Optimization of Silkworm Sericin Extraction Attacus atlas and Samia cynthia ricini Using Response Surface Methodology

Endrawati, Yuni Cahya; Solihin, Dedy Duryadi; Suryani, Ani; Darmawan, Noviyan Darmawan; Suparto, Irma Herawati; Rahmantika, Bella Fitri

doi:10.22146/agritech.71950

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Despite limited evidence, the potential use of the non-mulberry cocoon type is exemplified by Attacus atlas , Cricula trifenestrata , and Samia ricini in terms of the quality of the silk fiber and its protein components, such as sericin and fibroin. 7 , 9 , 10 …”

Section: Introductionmentioning

confidence: 99%

Effect of natural silk fibers and synthetic fiber-reinforced composites on the cytotoxicity of fibroblast cell lines

Annisa,

Irnawati,

Siswomihardjo

et al. 2024

J Dent Res Dent Clin Dent Prospects

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Background. Synthetic fibers have many benefits in clinical practice; however, they cause microplastic pollution, and their unaffordable price increases treatment costs. Natural silk fibers require biocompatibility assessment. This study investigated the effects of natural and synthetic fiber-reinforced composites (FRCs) on the cytotoxicity of fibroblast cell lines. Methods. Three commercial synthetic fibers (polyethylene, quartz, and E-glass) and two silk fibers from Bombyx mori and Samia ricini cocoons were employed. These fibers were made into FRC samples (n=6) by impregnation in flowable composite using a brass mold (25×2×2 mm). NIH/3T3 mouse fibroblasts were cultured in Dulbecco’s modified eagle medium, supplemented, and seeded in 2×104 cells/mL. They were stored at 37 °C under 5% CO2 for 24 hours. The FRC samples were made into powder, eluted in dimethylsulfoxide, continued with PBS, supplemented with Dulbecco’s modified eagle medium (DMEM), and exposed to cells for 24 hours. Blank (medium only) and control (cells and medium) samples were included. Subsequently, MTT was added for 4 h and read by enzyme-linked immunosorbent assay (λ=570 nm). Cell viability (%) was calculated and analyzed using one-way ANOVA (α=0.05). Results. All groups of FRCs showed>80% cell viability. One-way ANOVA showed no significant difference between FRC groups regarding the viability of fibroblast cell lines (P>0.05). Conclusion. Both natural silk and synthetic fibers exhibit low cytotoxicity to fibroblast cell lines. B. mori and S. ricini silk fibers showed the potential to be used as alternative synthetic fibers.

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Section: Introductionmentioning

confidence: 99%

Effect of natural silk fibers and synthetic fiber-reinforced composites on the cytotoxicity of fibroblast cell lines

Annisa,

Irnawati,

Siswomihardjo

et al. 2024

J Dent Res Dent Clin Dent Prospects

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“…Structurally, silk fibers consist of fibroin protein fibers coated with sericin as an adhesive [12]. When applied as textiles, sericin needs to be removed as it can affect the quality and application of the fibers [13]. This process is known as degumming.…”

Section: Introductionmentioning

confidence: 99%

Physical Characterization of Indonesian Eri Silk Fiber Derived from Novel Strains of Samia cynthia ricini

Maldia,

Darmawan,

Endrawati

et al. 2023

Egypt. J. Chem.

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Eri silk fibers are produced by the silkworm Samia cynthia ricini, which has been extensively cultivated in several tropical countries due to its adaptability to elevated temperatures and humidity, as well as the ample availability of its food sources. Currently, we have developed six new strains of S. ricini with extraordinary cocoon productivity and the ability to survive in marginal/extreme areas (high temperature and low humidity), namely Joglo, Jopati, Pasopati, Prasojo, Progo, and Tawang strains, respectively. This research analyzes the physical characterization of these Eri silk fibers, including the thermal characteristics, crystallinity structure, morphology, and functional groups of the silk fibers. The surface morphology of the degummed Eri silk fibers reveals the presence of separated fiber filaments with a smooth surface, ranging in diameter from 14 -33 µm. The tensile strength and elongation of the silk fibers from the new strains do not show a significant difference (P > 0.05 %), around 350 -497 MPa and 15 -26 %, respectively. In addition, the Fourier transform infrared spectrometer was also utilized to investigate the structural differences of Eri silk fibers. The results show absorption bands at 1623 cm -1 , 1513 cm -1 , 1219 cm -1 , and 703 cm -1 indicating vibration of C=O (Amide I), N-H (Amide II), and C-N (Amide III) groups indicate the presence of β-sheet structures in the silk fibers. X-ray diffractograms of Eri silk fibers show peaks at 16.54° and 20.40° that correspond to the β-sheet crystalline structure. The new strain fibers have no significant structural disparities compared to the general S. ricini silk fibers. The thermal characteristics of the Eri silk fibers from the new strains are similar, with water removal occurring at temperatures around 100 to 125 °C and degradation temperatures around 369 -373 °C. However, the Pasopati strain exhibits the highest heat capacity increment (ΔCp), 1.266 J g -1 °C-1 . Based on the comparative results, Eri silk fiber from novel strains of S. ricini reported can be utilized more extensively in the textile industry and biomaterial applications.

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Optimization of Silkworm Sericin Extraction Attacus atlas and Samia cynthia ricini Using Response Surface Methodology

Cited by 2 publications

References 17 publications

Effect of natural silk fibers and synthetic fiber-reinforced composites on the cytotoxicity of fibroblast cell lines

Effect of natural silk fibers and synthetic fiber-reinforced composites on the cytotoxicity of fibroblast cell lines

Physical Characterization of Indonesian Eri Silk Fiber Derived from Novel Strains of Samia cynthia ricini

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