Optimization of Reducing Sugar Production from Acid Hydrolysis of Sugarcane Bagasse by Box Behnken Design

Abstract

Sugarcane bagasse is a low-cost and abundant of bio-resources in sugar mill industry. The cellulose of bagasse can be converted into reducing sugar that has been applied in food and biological process. Statistically based Box-Behken Design (BBD) was selected for the optimization of reducing sugar production from acid hydrolysis of sugarcane bagasse. Concentration of H 2 SO 4 , hydrolysis time, and the ratio of raw material to solvent were identified for significant effects on reducing sugar production. Response surface method by BBD was employed to develop a mathematical model for predicting the optimal condition of acid hydrolysis process. The value of R 2 (0.981) for the presented mathematical model indicates the high correlation between experimental and predicted values. The optimization of concentration of H 2 SO 4 , hydrolysis time, and ratio of raw material to solvent are 27.697 %w/v, 160 min, and 1:29.827 g/ml respectively, which under this condition, it could produced 14.808% of reducing sugar from sugarcane bagasse.  Index Terms-sugarcane bagasse, reducing sugar, acid hydrolysis, Box-Behnken design.

Optimization of acid pretreatment and enzymatic hydrolysis on the production of ethanol fuel from waste banana peels

“…After acid pretreatment and enzymatic hydrolysis, the determination of reducing sugar concentration in the feedstock before fermentation was by DNS method. 18,19 The recovery of ethanol after fermentation was by double distillation and the determination of ethanol was by gas chromatography. 20…”