Optimization of single-tube nested PCR for the detection of Echinococcus spp.

Zhang, Xueyong; Jian, Yingna; Li, Zhi; Ding, Hong; Guo, Zhihong; Fu, Yong

doi:10.1016/j.exppara.2023.108494

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…DNA. STNPCR displayed 100 times increased sensitivity compared to conventional PCR, making it suitable for gene sequencing and epidemiological investigations [ 68 ]. Genetic diversity and evolution can be studied through the ability of Echinococcus spp.…”

Section: Methods For Diagnosis Of Cystic Echinococcosis In Intermedia...mentioning

confidence: 99%

Advances in Detecting Cystic Echinococcosis in Intermediate Hosts and New Diagnostic Tools: A Literature Review

Hajjafari,

Sadr,

Santucciu

et al. 2024

Veterinary Sciences

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Cystic echinococcosis (CE) is a zoonotic disease affecting humans and animals. Despite a lack of clarity about many details of parasite–intermediate host interactions, the nature of the immune responses triggered by hydatid infection has revealed new perspectives. This study discusses the latest advances in elucidating the immunologic mechanism of echinococcosis and its detection and potential approaches to enhance serodiagnosis accuracy. Moreover, nanobiosensors have been evaluated according to their potential to improve treatment efficiency and aid in an early diagnosis of cystic echinococcosis. The serum of an intermediate host can diagnose CE by analyzing antibodies induced by Echinococcus granulosus. Among the most notable features of this method are its noninvasive ability and high sensitivity, both of which make it an excellent tool for clinical diagnosis. Several serological tests, including ELISAs and immunoblotting, can detect these antibodies to assess the disease’s state and determine the treatment outcome. A thorough understanding of what cross-reactivity means and the stage of the disease are crucial to interpreting serological results. Nanobiosensors have also proven better than conventional biosensors in detecting hydatid cysts. Additionally, they are highly sensitive and versatile when detecting specific biomarkers, improving diagnostic accuracy. These immunomodulatory molecules, induced by E. granulosus, are a good candidate for diagnosing cystic echinococcosis because they alter intermediate host immune responses. Hydatid cyst detection is also enhanced through nanobiosensors, which provide better accuracy.

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Section: Methods For Diagnosis Of Cystic Echinococcosis In Intermedia...mentioning

confidence: 99%