Optimization of serum proteome profiling of healthy humans

Pakharukova, Natalia; Пастушкова, Л. Х.; Trifonova, Oxana P.; Pyatnitsky, Mikhail; Vlasova, Maria; Nikitin, I. P.; Moshkovsky, S. A.; Nikolayev, E. N.; Ларина, И. М.

doi:10.1134/s0362119709030116

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…The CVs for the normalized protein/peptide peak intensities were 7% [19]. However, later studies showed that the CVs were between 20% and 30% for both manual and robotic assays [20,21]. There are about 40 reports on using the ClinProt system in discovery of potential biomarkers of human diseases, such as oral cancer [19], head and neck cancer [20], and nephrotic syndrome [22].…”

Section: Quantitative Issues In the Maldi-tof Mass Spectramentioning

confidence: 99%

Advances in MALDI Mass Spectrometry in Clinical Diagnostic Applications

Wong

Poon

2013

Chemical Diagnostics

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The concept of matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) was first reported in 1985. Since then, MALDI MS technologies have been evolving, and successfully used in genome, proteome, metabolome, and clinical diagnostic research. These technologies are high-throughput and sensitive. Emerging evidence has shown that they are not only useful in qualitative and quantitative analyses of proteins, but also of other types of biomolecules, such as DNA, glycans, and metabolites. Recently, parallel fragmentation monitoring (PFM), which is a method comparable to selected reaction monitoring, has been reported. This highlights the potentials of MALDI-TOF/TOF tandem MS in quantification of metabolites. Here we critically review the applications of the major MALDI MS technologies, including MALDI-TOF MS, MALDI-TOF/TOF MS, SALDI-TOF MS, MALDI-QqQ MS, and SELDI-TOF MS, to the discovery and quantification of disease biomarkers in biological specimens, especially those in plasma/serum specimens. Using SELDI-TOF MS as an example, the presence of systemic bias in biomarker discovery studies employing MALDI-TOF MS and its possible solutions are also discussed in this chapter. The concepts of MALDI, SALDI, SELDI, and PFM are complementary to each other. Theoretically, all these technologies can be combined, leading to the next generation of the MALDI MS technologies. Real applications of MALDI MS technologies in clinical diagnostics should be forthcoming.

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Section: Quantitative Issues In the Maldi-tof Mass Spectramentioning

confidence: 99%

Advances in MALDI Mass Spectrometry in Clinical Diagnostic Applications

Wong

Poon

2013

Chemical Diagnostics

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show abstract

“…, low precision of measurement), such that the data acquired by the methodology was treated qualitatively. The technological advances of the MALDI technology, such as the use of high repetition rate solid-state laser, and improved instrument design, have increased the precision of measurement to less than 20% (demonstrated in a study using CLINPROT approach) [ 79 ]; this permits semi-quantitative measurement on biological systems by MALDI-MS.…”

Section: Top-down Proteomic Profiling By Laser Mass Spectrometrymentioning

confidence: 99%

Mass Spectrometry-Based Proteomics for Pre-Eclampsia and Preterm Birth

Law

Han

Tong

et al. 2015

IJMS

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Pregnancy-related complications such as pre-eclampsia and preterm birth now represent a notable burden of adverse health. Pre-eclampsia is a hypertensive disorder unique to pregnancy. It is an important cause of maternal death worldwide and a leading cause of fetal growth restriction and iatrogenic prematurity. Fifteen million infants are born preterm each year globally, but more than one million of those do not survive their first month of life. Currently there are no predictive tests available for diagnosis of these pregnancy-related complications and the biological mechanisms of the diseases have not been fully elucidated. Mass spectrometry-based proteomics have all the necessary attributes to provide the needed breakthrough in understanding the pathophysiology of complex human diseases thorough the discovery of biomarkers. The mass spectrometry methodologies employed in the studies for pregnancy-related complications are evaluated in this article. Top-down proteomic and peptidomic profiling by laser mass spectrometry, liquid chromatography or capillary electrophoresis coupled to mass spectrometry, and bottom-up quantitative proteomics and targeted proteomics by liquid chromatography mass spectrometry have been applied to elucidate protein biomarkers and biological mechanism of pregnancy-related complications. The proteomes of serum, urine, amniotic fluid, cervical-vaginal fluid, placental tissue, and cytotrophoblastic cells have all been investigated. Numerous biomarkers or biomarker candidates that could distinguish complicated pregnancies from healthy controls have been proposed. Nevertheless, questions as to the clinically utility and the capacity to elucidate the pathogenesis of the pre-eclampsia and preterm birth remain to be answered.

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“…The main attention has focused on technologies for the direct profiling of serum samples using magnetic beads with different functional surfaces and microchromatographic C18 zip-tips. 197 Another investigation performed in IBChPh RAS, has shown that mass spectrometry offers new possibilities for the proteomic analysis of exhaled breath condensate (EBC) as a simple non-invasive method of diagnosing respiratory diseases. 198 This method can be an alternative to the traditional endoscopic method and includes trypsinolysis of concentrated protein mixture, analysis of peptides by nanoflow HPLC-MS/MS with a 7-Tesla Finnigan LTQ-FT mass spectrometer and suitable bioinformatics.…”

Section: Medical Chemistrymentioning

confidence: 99%

Mass Spectrometry in Russia

Заикин

Сысоев

2013

Eur J Mass Spectrom (Chichester)

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The present review covers the main research in the area of mass spectrometry from the 1990s which was about the same time as the Russian Federation emerged from the collapse of the Soviet Union (USSR). It consists of two main parts-application of mass spectrometry to chemistry and related fields and creation and development of mass spectrometric technique. Both traditional and comparatively new mass spectrometric methods were used to solve various problems in organic chemistry (reactivity of gas-phase ions, structure elucidation and problems of identification, quantitative and trace analysis, differentiation of stereoisomers, derivatization approaches etc.), biochemistry (proteomics and peptidomics, lipidomics), medical chemistry (mainly the search of biomarkers, pharmacology, doping control), environmental, petrochemistry, polymer chemistry, inorganic and physical chemistry, determination of natural isotope ratio etc. Although a lot of talented mass spectrometrists left Russia and moved abroad after the collapse of the Soviet Union, the vitality of the mass spectral community proved to be rather high, which allowed the continuation of new developments in the field of mass spectrometric instrumentation. They are devoted to improvements in traditional magnetic sector mass spectrometers and the development of new ion source types, to analysis and modification of quadrupole, time-of-flight (ToF) and ion cyclotron resonance (ICR) analyzers. The most important achievements are due to the creation of multi-reflecting ToF mass analyzers. Special attention was paid to the construction of compact mass spectrometers, particularly for space exploration, of combined instruments, such as ion mobility spectrometer/mass spectrometer and accelerating mass spectrometers. The comparatively young Russian Mass Spectrometry Society is working hard to consolidate the mass spectrometrists from Russia and foreign countries, to train young professionals on new appliances and regularly holds conferences on mass spectrometry. For ten years, a special journal Mass-spektrometria has published papers on all disciplines of mass spectrometry.

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Optimization of serum proteome profiling of healthy humans

Cited by 4 publications

References 11 publications

Advances in MALDI Mass Spectrometry in Clinical Diagnostic Applications

Advances in MALDI Mass Spectrometry in Clinical Diagnostic Applications

Mass Spectrometry-Based Proteomics for Pre-Eclampsia and Preterm Birth

Mass Spectrometry in Russia

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