2001
DOI: 10.1081/ddc-100105184
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Optimization of Selected Chromatographic Responses Using a Designed Experiment at the Fine-Tuning Stage in Reversed-Phase High-Performance Liquid Chromatographic Method Development

Abstract: This study evaluated the applicability of a designed experiment at the fine-tuning stage in reversed-phase high-performance liquid chromatographic (HPLC) method development. Using acetaminophen, theophylline, and caffeine as model drugs, a 3(2) factorial design was used to optimize selected chromatographic responses. The effects of the ratio of water to acetonitrile (%v/v) in the mobile phase and mobile phase flow rate on the theoretical plate number of acetaminophen peak, capacity factor of acetaminophen, res… Show more

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Cited by 5 publications
(1 citation statement)
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“…First, a 10-experiment set Plackett-Burman design was used to screen the four operating factors. This type of design is called the fractional factorial design [9], and has been used elsewhere in method development and validation [10][11][12][13][14][15][16]. Plackett-Burman designs are often used to screen a number of factors using a relatively small number of experiments to identify the factors that have the greatest effect on the response variables.…”
Section: Introductionmentioning
confidence: 99%
“…First, a 10-experiment set Plackett-Burman design was used to screen the four operating factors. This type of design is called the fractional factorial design [9], and has been used elsewhere in method development and validation [10][11][12][13][14][15][16]. Plackett-Burman designs are often used to screen a number of factors using a relatively small number of experiments to identify the factors that have the greatest effect on the response variables.…”
Section: Introductionmentioning
confidence: 99%