Optimization of production conditions for β-mannanase using apple pomace as raw material in solid-state fermentation

Yin, Jun-Shuai; Liang, Qiwei; Li, Dongmei; Sun, Zilong

doi:10.1007/s13213-012-0449-0

Cited by 32 publications

(12 citation statements)

References 18 publications

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“…Recently, apple pomace and PKC were explored for production of fungal β‐mannanase in SSF by Yin et al. and Soni et al. , respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Several strains of Aspergillus, Trichoderma, and Sclerotium have been reported to be efficient mannanase producers utilizing a variety of mannan-rich crude substrates as carbon source, for instance, coffee waste [26], wheat bran [13], defatted copra [27] etc. Recently, apple pomace and PKC were explored for production of fungal β-mannanase in SSF by Yin et al [28] and Soni et al [15], respectively. Apple pomace has also been used as substrate for xylanase production using Aspergillus niger [29].…”

Section: Discussionmentioning

confidence: 99%

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Screening, statistical optimized production, and application of β‐mannanase from some newly isolated fungi

Soni

Rawat

Ahirwar

et al. 2016

Engineering in Life Sciences

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Eighty‐eight fungi isolated from soil and decaying organic matter were screened for mannanolytic activity. Twenty‐eight fungi produced extracellular mannanase on locust bean gum as evidenced by zone of hydrolysis produced on mannan agar gel. Six prominent producers, including four Fusarium species namely Fusarium fusarioides NFCCI 3282, Fusarium solani NFCCI 3283, Fusarium equiseti NFCCI 3284, Fusarium moniliforme NFCCI 3287 with Cladosporium cladosporioides NFCCI 3285 and Acrophialophora levis NFCCI 3286 produced the β‐mannanase in the range of 84–140 nkat/mL. All these grew well on particulate substrates in solid‐state fermentation (SSF), producing relatively higher titers on mannan‐rich palm kernel cake (PKC) and copra meal. Two high yielding strains, F. equiseti (1747 nkat/gds) and A. levis (897 nkat/gds) were selected for statistical optimization of mannanase on PKC. Interaction of two critical solid state fermentation parameters, pH and moisture on mannanase production by these two molds was studied by response surface method. Optimized production on PKC resulted in three‐ to fourfold enhancement in enzyme yield was observed in case of F. equiseti (5945 nkat/gds) and A. levis (4726 nkat/gds). HPLC analysis of mannan hydrolysate indicated that F. equiseti and A. levis mannanase performed efficient hydrolysis of konjac gum (up to 99%) with exclusive mannooligosaccahride (DP of 4) production. A seminative SDS‐PAGE revealed that A. levis and F. solani produced three isoforms, F. moniliforme produced two isoforms while F. fusarioides, F. equiseti, and C. cladosporioides produced a single enzyme.

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“…Recently, apple pomace and PKC were explored for production of fungal β‐mannanase in SSF by Yin et al. and Soni et al. , respectively.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Screening, statistical optimized production, and application of β‐mannanase from some newly isolated fungi

Soni

Rawat

Ahirwar

et al. 2016

Engineering in Life Sciences

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“…Apple pomace (AP) is the solid residue during apple juice extraction and a large number of this by-product was produced in the industry. Although AP contains some useful compositions such as water-soluble dietary fiber (SDF), it is usually abandoned as a waste, which may cause severe environmental problem (Yin et al 2013). AP was used to prepare some value-added products such as citric acid, feed and fertilizer in order to make full use of this resource and avoid possible pollution.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Functional Properties of Water‐Soluble Dietary Fiber from Apple Pomace

et al. 2014

J Food Process Engineering

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The extraction of water‐soluble dietary fiber (SDF) from apple pomace (AP) by cellulase, microwave‐ and ultrasound‐assisted methods was studied in comparison with conventional acid method. The functional properties of extracted SDFs were further evaluated, and the speciality of each method was clarified in order to suggest the right method to adopt for practice. Results indicated that AP is rich in SDF, which is significantly potential to be applied in food processing. Acid hydrolysis of AP gave 10.3% of SDF, whereas ultrasound‐assisted extraction (UAE) provided a yield of 16.4% after 40 min of sonication. Microwave‐assisted extraction (MAE) gave a slightly lower yield (14.9%) than UAE, but it showed a drastic efficiency, wherein the yield was achieved after 2 min of irradiation. The hydrolysis of AP by cellulase occurred with the highest SDF yield (18.7%) among all the methods used. The functional properties of SDF were improved by UAE, MAE and cellulase extraction, wherein the water retention and swelling capacities were increased more considerably than the oil retention capacity. Especially, cellulase showed a remarkable performance in improving the hydration property. Practical Applications A large amount of apple pomace (AP) was produced as the by‐product of apple processing in China. Its disposal is of significance in consideration of resource utilization and environmental protection. Therefore, the results of this study can promote the full use of AP and similar industrial wastes, and also help to avoid possible pollution. In addition, the comparison of acid, cellulase, microwave‐ and ultrasound‐assisted methods suggests the right method to adopt SDF extraction method for practice.

