy of MICPs[55,56]. De Muynck et al[48] revealed that nutritional composition have a profound impact on the morphological formation of CaCO 3 crystals.…”

mentioning

confidence: 99%

Bioprecipitation of calcium carbonate mediated by ureolysis: A review

Omoregie

Palombo

Nissom

2020

Environmental Engineering Research

View full text Add to dashboard Cite

Ureolysis-driven microbially induced carbonate precipitation (MICP) is a naturally occurring process facilitated through microbial activities and biogeochemical reactions to produce calcium carbonate (CaCO 3) mineral. MICP serves as an alternative ground improvement binder method to conventional technologies which is sustainable, requires low energy for its treatment process, results in a minimal carbon footprint and could offer economic benefits. In the last two decades, MICP has drawn great interest from the scientific community because of its practicality to stabilize granular soils, repair concrete cracks and remediate heavy metals. To obtain successful MICP application, it is vital to understand the conditions that favor its process. This paper, therefore, provides an overview of literature on CaCO 3 precipitation mediated by ureolysis-driven MICP and its mechanism. The review includes a discussion on sources of urease enzyme from microorganisms used to induce CaCO 3 crystal formation required for implementation of MCIP for ground improvement. Moreover, the key factors that influence the outcome of MICP and bio-engineering testing methods typically used to evaluate MICP performance are also highlighted. Finally, this review also provides insight on the current drawbacks (i.e. ammonium production, scaleup bioprocess and treatment cost) affecting MICP technology and recommendations for future consideration.

show abstract

Significance of Harvesting Green Energy: Emerging Trends and Prospects in Paddy Straw-Based Biohydrogen Technologies

Anwar,

Zafar,

Anwar

et al. 2024

Clean Energy Production Technologies

View full text Add to dashboard Cite

Optimization of process variables for enhanced production of urease by indigenous Aspergillus niger strains through response surface methodology

Cited by 18 publications

References 34 publications

Optimization of urease production by Bacillus halodurans PO15: a mangrove bacterium from Poovar mangroves, India

Optimization of urease production by Bacillus halodurans PO15: a mangrove bacterium from Poovar mangroves, India

Bioprecipitation of calcium carbonate mediated by ureolysis: A review

Significance of Harvesting Green Energy: Emerging Trends and Prospects in Paddy Straw-Based Biohydrogen Technologies

Contact Info

Product

Resources

About