Optimization of Protease Production from Aspergillus Oryzae Sp. Using Box‐Behnken Experimental Design

Abstract

Protease production byAspergillus oryzaewas optimized in shake-flask cultures using Box-Behnken experimental design. An empirical model was developed through response surface methodology to describe the relationship between tested variable (peptone, glucose, soyabeanmeal and pH). Maximum enzyme activity was attained with Peptone at 4 g∕L; temperature at 30 °C glucose at 6 g∕L; 30 °C and pH at 10. Experimental verification of the model showed a validation of 95%, which is more than 3-fold increase compare to the basal medium.

RESPONSE SURFACE METHODOLOGY APPLIED TO THE EXTRACTION OF PHENOLIC COMPOUNDS FROM JATROPHA CURCAS LINN. LEAVES USING SUPERCRITICAL CO2 WITH A METHANOL CO‐SOLVENT

“…The RSM literature, however, recommends using the analytical approach, i.e., taking the partial derivative ∂ Y i /∂ X ij ; where i represents the solute GA, CG or EA, and j represents the particular variable(s) considered in , setting it equal to zero and solving for X j(j = 1,2,3) (Annadurai 2000; Ge et al. 2002; Myers and Montgomery 2002; Babu et al. 2007; Lin et al.…”

Optimization study of BTEX extraction from soils spiked with two kinds of Brazilian diesel by HS SPME using Box–Behnken experimental design and multi-response analysis

“…This design allows the construction of a second-order polynomial model which can be used to describe or optimize the extraction process. 28,29 The polynomial equation has the form of eqn (3):…”

The use of serine protease from Yarrowia lipolytica yeast in the production of biopeptides from denatured egg white proteins

“…The use of proteases in industrial processes depends on their substrate specificity, preference for the position of the hydrolyzed peptide bond, the types of substances inhibiting the activity of the enzyme, as well as stability across a wide range of temperature and pH. One of the most important factors is their cost, dependent on the availability of sources and the procedure of isolation (Srinu Babu et al, 2007).…”