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“…N16-5 using the modifi ed alkaline Horikoshi-II medium (LIN et al, 2007), 3.78 U ml -1 by recombinant Escherichia coli in Luria-Bertani medium including 50 μg ml -1 kanamycin (KATROLIA et al, 2013), 1122 U mg -1 by recombinant Pichia pastoris in yeastpeptone-dextrose medium (LUO et al, 2012), 1231.41 U ml -1 by Bacillus subtilis Bs5 from konjac powder , and 561.3 U g -1 by Aspergillus niger SN-09 from apple pomace (YIN et al, 2012b).…”

mentioning

confidence: 99%

“…Purifi ed sugars in medium increase yield, but the cost also increases. Therefore, researchers have evaluated alternative complex carbon sources, such as molasses (OZTURK et al, 2010) and apple pomace, in order to decrease the fermentation costs (YIN et al, 2012b).…”

mentioning

confidence: 99%

Enhanced β-mannanase production from alternative sources by recombinant Aspergillus sojae

Yatmaz¹,

Karahalil²,

Germeç³

et al. 2016

Acta Alimentaria

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β-mannanases can degrade galactomannans to mannose and it has been used in various application areas. The aim of this study was to produce the β-mannanase from carob pod extract including different nitrogen sources. The best operation values for fermentation were determined to be 8% initial sugar concentration with 0.5% yeast extract, 100 r.p.m., and 7% inoculation rate, which yielded the maximum β-mannanase activity as 423.60 U ml -1 . Effects of nitrogen sources on β-mannanase activity were also studied and it reached 695.6 U ml -1 by using 0.5% of ammonium nitrate as the nitrogen source at the determined optimum conditions. Results also showed that meat bone meal and soybean meal could be used as cost effective nitrogen sources based on achieved β-mannanase activity.Keywords: β-mannanase, recombinant Aspergillus sojae, carbon sources, nitrogen sourcesMicrobial mannanase has gained signifi cant attention, since it degrades the complex polysaccharides of plant tissues into simple small molecules such as manno-oligosaccharides and mannose. For example, the increased availability and high specifi city of β-mannanases have been used for numerous applications, such as production of manno-oligosaccharides, pharmaceutical applications, instant coffee production, animal feeds, paper and pulp production, detergent formulations etc. ( VAN ZYL et al., 2010;LU et al., 2014). β-Mannanase could be generally produced by various Aspergillus species ( VAN ZYL et al., 2010) and the activity of the produced enzymes can differ from species to species. Also, other microorganisms have been evaluated for β-mannanase productions. CHEN and coworkers (2007) expressed Aspergillus sulphures β-mannanase gene in Pichia pastoris and the specifi c activity was found to be 366 U ml -1 in locust bean gum. OZTURK and co-workers (2010) used recombinant Aspergillus sojae ATCC11906 (AsT1) for β-mannanase production from molasses and found the highest activity value as 363 U ml -1. WANG and co-workers (2010) have found that Pantoea agglomerans A021 could produce β-mannanase from konjac powder and the maximum activity of purifi ed Man26P was 514 U mg -1. WU and co-workers (2011) used Aspergillus niger E-30 to produce the β-mannanase from peeled potato and the maximum β-mannanase activity value was 1067.5 U mg -1 by purifi ed enzyme solution

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Optimization of production conditions for β-mannanase using apple pomace as raw material in solid-state fermentation

Cited by 32 publications

References 18 publications

Screening, statistical optimized production, and application of β‐mannanase from some newly isolated fungi

Screening, statistical optimized production, and application of β‐mannanase from some newly isolated fungi

Extraction and Functional Properties of Water‐Soluble Dietary Fiber from Apple Pomace

Enhanced β-mannanase production from alternative sources by recombinant Aspergillus sojae

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