Optimization of reagent concentration for radioiodination of rat C-peptide II in development of radioimmunoassay procedure for rats

Manupriya, BR; Paradkar, Shalaka; Lathika,; Patil, Shrikant L; Somashekarappa, H.M.; Shenoy, Bhasker K

doi:10.4103/rpe.rpe_11_18

Cited by 2 publications

(1 citation statement)

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“…The SFMs were labeled with 131 I by a modified chloramine-T method, 33 as illustrated in Figure 1C,D. Briefly, 8.0 mg of SFMs was dispersed in 160.0 μL of PBS (pH = 7.4, 0.0067 M).…”

Section: ■ Introductionmentioning

confidence: 99%

¹³¹I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma

Shen

et al. 2022

ACS Appl. Mater. Interfaces

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Transarterial radioembolization (TARE) is a promising technology in hepatocellular carcinoma (HCC) therapy, which utilizes radionuclide-labeled microspheres to achieve arterial embolization and internal irradiation. However, the therapeutic effect of liver cancer can be affected by low radionuclide labeling rate and stability, as well as poor biocompatibility, and nonbiodegradability of microspheres. Here, 131 I-labeled silk fibroin microspheres ( 131 I-SFMs) were developed as radioembolization material for effective TARE therapy against HCC. Silk fibroin rich in 10.03% of tyrosine was extracted from silkworm cocoons and then emulsified and genipin-crosslinked to prepare SFMs. SFMs show a good settlement rate, biodegradability, hemocompatibility, and low cytotoxicity. Afterward, 131 I-SFMs were obtained by radiolabeling 131 I onto the SFMs through the chloramine-T method. 131 I-SFMs possess a high 131 I labeling rate of over 84% and good radioactive stability and are thus conducive to internal radiotherapy. Significantly, 131 I-SFMs with diameters around 11 μm were successfully radioembolized at the hepatic artery. 131 I-SFMs were diffused in the liver, indicating the favorable biodistribution and biosafety in vivo. Based on the combination of embolization and local radiotherapy, the administration of 131 I-SFMs shows a favorable inhibitive effect against the progression of HCC. Overall, the newly developed 131 I-SFMs as radioembolization microspheres provide a promising application for effective TARE therapy against liver cancer.

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“…The SFMs were labeled with 131 I by a modified chloramine-T method, 33 as illustrated in Figure 1C,D. Briefly, 8.0 mg of SFMs was dispersed in 160.0 μL of PBS (pH = 7.4, 0.0067 M).…”

Section: ■ Introductionmentioning

confidence: 99%

¹³¹I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma

Shen

et al. 2022

ACS Appl. Mater. Interfaces

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Development of a magnetizable cellulose particle-based immunoradiometric assay for quantification of C-peptide in rat serum

Manupriya

Paradkar²,

Ghodke³

et al. 2023

J Radioanal Nucl Chem

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Optimization of reagent concentration for radioiodination of rat C-peptide II in development of radioimmunoassay procedure for rats

Cited by 2 publications

References 0 publications

¹³¹I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma

¹³¹I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma

Development of a magnetizable cellulose particle-based immunoradiometric assay for quantification of C-peptide in rat serum

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Optimization of reagent concentration for radioiodination of rat C-peptide II in development of radioimmunoassay procedure for rats

Cited by 2 publications

References 0 publications

131I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma

131I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma

Development of a magnetizable cellulose particle-based immunoradiometric assay for quantification of C-peptide in rat serum

Contact Info

Product

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¹³¹I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma

¹³¹I-Labeled Silk Fibroin Microspheres for Radioembolic Therapy of Rat Hepatocellular Carcinoma