Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528

Lario, Luciana D.; Pillaca-Pullo, Omar; Sette, Lara Durães; Converti, Attílio; Spampinato, Claudia P.; Pessoa, Adalberto

doi:10.1016/j.btre.2020.e00546

Cited by 22 publications

(18 citation statements)

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“…This yeast can utilize a wide range of substrates, mono-and polysaccharides and waste materials while producing highvalue substances [6]. Besides carotenoids, these unicellular microorganisms are capable of producing a variety of biotechnologically important metabolites, such as lipids [7], ergosterol [8], enzymes [9], extracellular molecules [10] and polysaccharides [11].…”

Section: Introductionmentioning

confidence: 99%

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

et al. 2021

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Multifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be used in the food and feed industry and the pharmaceutical industry, as well as a source of biofuels. In the study, we examined the effect of different nitrogen sources, carbon sources and CN ratios on the co-production of intracellular lipids, carotenoids, β–glucans and extracellular glycolipids. Yeast strain R. kratochvilovae CCY 20-2-26 was identified as the best co-producer of lipids (66.7 ± 1.5% of DCW), exoglycolipids (2.42 ± 0.08 g/L), β-glucan (11.33 ± 1.34% of DCW) and carotenoids (1.35 ± 0.11 mg/g), with a biomass content of 15.2 ± 0.8 g/L, by using the synthetic medium with potassium nitrate and mannose as a carbon source. It was shown that an increased C/N ratio positively affected the biomass yield and production of lipids and β-glucans.

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Section: Introductionmentioning

confidence: 99%

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

et al. 2021

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“…High concentrations of easy assimilation saccharides have a negative effect on protease production despite stimulating growth [ 31 , 34 ]. The high amount of reducing sugars resulted from the hydrolysis of starch in wheat flour and corn powder may have led to carbon catabolite repression, suggesting that in the absence of glucose, protease plays a role in supplying peptides or amino acids as the carbon or energy source in addition to being a nitrogen source.…”

Section: Discussionmentioning

confidence: 99%

Optimization of protease production from Rhizomucor miehei Rm4 isolate under solid-state fermentation

Aljammas

Yazji

Azizieh

2022

Journal of Genetic Engineering and Biotechnology

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Background Protease is one of the most important industrial enzymes. The importance of protease bioproduction comes from meeting the increasing demand for this enzyme especially in the cheese industry. Rhizomucor miehei protease is the preferred substitute for the traditional rennet. Solid-state fermentation (SSF) shows promising results in enzyme production. An optimization strategy was applied to optimize the production of Rhizomucor miehei protease in a solid medium. The components of the fermentation medium were screened by using the one-factor-at-a-time (OFAT) approach. The optimization process then was performed by using the response surface methodology (RSM) approach based on five factors (fermentation time, temperature, pH, moisture content, nitrogen concentration) at five levels. Specific milk clotting activity and milk clotting activity/proteolytic activity ratio were considered as response variables in the optimization process. Results Among several combinations, wheat bran was selected as the best substrate. Casein was selected based on preliminary screening of nitrogen sources. The optimal conditions identified by RSM analysis were found to be 81.21 h, 41.11°C, 6.31, 80%, and 1.33% for fermentation time, temperature, pH, moisture content, and casein concentration, respectively. The performed fermentation process under the optimized conditions gave an enzymatic extract with the values of 5.11 mg/mL, 2258.13 Soxhlet unit/mL, 441.90 Soxhlet unit/mg, 1.14 protease unit/mg, and 388.66 for protein content, milk clotting activity, specific clotting activity, specific proteolytic activity, and milk clotting activity/proteolytic activity ratio, respectively. The aforementioned values were close to the predicted values. Conclusion The high milk clotting activity and the relatively low proteolytic activity signify higher specificity of the produced enzyme, which is favorable in cheese making. The observed results reveal the efficiency of the applied statistical approaches in obtaining desired values of response variables and minimizing experimental runs, as well as achieving good predictions for response variables.

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“…Different strains of R . mucilaginosa exhibit various capabilities, involving protease ( Lario et al., 2015 , 2020 ; Chaud et al., 2016 ), laccase ( Zhang et al., 2020 ) and cutinase ( Zhang et al., 2015 ). Table 3 summarizes different R .…”

Section: Beneficial Effect Of R Mucilaginosa On Pl...mentioning

confidence: 99%

“…(2020) LSL Enzyme producing Aldo-keto reductase Anello et al. (2020) CBMAI 1528 Enzyme producing Protease Lario et al. (2020) JM401 Enzyme producing Laccase Duarte et al., 2018 L7 Enzyme producing Protease Chaud et al.…”

Section: Beneficial Effect Of R Mucilaginosa On Pl...mentioning

confidence: 99%

Rhodotorula mucilaginosa—alternative sources of natural carotenoids, lipids, and enzymes for industrial use

Cheng

et al. 2022

Heliyon

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Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528

Cited by 22 publications

References 45 publications

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

Rhodotorula kratochvilovae CCY 20-2-26—The Source of Multifunctional Metabolites

Optimization of protease production from Rhizomucor miehei Rm4 isolate under solid-state fermentation

Rhodotorula mucilaginosa—alternative sources of natural carotenoids, lipids, and enzymes for industrial use

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