Optimization of l‐arginine purification from Corynebacterium crenatum fermentation broth

Abstract

Abstractl‐Arginine has many special physiological and biochemical functions, with wide applications in the food and pharmaceutical industry. Few studies on the purification of l‐arginine from fermentation broth have been conducted; however, none of them were systematic enough for industrial scale‐up. Therefore, it is necessary to develop a highly efficient and systematic process for the purification of l‐arginine from fermentation broth. In this study, we screened out a cation exchange resin, D155, having high exchange capacity, high selectivity, and easy elution capacity, and analyzed its adsorption isotherm, thermodynamics, and kinetics using different models. Further, the process parameters of fixed‐bed ion exchange adsorption and elution were optimized, and the penetration curve during the operation was modeled. Based on the fixed‐bed ion‐exchange parameters, a 30‐column continuous ion‐exchange system was designed, and the flow velocity in each zone was optimized. Finally, to obtain a high purity of l‐arginine, the purification tests were conducted using anion exchange resin 711, and an l‐arginine yield of 99.1% and purity of 98.5% was obtained. This effective and economical method also provides a promising strategy for separation of other amino acids from the fermentation broth, which is of great significance to the l‐arginine fermentation industry.

An Integrated Chromatographic Strategy for the Large-Scale Extraction of Ergosterol from Tulasnellaceae sp.

“…For the separation and purification of steroids (such as ergosterol) from plants, conventional methods such as thin-layer chromatography and silica gel column chromatography are commonly utilized [9][10][11] as these procedures only require basic equipment and are straightforward to implement. The separation of high-purity chemicals, however, continues to be problematic due to low separation repeatability and the lack of online detection methods.…”

“…The separation of high-purity chemicals, however, continues to be problematic due to low separation repeatability and the lack of online detection methods. High-speed countercurrent chromatography (HSCCC) has recently gained popularity for the separation of ergosterol from microbial samples and natural products [10,11], but HSCCC requires complex partition coefficient testing procedures and low separation resolution, making the separation and purification process difficult to simplify [12][13][14].…”

Evaluation of multiple hydrophilic interaction chromatography columns and surrogate matrix for arginine quantification in saliva by high‐resolution mass spectrometry

“…Therefore, a method to determine arginine concentrations in saliva as a biomarker could be very important for various biological studies including oral health monitoring. So far, most arginine analysis methodologies have focused on the quantitation of drugs, biological samples such as urine or plasma [9,10], animal feed [11], grape juice and wine [12,13], plants and food [14,15] and fermentation broth [16].…”